低温等离子体制备天然功能性多糖
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摘要
天然多糖由于具有天然可再生、价格低廉、生物相容好等优点,受到广泛关注。该文选择具有代表性的羧甲基纤维素钠和海藻酸钠作为研究对象,探讨了低温等离子体技术应用于多糖改性的可行性。通过系统地研究等离子体处理对这两种多糖溶液黏度、pH值、菌落总数及1,1-二苯基-2-三硝基苯肼清除率等性能的影响,并在此基础上,分析等离子体处理对两种多糖结构和性质的影响差异及相关机制。实验结果表明,处理后的多糖具备了一定的抗菌及抗氧化性能。主要机制为:等离子体处理过程中产生的能量,使多糖内部的分子键断裂;这个过程中,也引入了含氧、含氮等活性官能团,与断键之后的多糖稳定结合,从而赋予其相应的功能。相较于传统的酸、碱和酶提取法制备功能性多糖,低温等离子体更为稳定、高效且没有任何化学试剂污染。因此,低温等离子体处理为功能性多糖的制备提供了新的方法。
Polysaccharides have attracted wide attention due to their renewable, biocompatibility and low cost. The objective of this research was to study the effect of cold plasma treatment on the properties of sodium carboxymethyl cellulose and sodium alginate. The viscosity, pH, total viable colonies and 1,1-diphenyl-2-picrylhydrazyl values were determined. Besides, the structures and mechanisms of sodium carboxymethyl cellulose and sodium alginate were also discussed. Results indicate that sodium carboxymethyl cellulose and sodium alginate modified with cold plasma treatment obtained antimicrobial and antioxidant ability. This is mainly attributes to the energy produced during plasma treatment breaks the molecular bonds within polysaccharides, meanwhile some active groups containing oxygen and nitrogen are introduced. Then the active groups could combine with the broken molecular band, lead to some functional properties. Compared with some traditional methods such as acid and alkali exaction, cold plasma treatment possesses stability and high efficiency, without any chemical pollution. Thus, cold plasma treatment provides a new method for the preparation of functional polysaccharides.
Polysaccharides have attracted wide attention due to their renewable, biocompatibility and low cost. The objective of this research was to study the effect of cold plasma treatment on the properties of sodium carboxymethyl cellulose and sodium alginate. The viscosity, pH, total viable colonies and 1,1-diphenyl-2-picrylhydrazyl values were determined. Besides, the structures and mechanisms of sodium carboxymethyl cellulose and sodium alginate were also discussed. Results indicate that sodium carboxymethyl cellulose and sodium alginate modified with cold plasma treatment obtained antimicrobial and antioxidant ability. This is mainly attributes to the energy produced during plasma treatment breaks the molecular bonds within polysaccharides, meanwhile some active groups containing oxygen and nitrogen are introduced. Then the active groups could combine with the broken molecular band, lead to some functional properties. Compared with some traditional methods such as acid and alkali exaction, cold plasma treatment possesses stability and high efficiency, without any chemical pollution. Thus, cold plasma treatment provides a new method for the preparation of functional polysaccharides.
引文
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[13]翟佳慧,孙庆申,刘春光,等.改性壳聚糖的制备及抑菌效果评价[J].黑龙江大学自然科学学报,2010,27(6):778-782.
[14]马凤鸣,王振宇,赵海田,等.脉冲放电等离子体技术提取黑木耳多糖[J].农业工程学报,2010(S1):363-368.
[15]Cheng HH,Chen SS,Yoshizuka K,et al.Degradation of phenolic compounds in water by non-thermal plasma treatment[J].Journal of Water Chemistry and Technology,2012,34(4):179-189.
[2]孙业全,王秀春,陈常胜,等.羧甲基纤维素钠在卵巢癌介入治疗中的应用[J].山东医药,2006,46(23):21-22.
[3]李飞飞,万丹丹,严孝强.二甲双胍-羧甲基纤维素钠聚电解质聚集体作为药物缓释载体的研究[J].北方药学,2015,12(1):108-109.
[4]盘茂东,李嘉诚,林强,等.海藻酸钠在药物控释中的应用[J].中国药业,2008,17(19):3-5.
[5]张莉华,李科,卜方方.海藻酸钠在药物制剂中的应用进展[J].中南药学,2016,14(1):52-56.
[6]熊青.大气压低温等离子体射流的研究[D].武汉:华中科技大学,2013.
[7]姚骏,张弘,郭森,等.海带的生物活性及系列产品开发研究进展[J].食品研究与开发,2018,39(8):198-202.
[8]董诗竹.海带低分子量岩藻聚糖硫酸酯的制备及其抗血栓活性研究[D].青岛:中国海洋大学,2011.
[9]李汶潓,李亚鹤,徐年军,等.壳聚糖酶法降解工艺优化及其产物的抗氧化活性[J].中国食品学报,2017,17(11):121-130.
[10]Chokradjaroen C,Rujiravanit R,Watthanaphanit A,et al.Enhanced degradation of chitosan by applying plasma treatment in combination with oxidizing agents for potential use as an anticancer agent[J].Carbohydrate Polymers,2017,167:1-11.
[11]Ekezie FGC,Sun DW,Cheng JH.A review on recent advances in cold plasma technology for the food industry:current applications and future trends[J].Trends in Food Science&Technology,2017,69:46-58.
[12]马凤鸣.壳聚糖的等离子体降解动力学模型及结构表征与生物活性[D].哈尔滨:哈尔滨工业大学,2013.
[13]翟佳慧,孙庆申,刘春光,等.改性壳聚糖的制备及抑菌效果评价[J].黑龙江大学自然科学学报,2010,27(6):778-782.
[14]马凤鸣,王振宇,赵海田,等.脉冲放电等离子体技术提取黑木耳多糖[J].农业工程学报,2010(S1):363-368.
[15]Cheng HH,Chen SS,Yoshizuka K,et al.Degradation of phenolic compounds in water by non-thermal plasma treatment[J].Journal of Water Chemistry and Technology,2012,34(4):179-189.