胡杨天然群体叶片不定芽分化性状分析
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摘要
再生是植物实现自我延存和后代繁衍的重要手段,离体叶片的不定芽分化是植物再生能力的重要体现。本研究揭示了新疆塔里木河流域胡杨群体内离体叶片不定芽分化的变异模式,分析了分化培养过程中间隔均匀的9个时间点动态性状的分布频率、变异系数和各时间点之间的表型相关系数,成功拟合了群体内87个个体的不定芽增殖曲线,对表征曲线的渐近值、增长速率和增速期持续时间三个参数在群体内作了比较和聚类分析。结果表明:(1)102个胡杨个体中,有87个分化出不定芽。(2)在第5～9个分化时间点之间,不定芽平均分化数量表现出高度相关性,相关系数介于0.89～1.00之间;第9个分化时间点表型在群体内呈极显著差异,变异系数为55.7%。(3)采用逻辑斯蒂生长曲线对群体内个体进行不定芽再生的动态拟合,决定系数r~2>0.95,渐近值、增长速率和增速期持续时间的变异系数分别为67.7%、55.4%和53.5%。(4)聚类分析显示,群体内87个个体的不定芽增殖曲线可被分为4类。上述结果揭示,胡杨天然群体内不定芽再生性状存在着丰富的变异模式,研究结果为胡杨天然群体的高解析度基因定位提供帮助,同时也为优良再生性状的种质资源利用与保护提供依据。
Regeneration is the crucial strategy for plants to achieve survive and reproduce, and the adventitious bud differentiation of detached leaves is an important embodiment of plant regeneration ability. The research revealed the variation model in natural population of Populus euphratica which was located in Tarim River Basin,Xinjiang. The distribution frequency, variation coefficient and phenotypic correlation coefficient of dynamic traits at 9 time points with uniform intervals during differentiation and culture were analyzed. The adventitious bud growth of 87 individuals in the population was successfully fitted. Propagation curve, the asymptotic value, growth rate and duration of growth period of the characterization curve were compared and clustered in the population.The results showed that 87 out of 102 genotypes could regenerate shoots. The average number of adventitious bud differentiation showed a high correlation between 5 and 9 differentiation time points, and the correlation coefficient was between 0.89 and 1.00. The ninth tim e point of differentiation was very significant, with a coefficient of variation of 55.7%. Logistic growth curve was used to simulate adventitious bud regeneration of individuals in the population. The coefficient of determination was r2>0.95, the coefficient of variation including asymptotic value,growth rate and length of linear growth duration were 66.7%, 55.4% and 53.5%, respectively. Cluster analysis showed that the adventitious bud growth curves of 87 individuals in the group could be divided into 4 categories.Above results revealed that there was abundant variation in the phenotype of bud regeneration in the narutal population, which could provide help for the high-resolution gene mapping in natural populations of Populus euphratica. Also, it could provide basis for the utilization and protection of germplasm resources with excellent regeneration characters.
Regeneration is the crucial strategy for plants to achieve survive and reproduce, and the adventitious bud differentiation of detached leaves is an important embodiment of plant regeneration ability. The research revealed the variation model in natural population of Populus euphratica which was located in Tarim River Basin,Xinjiang. The distribution frequency, variation coefficient and phenotypic correlation coefficient of dynamic traits at 9 time points with uniform intervals during differentiation and culture were analyzed. The adventitious bud growth of 87 individuals in the population was successfully fitted. Propagation curve, the asymptotic value, growth rate and duration of growth period of the characterization curve were compared and clustered in the population.The results showed that 87 out of 102 genotypes could regenerate shoots. The average number of adventitious bud differentiation showed a high correlation between 5 and 9 differentiation time points, and the correlation coefficient was between 0.89 and 1.00. The ninth tim e point of differentiation was very significant, with a coefficient of variation of 55.7%. Logistic growth curve was used to simulate adventitious bud regeneration of individuals in the population. The coefficient of determination was r2>0.95, the coefficient of variation including asymptotic value,growth rate and length of linear growth duration were 66.7%, 55.4% and 53.5%, respectively. Cluster analysis showed that the adventitious bud growth curves of 87 individuals in the group could be divided into 4 categories.Above results revealed that there was abundant variation in the phenotype of bud regeneration in the narutal population, which could provide help for the high-resolution gene mapping in natural populations of Populus euphratica. Also, it could provide basis for the utilization and protection of germplasm resources with excellent regeneration characters.
