摘要
为筛选火龙果(Hylocereus undatus)优良种质资源,对22份种质资源果实的表型性状、农艺性状、品质性状进行遗传多样性分析。结果表明,火龙果种质资源果实的表型性状、农艺性状和品质性状具有丰富的遗传多样性和较高的变异性,表型性状的多样性指数(H′)为0~1.04,品质性状为0.40~2.01;农艺性状的变异系数(CV)为0.06~0.38,品质性状为0.01~0.62。聚类分析表明,在遗传距离为15时,火龙果22份种质资源可分为5类,说明不同资源间亲缘关系较远。这为发掘火龙果的育种潜力,筛选优异基因资源,改良种质奠定了基础。
In order to screen excellent germplasm of Hylocereus undatus, the fruit genetic diversity analysis in phenotypic traits, agronomic traits and quality traits of 22 germplasms were studied. The results showed that the phenotypic, agronomic and quality traits of H. undatus fruit had abundant genetic diversity and high variability.The diversity indexes(H') of fruit phenotypic and quality traits were 0-1.04 and 0.40-2.01, respectively. The coefficient of variation(CV) of agronomic traits and quality traits were 0.01-0.62 and 0.06-0.38, respectively.Cluster analysis showed that 22 germplasms of H. undatus could be devided into 5 groups at genetic distance of15, indicating that different germplasms were far related. These would lay a foundation for exploring the breeding potential of H. undatus, screening excellent genetic resources and improving germplasm.
引文
[1]NERD A,SITRIT Y,KAUSHIK R A,et al.High summer temperatures inhibit flowering in vine pitaya crops(Hylocereus spp.)[J].Sci Hort,2002,96(1/2/3/4):343-350.doi:10.1016/S0304-4238(02)00093-6.
[2]CAI Y Q,XIANG Q Y,CHEN J L,et al.Analysis of nutritional components in pitaya fruit[J].Nonwood For Res,2008,26(4):53-56.doi:10.3969/j.issn.1003-8981.2008.04.012.蔡永强,向青云,陈家龙,等.火龙果的营养成分分析[J].经济林研究,2008,26(4):53-56.doi:10.3969/j.issn.1003-8981.2008.04.012.
[3]LI W Y,PENG Z J,WANG B,et al.Content and composition of soluble sugar and organic acid in flesh of different pitaya varieties[J].Guizhou Agric Sci,2010,38(11):215-217.doi:10.3969/j.issn.1001-3601.2010.11.067.李文云,彭志军,王彬,等.火龙果不同品种(品系)果肉糖、酸含量及组成分析[J].贵州农业科学,2010,38(11):215-217.doi:10.3969/j.issn.1001-3601.2010.11.067.
[4]XUE W D,WANG A G.Preliminary report on introduction and cultivation of Taiwan pitaya[J].S Chin Fruits,2003,32(2):34-35.doi:10.3969/j.issn.1007-1431.2003.02.025.薛卫东,王阿桂.台湾火龙果引种栽培初报[J].中国南方果树,2003,32(2):34-35.doi:10.3969/j.issn.1007-1431.2003.02.025.
[5]MIZRAHI Y,NERD A,NOBEL P S.Cacti as crops[J].Hort Rev,1997,18:291-391.
[6]YAN C R.Handbook of Agricultural Extension for New Fruit Tree Cultivation[M].Pingdong:Agricultural Extension Committee of National Pingtung University of Science and Technology,2002:36.颜昌瑞.新兴果树栽培农业推广手册[M].屏东:国立屏东科技大学农业推广委员会,2002:36.
[7]DENG R J,FAN J X,CAI Y Q.Present research status and industrial development of pitaya at home and abroad[J].Guizhou Agric Sci,2011,39(6):188-192.doi:10.3969/j.issn.1001-3601.2011.06.052.邓仁菊,范建新,蔡永强.国内外火龙果研究进展及产业发展现状[J].贵州农业科学,2011,39(6):188-192.doi:10.3969/j.issn.1001-3601.2011.06.052.
[8]WANG Z,WANG L J,CAI Y Q,et al.Research progress on nutrient components and functional substances of pitaya[J].S Chin Fruits,2014,43(5):25-29.doi:10.13938/j.issn.1007-1431.2014.05.018.王壮,王立娟,蔡永强,等.火龙果营养成分及功能性物质研究进展[J].中国南方果树,2014,43(5):25-29.doi:10.13938/j.issn.1007-1431.2014.05.018.
