济川煎对结肠慢传输型便秘模型大鼠血浆SP、肠组织ICC与肠推动力等因素影响的研究
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摘要
目的研究济川煎对结肠慢传输型便秘模型大鼠血浆P物质(SP)、肠组织Cajal间质细胞(ICC)及结肠推动力等因素的影响,探讨济川煎治疗慢传输型便秘的机制,并为临床选择治疗剂量提供依据。方法建立慢传输型便秘大鼠模型,分别予小、中、高剂量济川煎液灌胃大鼠,同时设立模型组及空白对照组,测定各组大鼠粪便湿重、大鼠体重、血浆SP含量及肠组织c-kit mRNA含量等指标。结果济川煎各剂量组大鼠粪便湿重均高于模型组,小剂量组与模型组比较差异有统计学意义(P<0.05);济川煎各剂量组大鼠体重均低于模型组,中剂量组与模型组比较差异有统计学意义(P<0.05);济川煎各剂量组大鼠首次排便时间均短于模型组(P<0.05),在一定范围内呈剂量依赖性;济川煎各剂量组大鼠平均肠推动率大于模型组,中剂量组、高剂量组与模型组比较差异均有统计学意义(P<0.05,P<0.01);血浆SP含量模型组低于济川煎各剂量组,高剂量组与模型组比较差异有统计学意义(P<0.05);大鼠肠组织中c-kit mRNA含量模型组低于济川煎各剂量组,中剂量组、高剂量组与模型组比较差异均有统计学意义(P<0.05,P<0.01)。结论济川煎可能通过影响大鼠血浆SP、肠组织ICC含量及粪便含水量等因素,促进大鼠肠蠕动,改善慢传输型便秘模型大鼠便秘状况,且在本试验用药范围内呈剂量依赖性。
Objective To study the effects of Jichuan Decoction on plasma substance P( SP),interstitial cells of Cajal( ICC),colonial motility of model rats with slow transit constipation,to speculate the mechanism of the effectiveness of this drug on slow transit constipation,in order to provide appropriate dosage in clinical treatment. Methods The model of slow transit constipation rats was established and were given small,medium and high doses of Jichuan Decoction respectively,meanwhile,the model group and the blank control group were also established,the stool wet weight,body weight,the contents of plasma SP and intestinal tissue c-kit mRNA were determined,and the results were statistically analyzed. Results The average value of stool wet weight of three Jichuan Decoction groups was higher than that of the constipation model group,and that of the small dose group was statistically different from that of the model group( P < 0. 05). The mean weight of rats of Jichuan decoction groups was lower than that of the model group,and there was a statistically significant difference between the medium dose group and the model group( P < 0. 05). The first defecation time of the three groups was shorter than that of the model group( P < 0. 05). The intestinal motility of three Jichuan Decoction groups was larger than that of model group and there was a statistically significant difference between the medium dose group and the model group( P < 0. 05). There was a statistical difference between the high-dose group and model group( P < 0. 01). Plasma SP content of model group was lower than that of the three groups of Jichuan Decoction,There was a statistical difference between the high-dose group and model group( P < 0. 05). The c-kit mRNA content of intestinal tissues of model group was lower than that of the three groups of Jichuan Decoction,there was a significant difference between the model group and the medium-dose group( P < 0. 05) and there was a highly significant difference between the model group and the high-dose group( P < 0. 01). Conclusion Jichuan Decoction may improve intestinal peristalsis and constipation of rats with STC by affecting SP,ICC content and fecal water content of rats,and theadministration of drugs are dosage dependent within the scope of drug dosage in the test.
Objective To study the effects of Jichuan Decoction on plasma substance P( SP),interstitial cells of Cajal( ICC),colonial motility of model rats with slow transit constipation,to speculate the mechanism of the effectiveness of this drug on slow transit constipation,in order to provide appropriate dosage in clinical treatment. Methods The model of slow transit constipation rats was established and were given small,medium and high doses of Jichuan Decoction respectively,meanwhile,the model group and the blank control group were also established,the stool wet weight,body weight,the contents of plasma SP and intestinal tissue c-kit mRNA were determined,and the results were statistically analyzed. Results The average value of stool wet weight of three Jichuan Decoction groups was higher than that of the constipation model group,and that of the small dose group was statistically different from that of the model group( P < 0. 05). The mean weight of rats of Jichuan decoction groups was lower than that of the model group,and there was a statistically significant difference between the medium dose group and the model group( P < 0. 05). The first defecation time of the three groups was shorter than that of the model group( P < 0. 05). The intestinal motility of three Jichuan Decoction groups was larger than that of model group and there was a statistically significant difference between the medium dose group and the model group( P < 0. 05). There was a statistical difference between the high-dose group and model group( P < 0. 01). Plasma SP content of model group was lower than that of the three groups of Jichuan Decoction,There was a statistical difference between the high-dose group and model group( P < 0. 05). The c-kit mRNA content of intestinal tissues of model group was lower than that of the three groups of Jichuan Decoction,there was a significant difference between the model group and the medium-dose group( P < 0. 05) and there was a highly significant difference between the model group and the high-dose group( P < 0. 01). Conclusion Jichuan Decoction may improve intestinal peristalsis and constipation of rats with STC by affecting SP,ICC content and fecal water content of rats,and theadministration of drugs are dosage dependent within the scope of drug dosage in the test.
