摘要
目的:分析miR-21的功能及其在结肠癌中的临床意义。方法:采用miRWalk在线工具预测和筛选miR-21的靶基因,并使用DAVID分析靶基因的功能和信号通路;检测结肠癌患者和健康对照人群血清以及癌组织和癌旁组织标本的miR-21的表达水平;使用TCGA数据库中结肠癌的数据,分析miR-21表达水平与临床病理参数的关系以及与患者预后的关系。结果:共筛选出429个miR-21的靶基因,靶基因功能分析提示这些基因主要参与了正性调节代谢过程、蛋白结合等。结肠癌患者血清miR-21表达水平显著高于健康对照人群,差异有统计学意义(P <0. 05)。TCGA数据显示,结肠癌组织中的miR-21表达水平与肿瘤的临床分期、肿瘤侵袭程度、淋巴结转移分期、远处转移分期无显著差异(P> 0. 05)。生存分析显示组织中高表达miR-21的患者其生存时间较低表达者短(P <0. 05)。结论:miR-21调控多个肿瘤发生相关的基因,在结肠癌发生过程中具有重要的调控作用。结肠癌患者miR-21表达水平显著高于健康人群,且miR-21高表达的患者预后差。
Objective: To analyze the function of miR-21 and the clinical significance in colon cancer. Methods:The target genes of miR-21 were screened by the miRWalk online tool,and the gene function and signal pathways were analyzed by DAVID tool. The serum levels of miR-21 were tested and compared in colon cancer patients and healthy controls. The data of colon cancer was download from TCGA database,and the relation of miR-21 with clinicopathological parameters were analyzed,the relation of miR-21 and prognosis of colon cancer patients was also analyzed. Results: 429 validated target genes of miR-21 were screened. These target genes were involving the positive regulation of metabolic process,protein binding. The serum levels of miR-21 were significantly increased in colon patients compared with that in healthy controls( P < 0. 05). The data of TCGA revealed that the expression of miR-21 in colon cancer tissue has not significant difference in clinical stage,tumor invasive,lymph node metastasis,distant metastasis of colon cancer( P > 0. 05). Survival analysis showed that high expression of miR-21 was associated with shorter survival time compared with low expression( P < 0. 05). Conclusion: miR-21 participate in the process of colon cancer by regulating several target genes. The expression of miR-21 was increased in colon patients compared with that in healthy controls,and high expression of miR-21 indicates a poor prognosis of colon cancer patients.
引文
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