老年宫颈鳞癌患者HPV16/18感染与癌组织PDGFR-α和PDGFR-β蛋白表达的关系
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摘要
目的:在老年宫颈鳞癌患者中探索HPV16/18感染与癌组织血小板衍生生长因子受体α(platelet-derived growth factor receptor-alpha,PDGFR-α)和血小板衍生生长因子受体β(platelet-derived growth factor receptor-alpha,PDGFR-β)蛋白表达的关系以及三者之间的相互关系。方法:选取老年宫颈鳞癌患者75例、正常子宫颈组织32例,分别采用原位杂交技术检测HPV16/18感染,用免疫组织化学染色检测PDGFR-α和PDGFR-β蛋白。结果:老年宫颈鳞癌患者中HPV16/18感染率高达69.33%(52/75),而正常对照组为18.75%(6/32),老年宫颈鳞癌患者HPV16/18的感染率均显著高于对照组(P<0.05)。在对照组中检测到1例PDGFR-α蛋白的表达,表达阳性率3.13%,而老年宫颈鳞癌患者组织中检测到59例PDGFR-α蛋白的阳性表达,表达阳性率78.67%,差异有统计学意义(P <0.05);PDGFR-α蛋白的阳性表达率与宫颈鳞癌患者临床分期有关(P <0.05);在对照组中PDGFR-β蛋白阳性表达率较低为6.25%,而宫颈鳞癌患者组织中PDGFR-β蛋白表达率较高为62.67%,差异有统计学意义(P<0.05); PDGFR-β蛋白表达量与宫颈鳞临床分期(P<0.05);在72例宫颈鳞癌组织中,HPV16/18感染阳性患者共有47例,表达阳性率62.67%,其中PDGFR-α蛋白阳性有45例,表达阳性率95.74%,PDGFR-β蛋白阳性有40例,表达阳性率85.11%,显著高于未感染HPV16/18的宫颈鳞癌患者,差异有统计学意义(P<0.05),Spearman相关性分析发现PDGFR-α与PDGFR-β蛋白的表达与宫颈癌患者HPV16/18感染呈显著正相关(rs=0.428,P <0.05; rs=0.562,P <0.05);此外,在宫颈癌患者中,PDGFR-α与PDGFR-β蛋白表达水平也呈显著正相关(rs=0.639,P <0.05)。结论:老年宫颈鳞癌的发生与HPV16/18感染关系密切,在HPV16/18感染的宫颈鳞癌组织中PDGFR-α与PDGFR-β蛋白表达水平显著增高,并且在宫颈鳞癌发生、发展中起着协同作用,共同促进宫颈鳞癌的发展。
Objective: The relationship between the expression of HPV16/18 infection and the expression of platelet derived growth factor receptor alpha( platelet-derived growth factor receptor-alpha,PDGFRa) and platelet derived growth factor receptor beta( platelet-derived growth factor receptor-alpha,PDGFRbeta) in the elderly patients with squamous cell carcinoma of the cervix and the expression of platelet derived growth factor receptor beta( platelet-derived growth factor receptor-alpha,PDGFR-beta) and the relationship between the three The relationship between each other.Methods: 75 cases of senile cervical squamous cell carcinoma and 32 of normal cervical tissue were selected. In situ hybridization was used to detect HPV16/18 infection,and PDGFR-A and PDGFR-beta protein were detected by immunohistochemistry. Results: The infection rates of HPV16/18 in cervical squamous cell carcinoma group and control group were 69.33%( 52/75) and 18.75%( 6/32) respectively.The infection rate of HPV16/18 in elderly patients with cervical squamous cell carcinoma was significantly higher than that in the control group( P <0. 05). One case of PDGFR-α protein expression was detected in the control group,Positive expression rate 3.13%. In the elderly patients with cervical squamous cell carcinoma,59 cases of PDGFR-α protein were detected positively,Positive expression rat 78.67%,and the difference was statistically significant.( P <0.05); The positive expression rate of PDGFR-α protein was related to the clinical stage of cervical squamous cell carcinoma patients( P <0.05);The positive expression rate of PDGFR-β protein was lower in the control group was 6.25%,and the cervical squamous cell carcinoma patients The expression rate of PDGFR-β protein in tissues was 62.67%,and the difference was statistically significant( P <0.05). The expression of PDGFR-β protein was correlated with the clinical stage of cervical scales( P < 0.05); Among 72 cases of cervical squamous cell carcinoma,47 cases( Positive expression rat 62.67%) were HPV16/18 positive,45 cases( Positive expression rat 95.74%) were PDGFR-α protein positive,40 cases( Positive expression rat 85.11%) were PDGFR-β protein positive.The number of HPV16/18 positive cervical squamous cell carcinoma patients was significantly higher than that of non-HPV16/18 positive cervical squamous cell carcinoma patients( P < 0.05).Spearman correlation analysis showed that the expression of PDGFR-α and PDGFR-β protein was positively correlated with HPV16/18 infection in patients with cervical cancer( rs = 0.428,P < 0.05; rs = 0.562,P < 0.05). PDGFR-α was positively correlated with the expression of PDGFR-β protein in cervical cancer patients( rs = 0.639,P <0.05).Conclusions: Senile cervical squamous cell carcinoma is closely related to HPV16/18 infection. The expression of PDGFR-α and PDGFR-β protein in HPV16/18 infected cervical squamous cell carcinoma is significantly increased.It plays a synergistic role in the occurrence and development of cervical squamous cell carcinoma.