引文
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[7]金伟,崔雅飞,杨爱龙,等.黄芪润肠丸对小鼠慢传输性便秘结肠肌神经丛VIP及P物质的影响[J].中医药信息,2009,26(5):131-132.
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[11]Lyford GL,He CL,Soffer E,et al.Pan-colonic decrease in interstitial cells of Cajal in patients with slow transit constipation[J].Gut,2002,51(4):496-501.
[12]包云光,舒小莉,李小兵,等.肠神经系统递质和Cajal间质细胞在大鼠慢传输型便秘中的作用[J].中国当代儿科杂志,2009,11(6):481-485.
[13]于彬,梅峰,童卫东,等.慢传输便秘患者结肠壁内Cajal间质细胞的形态学研究[J].消化外科,2004,3(3):185-189.
[14]Ward SM,Sanders KM.Physiology and pathophysiology of the intersitial cell of Cajal:from bench to bedside.Am JPhysical Gastrointest Liner Physiol,2001,281(3):G602-G611.
[15]郑倩.慢传输型便秘大鼠胃肠道c-kit基因的表达[D].合肥:安徽医科大学,2013.
[2]杨丽.济川煎加减治疗功能性便秘对照观察[J].实用中医内科杂志,2012,26(3):40-41.
[3]包云光,李小兵,赵婵.复方苯乙哌啶对大鼠肠传输功能、病理和神经递质的远期影响[J].浙江医学教育,2012,11(2):43-45,49.
[4]何春梅,陆金根,曹永清.益气开秘方对结肠慢输型便秘大鼠肠动力和肠神经肽的影响[J].中西医结合学报,2007,5(2):160-164.
[5]闫学堂.麻子仁丸、济川煎方治疗慢传输型便秘的理论及实验研究[D].北京:北京中医药大学,2010.
[6]朱振.加味济川煎治疗功能性慢传输型便秘的临床观察[D].武汉:湖北中医药大学,2010.
[7]金伟,崔雅飞,杨爱龙,等.黄芪润肠丸对小鼠慢传输性便秘结肠肌神经丛VIP及P物质的影响[J].中医药信息,2009,26(5):131-132.
[8]Takayama I,Horiguchi K,Daigo Y,et al.The interstitial cells of Cajal and a gastroenteric pacemaker system[J].Arch Histol Cytol,2002,65(1):1-26.
[9]Mitsui R,Komuro T.Direct and indirect innervation of smooth muscle cells of rat stomach with special reference to the interstitial cells of Cajal[J].Cell Tissue Res,2002,309(2):219-227.
[10]陈立军,张廷模,彭成.大黄不同炮制品对热结便秘模型大鼠结肠c-kitmRNA表达的影响[J].中药药理与临床,2009,25(4):37-40.
[11]Lyford GL,He CL,Soffer E,et al.Pan-colonic decrease in interstitial cells of Cajal in patients with slow transit constipation[J].Gut,2002,51(4):496-501.
[12]包云光,舒小莉,李小兵,等.肠神经系统递质和Cajal间质细胞在大鼠慢传输型便秘中的作用[J].中国当代儿科杂志,2009,11(6):481-485.
[13]于彬,梅峰,童卫东,等.慢传输便秘患者结肠壁内Cajal间质细胞的形态学研究[J].消化外科,2004,3(3):185-189.
[14]Ward SM,Sanders KM.Physiology and pathophysiology of the intersitial cell of Cajal:from bench to bedside.Am JPhysical Gastrointest Liner Physiol,2001,281(3):G602-G611.
[15]郑倩.慢传输型便秘大鼠胃肠道c-kit基因的表达[D].合肥:安徽医科大学,2013.