引文
Bolibok H.,an d Rakoczy-Trojanowska M.,2006,Genetic mapping of QTLs for tissue-culture response in plants,Euphytica,149(1-2):73-83
Du F.,Xu F.,Qu H.,Feng S.,Tang J.,and Wu R.L.,2013,Exploiting the transcriptome of Euphrates poplar,Populus euphratica(Salicaceae)to develop and characterize new EST-S-SR markers and construct an EST-SSR database,PLoS One,8(4):e61337
Ferreira S.,Batista D.,Serrazina S.,and Pais M.S.,2009,Morphogenesis induction and organogenic nodule differentiation in Populus euphratica,Oliv.leaf explants,Plant Cell Tissue and Organ Culture,96(1):35-43
Gao L.X.,Chen J.K.,and Yang J.,2008,Phenotypic plasticity:eco-devo and evolution,Zhiwu Fenlei Xuebao(Journal of Systematics and Evolution),46(4):441-451(高乐旋,陈家宽,杨继,2008,表型可塑性变异的生态-发育机制及其进化意义,植物分类学报,46(4):441-451)
Goldberg R.B.,1988,Plants:novel developmental processes,Science,240(4858):1460-1467
Gordon S.P.,Heisler M.G.,Reddy G.V.,Ohno C.,Das P.,and Meyerowitz E.M.,2007,Pattern formation during de novo assembly of the Arabidopsis shoot meristem,Development,134(19):3539-3548
Huang J.,Ma F.W.,Fan J.F.,Li X.G.,and Tong J.X.,2006,In vitro plant regeneration with adventitious buds of Zizyphus jujuba leaves,Xibei Zhiwu Xuebao(Acta Botanica Boreali-Occidentalia Sinica),26(5):942-948(黄建,马锋旺,樊军锋,李新岗,同金霞,2006,枣树离体叶片不定芽再生体系建立的研究,西北植物学报,26(5):942-948)
Ji Z.F.,Gao Ya.H.,Li L.,Mao S.X.,Zhao L.,Geng Q.Y.,and Wang Y.L.,2012,Phenotypic diversity of populations of Acer mono in Huoshan mountain of Shanxi at different altitude,Yuanyi Xuebao(Acta Horticulturae Sinica),39(11):2217-2228(姬志峰,高亚卉,李乐,毛思雪,赵亮,耿全英,王祎玲,2012,山西霍山五角枫不同海拔种群的表型多样性研究,园艺学报,39(11):2217-2228)
Jia Y.K.,Duan N.,Luo H.M.,Dong L.L.,Zhang H.L.,Xu J.,and Chen H.L.,2015,Growth traits of hybrids progenies of seabuckthorn and comprehensive evaluation,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),13(12):2858-2864)(贾玉奎,段娜,罗红梅,董礼龙,张鸿怿,徐军,陈海玲,2015,中国沙棘与蒙古沙棘杂种F1代生长性状分析及其综合评价,分子植物育种,13(12):2858-2864)
Luo J.X.,and Gu W.C.,2005,Study on phenotypic diversity of natural population in Picea asperata,Linye Kexue(Scientia Silver Sinicae),41(2):66-73(罗建勋,顾万春,2005,云杉天然群体表型多样性研究,林业科学,41(2):66-73)
Ma C.X.,Casella G.,and Wu R.L.,2002,Functional mapping of quantitative trait loci underlying the character process:a theoretical framework,Genetics,161(4):1751-1762
Miguel C.M.,Druart P.,and Oliveira M.M.,1996,Shoot regeneration from adventitious buds induced on juvenile and adult almond(Prunus dulcis Mill.)explants,In Vitro Cellular&Developmental Biology-Plant,32(3):148-153
