半夏泻心汤的工艺改进及药效研究
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摘要
半夏泻心汤出自汉代著名医家张仲景的经典古方,目前在临床上依然被广泛用于治疗胃炎胃溃疡,本研究对处方用量进行调整,并采用乙醇提取、大孔树脂纯化的方法进行工艺改进,获得4种提取组分,通过对其进行胃溃疡的抑制作用、抗炎作用、以及对幽门螺杆菌感染的治疗作用等药效研究,筛选出药效最强的分离部位,试验结果显示,采用质量分数为40%乙醇提取,并通过AB-8大孔树脂富集,5倍量树脂体积的40%乙醇洗脱得到的分离部位对抑制胃溃疡、抗炎以及杀灭幽门螺杆菌的作用最强,故提取分离该有效部位,用于治疗胃炎、胃溃疡,可最大限度的减少患者服用量,保留有效成分,为将古方制备成方便服用、质量可控、易保存的现代制剂提供技术及理论基础。
The macroporous resin was applied in the improved purification process of Banxia XiexinTang,a classical ancient prescription. Four kinds of extracting components have been obtained. With the examination on the inhibiting effect on gastrohelcosis,anti-inflammatory action and the therapeutic action on helicobacter pylori infection,the most effective part has been screen out. The results show that the separation part eluted by40% ethanol and enriched by AB-8 resin and 40% ethanol extraction solution presents superior therapeutic performances on gastritis and gastrohelcosis due to the maximum retention of effective ingredients and the minimum dosage. This improved technology provides the technical and theoretical basis for the modern pharmaceutical technology to transform the classical ancient prescriptionin to a convenient and qualitycontrollable medicine.
The macroporous resin was applied in the improved purification process of Banxia XiexinTang,a classical ancient prescription. Four kinds of extracting components have been obtained. With the examination on the inhibiting effect on gastrohelcosis,anti-inflammatory action and the therapeutic action on helicobacter pylori infection,the most effective part has been screen out. The results show that the separation part eluted by40% ethanol and enriched by AB-8 resin and 40% ethanol extraction solution presents superior therapeutic performances on gastritis and gastrohelcosis due to the maximum retention of effective ingredients and the minimum dosage. This improved technology provides the technical and theoretical basis for the modern pharmaceutical technology to transform the classical ancient prescriptionin to a convenient and qualitycontrollable medicine.
引文
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[12]赵鸣芳.半夏泻心汤的应用思路及作用机理分析[J].江苏中医药,2005,26(10):16-49.
[13]谢田.胃溃疡实验动物模型制作方法的研究进展[J].中国伤残医学,2014,22(10):309-310.
[14]谢珍连,甘广玉,罗爱月,等.大驳骨和落地生根及其配伍抗炎镇痛的实验研究[J].中国医院药学杂志,2018,38(17):1792-1795.
[15]杨行堂,张俊杰,王志荣,等.幽门螺杆菌临床分离株耐药特点分析[J].世界华人消化杂志,2007,15(9):1012-1015.
[2]胡进明.奥美拉唑、克拉霉素、阿莫西林三联疗法根除幽门螺杆菌[J].青海医药杂志,2005,32(4):9-11.
[3]梁标.奥美拉唑联合左氧氟沙星与克拉霉素三联疗法治疗慢性胃炎的疗效[J].实用中西医结合临床,2016,12(2):69-70.
[4]杨行堂,林永辉,张俊杰,等.甘草活性成分抗幽门螺杆菌实验研究[J].同济大学学报(医学版),2013,34(6):26-29.
[5]方药中,邓铁涛,李克光,等.实用中医内科学[M].上海:上海科学技术出版社,1997:215-222.
[6]万俊华,王春煦,尹晓华,等.中医治疗慢性胃炎研究进展[J].亚太传统医药,2015,11(22):49-50.
[7]尚炽昌,冀春茹,苗明三,等.仲景方药研究应用精鉴[M].北京:人民军医出版社,1999:401.
[8]张保国,刘庆芳.半夏泻心汤现代与临床应用[J].中成药,2011,33(2):318-321.
[9]张永胜,乔文慧.加味半夏泻心汤对慢性萎缩性胃炎1 000例[J].陕西中医,2008,29(1):23-24.
[10]邹平.半夏泻心汤加西药用于胃炎治疗的观察及研究[J].中国保健营养,2019,29(4):92.
[11]宋宽跃.半夏泻心汤治疗幽门螺杆菌经验探讨[J].心理医生,2018,24(33):89-90.
[12]赵鸣芳.半夏泻心汤的应用思路及作用机理分析[J].江苏中医药,2005,26(10):16-49.
[13]谢田.胃溃疡实验动物模型制作方法的研究进展[J].中国伤残医学,2014,22(10):309-310.
[14]谢珍连,甘广玉,罗爱月,等.大驳骨和落地生根及其配伍抗炎镇痛的实验研究[J].中国医院药学杂志,2018,38(17):1792-1795.
[15]杨行堂,张俊杰,王志荣,等.幽门螺杆菌临床分离株耐药特点分析[J].世界华人消化杂志,2007,15(9):1012-1015.