[9]TIAN X M,LI H L,HE Y,et al.Research progress of pitaya[J].NHort,2015(18):188-193.doi:10.11937/bfyy.201518051.田新民,李洪立,何云,等.火龙果研究现状[J].北方园艺,2015(18):188-193.doi:10.11937/bfyy.201518051.
[10]ZHENG W,WANG B.Biological characteristics,health value and development prospect of pitaya[J].SW Hort,2004,32(3):47-48.郑伟,王彬.火龙果生物学特性、保健价值及其发展前景[J].西南园艺,2004,32(3):47-48.
[11]HUANG F Z,LU G F,HUANG L F,et al.Collection and evaluation of pitaya germplasm resources[J].SW Chin J Agric Sci,2016,29(4):920-924.doi:10.16213/j.cnki.scjas.2016.04.035.黄凤珠,陆贵锋,黄黎芳,等.火龙果种质资源收集保存与初步评价[J].西南农业学报,2016,29(4):920-924.doi:10.16213/j.cnki.scjas.2016.04.035.
[12]WANG Q Q,HU W B,PU W H,et al.Analysis of SRAP genetic diversity of 32 pitaya germplasm[J/OL].Chin J Trop Crops,2018:1-7[2018-05-15].http://kns.cnki.net/kcms/detail/46.1019.S.20180515.1124.002.html.汪琴琴,胡文斌,濮文辉,等.32份火龙果种质资源的SRAP遗传多样性分析[J/OL].热带作物学报,2018:1-7[2018-05-15].http://kns.cnki.net/kcms/detail/46.1019.S.20180515.1124.002.html.
[13]JUNQUEIRA K P,FALEIRO F G,JUNQUEIRA N T V,et al.Genetic diversity of native pitaya native from Brazilian savannas with basis on RAPD markers[J].Rev Bras Frutic,2010,32(3):819-824.doi:10.1590/S0100-29452010005000104.
[14]ZHAN Y F,YANG H,TU X M,et al.Genetic diversity of hot pepper germplasm resources and its cluster analysis[J].Guizhou Agric Sci,2010,38(11):12-15.doi:10.3969/j.issn.1001-3601.2010.11.004.詹永发,杨红,涂祥敏,等.辣椒品种资源的遗传多样性和聚类分析[J].贵州农业科学,2010,38(11):12-15.doi:10.3969/j.issn.1001-3601.2010.11.004.
[15]FENG X J,WU Y Y,LIU Z W,et al.Comparative test of new varieties of north-transport type cowpea[J].Guangdong Agric Sci,2011,38(21):56,62.doi:10.3969/j.issn.1004-874X.2011.21.018.冯学杰,吴月燕,刘振文,等.北运型豇豆新品种比较试验[J].广东农业科学,2011,38(21):56,62.doi:10.3969/j.issn.1004-874X.2011.21.018.
[16]National Health and Family Planning Commission of PRC,China Food and Drug Administration.GB 5009.5-2016 National food safety standard:Determination of proteins in foods[S].Beijing:Standards Press of China,2017.中华人民共和国国家卫生和计划生育委员会,国家食品药品监督管理总局.GB 5009.5-2016食品安全国家标准食品中蛋白质的测定[S].北京:中国标准出版社,2017.
[17]National Health and Family Planning Commission of PRC.GB5009.88-2014 National food safety standards:Determination of dietary fiber in foods[S].Beijing:Standards Press of China,2016.中华人民共和国国家卫生和计划生育委员会.GB 5009.88-2014食品安全国家标准食品中膳食纤维的测定[S].北京:中国标准出版社,2016.
[18]National Health and Family Planning Commission of PRC.GB5009.3-2016 National food safety standard:Determination of moisture in foods[S].Beijing:Standards Press of China,2017.中华人民共和国国家卫生和计划生育委员会.GB 5009.3-2016食品安全国家标准食品中水分的测定[S].北京:中国标准出版社,2017.
[19]National Health and Family Planning Commission of PRC.GB5009.4-2016 National food safety standards for the determination of ash in foods[S].Beijing:Standards Press of China,2017.中华人民共和国国家卫生和计划生育委员会.GB 5009.4-2016食品安全国家标准食品中灰分的测定[S].北京:中国标准出版社,2017.