Reish B.,and Bingham E.T.,1980,The genetic control of bud formation from callus cultures of diploid alfalfa,Plant Science Letters,20(1):71-77
She M.Y.,Yin G.X.,Zhao P.,Du L.P.,Zhang P.Z.,and Ye X.G.,2015,Research progress on molecular mechanism of plant regeneration from cultured tissues via organogenesis,Kexue Daobao(Science&Technology Review),33(21):91-98(佘茂云,殷桂香,赵佩,杜丽璞,张平治,叶兴国,2015,植物组织培养过程中器官发生途径再生植株分子机制研究进展,科学导报,33(21):91-98)
Sun B.B.,Liu J.,Ge Y.C.,Sheng L.H.,Chen L.Q.,Hu X.M.,Yang Z.N.,Huang H.,and Xu L.,2016,Rencent progress on plant regeneration,Kexue Tongbao(Chinese Science Bulletin),61(36):3887-3902(孙贝贝,刘杰,葛亚超,盛李宏,陈吕琴,胡小梅,杨仲南,黄海,徐麟,2016,植物再生的研究进展,科学通报,61(36):3887-3902)
Sun L.D.,Ye M.X.,Hao H.,Wang N.T.,Wang Y.Q.,Cheng T.R.,Zhang Q.X.,and Wu R.L.,2014,A model framework for identifying genes that guide the evolution of heterochrony,Mol.Biol.Evol.,8(31):2238-2247
Tian G.D.,Zhang H.P.,Kang Z.H.,Zhao C.P.,Han M.Y.,Liu H.K.,and Tian Y.,2011,Study on shoot regeneration from leaf explants in peach,Xibei Nonglin Keji Daxue Xuebao(Journal of Northwest A&F University),39(2):125-132(田国栋,张荷芃,康卓慧,赵彩平,韩明玉,刘航空,田英,2011,桃叶片再生不定芽的研究,西北农林科技大学学报,39(2):125-132)
Tian S.P.,Wang Y.D.,Chen Y.C.,Han X.J.,and Zhan Z.Y.,2012,Phenotypic diversity of natural Litsea cubeba population's leaf and fruit traits,Shengtaixue Zazhi(Chinese Journal of Ecology),31(7):1665-1672(田胜平,汪阳东,陈益存,韩小娇,占志勇,2012,山苍子天然种群叶片和种实性状的表型多样性,生态学杂志,31(7):1665-1672)
Wang R.G.,Chen S.L.,Liu L.Y.,Hao Z.Y.,Weng H.J.,Li H.,Yang S.,and Duan S.,2005,Genotypic differences in antioxidative ability and salt tolerance of three poplars under sact stress,Beijing Linye Daxue Xuebao(Journal of Beijing Forestry University),27(3):46-52(王瑞刚,陈少良,刘力源,郝志勇,翁海娇,李鹤,杨爽,段杉,2005,盐胁迫下3种杨树的抗氧化能力与耐盐性研究,北京林业大学学报,27(3):46-52)
West G.B.,Brown J.H.,and Enquist B.J.,2001,A general model for ontogenetic growth,Nature,413(6856):628-631
Zhang C.Q.,Ji Z.F.,Lin L.L.,Zhao R.H.,and Wang L.Y.,2015,Phenotypic diversity of Acer mono Maxim population,Shengtai Xuebao(Acta Ecologica Sinica),35(16):5343-5352(张翠琴,姬志峰,林丽丽,赵瑞华,王祎玲,2015,五角枫种群表型多样性,生态学报,35(16):5343-5352)
Zhang M.M.,Bo W.H.,Xu F.,Li H.,Ye M.X.,Jiang L.B.,Shi C.Z.,Fu Y.R.,Zhao G.M.,Huang Y.J.,Gosik K.,Liang D.,and Wu R.L.,2017,The genetic architecture of shoot-root covariation during seedling emergence of a desert tree,Populus euphratica,Plant J.,90(5):918-928
Zhang Y.,Cao Y.F.,Huo H.L.,Tian L.M.,Dong X.G.,Qi D.,and Zhang X.S.,2016,Research on diversity of pear germplasm resources based on flowers phenotype traits,Yuanyi Xuebao(Acta Horticulturae Sinica),43(7):1245-1256(张莹,曹玉芬,霍宏亮,田路明,董星光,齐丹,张小双,2016,基于花表型性状的梨种质资源多样性研究,园艺学报,43(7):1245-1256)
