丝胶对2型糖尿病大鼠肝脏IR的调节作用
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摘要
目的:研究丝胶对2型糖尿病大鼠肝脏IR的调节作用。方法:36只SPF级雄性SD大鼠随机分为正常组、模型组和实验组,每组12只。采用高脂高糖饲料喂养+腹腔注射STZ的方法建立2型糖尿病大鼠模型。成模后,给予实验组大鼠丝胶灌胃35d。采用葡萄糖氧化酶法检测各组大鼠空腹血糖;Real Time Q-PCR法检测各组大鼠肝脏IR mRNA的表达。结果:与模型组大鼠相比,实验组大鼠血糖明显降低,IR mRNA的表达明显升高(P<0.05)。结论:丝胶可能通过上调肝脏IR的表达来增强胰岛素信号的转导效应,进而起到降低血糖的作用。
Objective: To investigate th regulatory effects of sericin on liver insulin receptor(IR) in type 2 diabetic rats. Methods: Thirty-six SPF male rats were randomly divided into three groups(normal group, model group and experimental group), with 12 rats in each group. The rat model of type 2 diabetes mellitus was induced by high-fat, high-sugar feeding and intraperitoneal injection of streptozotocin. After modeling, the rats were given sericin by gavaging for 35 days in the experimental group. The glucose oxidase method was used to detect the fasting blood glucose of rats in each group. Real Time Q-PCR was used to detect the expression of IR mRNA in liver. Results: Compared with the rats in model group, the blood glucose of rats in experimental group was significantly reduced, and the expression of liver IR of rats in experimental group increased significantly(P< 0.05). Conclusions: Sericin may enhance the transduction effects of insulin signal by up regulating the expression of IR in liver of diabetes mellitus rats, thus reducing the blood glucose.
Objective: To investigate th regulatory effects of sericin on liver insulin receptor(IR) in type 2 diabetic rats. Methods: Thirty-six SPF male rats were randomly divided into three groups(normal group, model group and experimental group), with 12 rats in each group. The rat model of type 2 diabetes mellitus was induced by high-fat, high-sugar feeding and intraperitoneal injection of streptozotocin. After modeling, the rats were given sericin by gavaging for 35 days in the experimental group. The glucose oxidase method was used to detect the fasting blood glucose of rats in each group. Real Time Q-PCR was used to detect the expression of IR mRNA in liver. Results: Compared with the rats in model group, the blood glucose of rats in experimental group was significantly reduced, and the expression of liver IR of rats in experimental group increased significantly(P< 0.05). Conclusions: Sericin may enhance the transduction effects of insulin signal by up regulating the expression of IR in liver of diabetes mellitus rats, thus reducing the blood glucose.
引文
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[9]钱俊文,柴可夫.加减抵当汤对胰岛素抵抗大鼠肝脏胰岛素受体基因表达的影响[J].浙江中医药大学学报,2007,31(3):295-298.
[2]戴悦萱,李月春,梁芙茹.PI3K/AKT/GSK3β通路影响糖代谢与认知过程的研究进展[J].中西医结合心血管病杂志,2018,6(6):28-29.
[3]师林,杨玉彬,黄颖娟,等.健脾化湿方对脾虚痰湿型肥胖2型糖尿病胰岛素抵抗大鼠骨骼肌PI3K/Akt通路的影响[J].中华中医药杂志,2018,33(2):530-534.
[4]付秀美,钟美蓉,付文亮,等.丝胶对2型糖尿病大鼠血糖和血脂的影响[J].中国老年学杂志,2011,31(1):103-105.
[5]Gao Y,Zhang M,Wu T,et al.Effects of D-Pinitol on Insulin Resistance through the PI3K/Akt Signaling Pathway in Type 2 Diabetes Mellitus Rats[J].J Agric Food Chem,2015,63(26):6019-6026.
[6]赵海燕,张长宁,黎丽萍,等.维格列汀联合胰岛素治疗2型糖尿病的临床研究[J].药物与临床,2018,15(3):60-64.
[7]王维维,朱文静.丝胶蛋白在组织工程中的应用概况[J].中国药业,2012,21(10):14-16.
[8]张敏,何继瑞.邻苯二甲酸二(2-乙基)己酯与胰岛素抵抗的研究进展[J].中国全科医学,2018,21(8):993-997.
[9]钱俊文,柴可夫.加减抵当汤对胰岛素抵抗大鼠肝脏胰岛素受体基因表达的影响[J].浙江中医药大学学报,2007,31(3):295-298.