[20]General Administration of Quality Supervision,Inspection and Quarantine of the People’s Republic of China,Standardization Administration of the People’s Republic of China.GB/T 6433-2006Determination of crude fat in feeds[S].Beijing:Standards Press of China,2006.中华人民共和国国家质量监督检验检疫总局,中国国家标准化管理委员会.GB/T 6433-2006饲料中粗脂肪的测定[S].北京:中国标准出版社,2006.
[21]General Administration of Quality Supervision,Inspection and Quarantine of the People’s Republic of China,Standardization Administration of the People’s Republic of China.GB/T 12456-2008 Determination of total acid in foods[S].Beijing:Standards Press of China,2009.中华人民共和国国家质量监督检验检疫总局,中国国家标准化管理委员会.GB/T 12456-2008食品中总酸的测定[S].北京:中国标准出版社,2009.
[22]National Health Commission of the People’s Republic of China.GB14754-2010 National food safety standard:Food additive,Vitamin C(Ascorbic Acid)[S].Beijing:Standards Press of China,2011.中华人民共和国卫生部.GB 14754-2010食品安全国家标准食品添加剂维生素C(抗坏血酸)[S].北京:中国标准出版社,2011.
[23]The Ministry of Agriculture of the People’s Republic of China.NY/T2742-2015 Determination of soluble sugar in fruits and derived products-3,5-dinitrosalicylic acid colorimetry[S].Beijing:China Agriculture Press,2015.中华人民共和国农业部.NY/T 2742-2015水果及制品可溶性糖的测定3,5-二硝基水杨酸比色法[S].北京:中国农业出版社,2015.
[24]National Health Commission of the People’s Republic of China,Standardization Administration of the People’s Republic of China.GB/T 5009.38-2003 Method for analysis of hygienic standard of vegetables and fruits[S].Beijing:Standards Press of China,2004.中华人民共和国卫生部,中国国家标准化管理委员会.GB/T5009.38-2003蔬菜、水果卫生标准的分析方法[S].北京:中国标准出版社,2004.
[25]HU J B,MA S W,LI J W,et al.Genetic diversity of foreign melon(Cucumis melo)germplasm resources by morphological characters[J].Chin Bull Bot,2013,48(1):42-51.doi:10.3724/SP.J.1259.2013.00042.胡建斌,马双武,李建吾,等.国外甜瓜种质资源形态性状遗传多样性分析[J].植物学报,2013,48(1):42-51.doi:10.3724/SP.J.1259.2013.00042.
[26]TONG Y Q,LIU X N.Establishment of several quantitative characters in selective breeding of Prunus sibirica Linn.in northwest drought region[J].N Hort,2009(2):29-31.仝玉琴,刘小宁.西北干旱地区西伯利亚杏选育若干数量性状的确定[J].北方园艺,2009(2):29-31.
[27]Institute of Nutrition and Food Safety,Chinese Center for Disease Control and Prevention.China Food Composition 2002[M].Beijing:Peking University Medical Press,2002.中国疾病预防控制中心营养与食品安全所.中国食物成分表2002[M].北京:北京医科大学出版社,2002.
[28]WANG D,DAI G Z,YANG J,et al.A comparative study on standards of selenium content in selenium-enriched products[J].Hubei Agric Sci,2018,57(15):11-15.doi:10.14088/j.cnki.issn0439-8114.2018.15.002.汪丹,戴光忠,杨军,等.富硒产品硒含量标准对比研究[J].湖北农业科学,2018,57(15):11-15.doi:10.14088/j.cnki.issn0439-8114.2018.15.002.
[29]XU R,ZHANG Y N,LIN Z X,et al.Analysis in agronomic characters of ornamental pepper germplasm resources[J].Acta Agric Zhejiang,2018,30(11):1886-1892.doi:10.3969/j.issn.1004-1524.2018.11.11.徐睿,张雅楠,林子翔,等.观赏辣椒种质资源农艺性状遗传多样性关联分析[J].浙江农业学报,2018,30(11):1886-1892.doi:10.3969/j.issn.1004-1524.2018.11.11.
[30]GAO H X,SUN J L,ZHANG F Y,et al.Identification and evaluation of twenty-four characteristics of Momordica charantia L.[J].N Hort,2018(15):13-17.doi:10.11937/bfyy.20180449.高红霞,孙江乐,张凤银,等.苦瓜品种资源二十四个性状的鉴定评价[J].北方园艺,2018(15):13-17.doi:10.11937/bfyy.20180449.