Du F.,Xu F.,Qu H.,Feng S.,Tang J.,and Wu R.L.,2013,Exploiting the transcriptome of Euphrates poplar,Populus euphratica(Salicaceae)to develop and characterize new EST-S-SR markers and construct an EST-SSR database,PLoS One,8(4):e61337
Ferreira S.,Batista D.,Serrazina S.,and Pais M.S.,2009,Morphogenesis induction and organogenic nodule differentiation in Populus euphratica,Oliv.leaf explants,Plant Cell Tissue and Organ Culture,96(1):35-43
Gao L.X.,Chen J.K.,and Yang J.,2008,Phenotypic plasticity:eco-devo and evolution,Zhiwu Fenlei Xuebao(Journal of Systematics and Evolution),46(4):441-451(高乐旋,陈家宽,杨继,2008,表型可塑性变异的生态-发育机制及其进化意义,植物分类学报,46(4):441-451)
Goldberg R.B.,1988,Plants:novel developmental processes,Science,240(4858):1460-1467
Gordon S.P.,Heisler M.G.,Reddy G.V.,Ohno C.,Das P.,and Meyerowitz E.M.,2007,Pattern formation during de novo assembly of the Arabidopsis shoot meristem,Development,134(19):3539-3548
Huang J.,Ma F.W.,Fan J.F.,Li X.G.,and Tong J.X.,2006,In vitro plant regeneration with adventitious buds of Zizyphus jujuba leaves,Xibei Zhiwu Xuebao(Acta Botanica Boreali-Occidentalia Sinica),26(5):942-948(黄建,马锋旺,樊军锋,李新岗,同金霞,2006,枣树离体叶片不定芽再生体系建立的研究,西北植物学报,26(5):942-948)
Ji Z.F.,Gao Ya.H.,Li L.,Mao S.X.,Zhao L.,Geng Q.Y.,and Wang Y.L.,2012,Phenotypic diversity of populations of Acer mono in Huoshan mountain of Shanxi at different altitude,Yuanyi Xuebao(Acta Horticulturae Sinica),39(11):2217-2228(姬志峰,高亚卉,李乐,毛思雪,赵亮,耿全英,王祎玲,2012,山西霍山五角枫不同海拔种群的表型多样性研究,园艺学报,39(11):2217-2228)
Jia Y.K.,Duan N.,Luo H.M.,Dong L.L.,Zhang H.L.,Xu J.,and Chen H.L.,2015,Growth traits of hybrids progenies of seabuckthorn and comprehensive evaluation,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),13(12):2858-2864)(贾玉奎,段娜,罗红梅,董礼龙,张鸿怿,徐军,陈海玲,2015,中国沙棘与蒙古沙棘杂种F1代生长性状分析及其综合评价,分子植物育种,13(12):2858-2864)
Luo J.X.,and Gu W.C.,2005,Study on phenotypic diversity of natural population in Picea asperata,Linye Kexue(Scientia Silver Sinicae),41(2):66-73(罗建勋,顾万春,2005,云杉天然群体表型多样性研究,林业科学,41(2):66-73)
Ma C.X.,Casella G.,and Wu R.L.,2002,Functional mapping of quantitative trait loci underlying the character process:a theoretical framework,Genetics,161(4):1751-1762
Miguel C.M.,Druart P.,and Oliveira M.M.,1996,Shoot regeneration from adventitious buds induced on juvenile and adult almond(Prunus dulcis Mill.)explants,In Vitro Cellular&Developmental Biology-Plant,32(3):148-153
Reish B.,and Bingham E.T.,1980,The genetic control of bud formation from callus cultures of diploid alfalfa,Plant Science Letters,20(1):71-77