Objective: The relationship between the expression of HPV16/18 infection and the expression of platelet derived growth factor receptor alpha( platelet-derived growth factor receptor-alpha,PDGFRa) and platelet derived growth factor receptor beta( platelet-derived growth factor receptor-alpha,PDGFRbeta) in the elderly patients with squamous cell carcinoma of the cervix and the expression of platelet derived growth factor receptor beta( platelet-derived growth factor receptor-alpha,PDGFR-beta) and the relationship between the three The relationship between each other.Methods: 75 cases of senile cervical squamous cell carcinoma and 32 of normal cervical tissue were selected. In situ hybridization was used to detect HPV16/18 infection,and PDGFR-A and PDGFR-beta protein were detected by immunohistochemistry. Results: The infection rates of HPV16/18 in cervical squamous cell carcinoma group and control group were 69.33%( 52/75) and 18.75%( 6/32) respectively.The infection rate of HPV16/18 in elderly patients with cervical squamous cell carcinoma was significantly higher than that in the control group( P <0. 05). One case of PDGFR-α protein expression was detected in the control group,Positive expression rate 3.13%. In the elderly patients with cervical squamous cell carcinoma,59 cases of PDGFR-α protein were detected positively,Positive expression rat 78.67%,and the difference was statistically significant.( P <0.05); The positive expression rate of PDGFR-α protein was related to the clinical stage of cervical squamous cell carcinoma patients( P <0.05);The positive expression rate of PDGFR-β protein was lower in the control group was 6.25%,and the cervical squamous cell carcinoma patients The expression rate of PDGFR-β protein in tissues was 62.67%,and the difference was statistically significant( P <0.05). The expression of PDGFR-β protein was correlated with the clinical stage of cervical scales( P < 0.05); Among 72 cases of cervical squamous cell carcinoma,47 cases( Positive expression rat 62.67%) were HPV16/18 positive,45 cases( Positive expression rat 95.74%) were PDGFR-α protein positive,40 cases( Positive expression rat 85.11%) were PDGFR-β protein positive.The number of HPV16/18 positive cervical squamous cell carcinoma patients was significantly higher than that of non-HPV16/18 positive cervical squamous cell carcinoma patients( P < 0.05).Spearman correlation analysis showed that the expression of PDGFR-α and PDGFR-β protein was positively correlated with HPV16/18 infection in patients with cervical cancer( rs = 0.428,P < 0.05; rs = 0.562,P < 0.05). PDGFR-α was positively correlated with the expression of PDGFR-β protein in cervical cancer patients( rs = 0.639,P <0.05).Conclusions: Senile cervical squamous cell carcinoma is closely related to HPV16/18 infection. The expression of PDGFR-α and PDGFR-β protein in HPV16/18 infected cervical squamous cell carcinoma is significantly increased.It plays a synergistic role in the occurrence and development of cervical squamous cell carcinoma.
引文
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[4]徐衍杰,郑智,刘鹏,等.胃癌组织中CAP70和血小板源性生长因子受体β的表达及其与预后的关系[J].中华普通外科杂志,2015,30(10):813~814.
[5] Bhogireddy V,Roston A,Chor J,et al. Cervical intraepithelial neoplasia and cancer in women 35 years and older.[J]. Journal of Lower Genital Tract Disease,2016,18(1):41~45.
[6]邱笑飞,林敏,姚招男,等.宫颈上皮内瘤变及宫颈癌I期患者高危型人乳头瘤病毒的变化情况[J].中华医院感染学杂志,2017,27(7):1597~1600.
[7]唐婉容,李丽华,喻洁,等.TGF-β1对口腔鳞癌相关成纤维细胞FAP表达的影响[J].安徽医科大学学报,2017,52(9):1293~1297.