She M.Y.,Yin G.X.,Zhao P.,Du L.P.,Zhang P.Z.,and Ye X.G.,2015,Research progress on molecular mechanism of plant regeneration from cultured tissues via organogenesis,Kexue Daobao(Science&Technology Review),33(21):91-98(佘茂云,殷桂香,赵佩,杜丽璞,张平治,叶兴国,2015,植物组织培养过程中器官发生途径再生植株分子机制研究进展,科学导报,33(21):91-98)
Sun B.B.,Liu J.,Ge Y.C.,Sheng L.H.,Chen L.Q.,Hu X.M.,Yang Z.N.,Huang H.,and Xu L.,2016,Rencent progress on plant regeneration,Kexue Tongbao(Chinese Science Bulletin),61(36):3887-3902(孙贝贝,刘杰,葛亚超,盛李宏,陈吕琴,胡小梅,杨仲南,黄海,徐麟,2016,植物再生的研究进展,科学通报,61(36):3887-3902)
Sun L.D.,Ye M.X.,Hao H.,Wang N.T.,Wang Y.Q.,Cheng T.R.,Zhang Q.X.,and Wu R.L.,2014,A model framework for identifying genes that guide the evolution of heterochrony,Mol.Biol.Evol.,8(31):2238-2247
Tian G.D.,Zhang H.P.,Kang Z.H.,Zhao C.P.,Han M.Y.,Liu H.K.,and Tian Y.,2011,Study on shoot regeneration from leaf explants in peach,Xibei Nonglin Keji Daxue Xuebao(Journal of Northwest A&F University),39(2):125-132(田国栋,张荷芃,康卓慧,赵彩平,韩明玉,刘航空,田英,2011,桃叶片再生不定芽的研究,西北农林科技大学学报,39(2):125-132)
Tian S.P.,Wang Y.D.,Chen Y.C.,Han X.J.,and Zhan Z.Y.,2012,Phenotypic diversity of natural Litsea cubeba population's leaf and fruit traits,Shengtaixue Zazhi(Chinese Journal of Ecology),31(7):1665-1672(田胜平,汪阳东,陈益存,韩小娇,占志勇,2012,山苍子天然种群叶片和种实性状的表型多样性,生态学杂志,31(7):1665-1672)
Wang R.G.,Chen S.L.,Liu L.Y.,Hao Z.Y.,Weng H.J.,Li H.,Yang S.,and Duan S.,2005,Genotypic differences in antioxidative ability and salt tolerance of three poplars under sact stress,Beijing Linye Daxue Xuebao(Journal of Beijing Forestry University),27(3):46-52(王瑞刚,陈少良,刘力源,郝志勇,翁海娇,李鹤,杨爽,段杉,2005,盐胁迫下3种杨树的抗氧化能力与耐盐性研究,北京林业大学学报,27(3):46-52)
West G.B.,Brown J.H.,and Enquist B.J.,2001,A general model for ontogenetic growth,Nature,413(6856):628-631
Zhang C.Q.,Ji Z.F.,Lin L.L.,Zhao R.H.,and Wang L.Y.,2015,Phenotypic diversity of Acer mono Maxim population,Shengtai Xuebao(Acta Ecologica Sinica),35(16):5343-5352(张翠琴,姬志峰,林丽丽,赵瑞华,王祎玲,2015,五角枫种群表型多样性,生态学报,35(16):5343-5352)
Zhang M.M.,Bo W.H.,Xu F.,Li H.,Ye M.X.,Jiang L.B.,Shi C.Z.,Fu Y.R.,Zhao G.M.,Huang Y.J.,Gosik K.,Liang D.,and Wu R.L.,2017,The genetic architecture of shoot-root covariation during seedling emergence of a desert tree,Populus euphratica,Plant J.,90(5):918-928
Zhang Y.,Cao Y.F.,Huo H.L.,Tian L.M.,Dong X.G.,Qi D.,and Zhang X.S.,2016,Research on diversity of pear germplasm resources based on flowers phenotype traits,Yuanyi Xuebao(Acta Horticulturae Sinica),43(7):1245-1256(张莹,曹玉芬,霍宏亮,田路明,董星光,齐丹,张小双,2016,基于花表型性状的梨种质资源多样性研究,园艺学报,43(7):1245-1256)