2016年潍坊市市售动物性水产品中副溶血性弧菌和创伤弧菌污染状况研究
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摘要
目的了解潍坊市市售动物性水产品中副溶血性弧菌和创伤弧菌的污染状况及季节相关性,为食源性疾病调查研究提供依据。方法 2016年1~12月从潍坊市各大销售点采集样品210份,定性检测副溶血性弧菌和创伤弧菌,副溶血性弧菌检测毒力基因。结果副溶血性弧菌总检出率为29.05%,创伤弧菌总检出率为5.24%(P<0.01);在不同采样季节动物性水产品中副溶血性弧菌检出率差异有统计学意义(P<0.01),第一季度未检出,第三季度检出率最高,为47.69%;创伤弧菌在第三季度检出率最高,为13.85%,且在不同采样季节间差异有统计学意义(P<0.05);从210份样品中分离得到61株副溶血性弧菌,11株创伤弧菌,其中1株副溶血性弧菌携带tdh基因。结论潍坊市市售动物性水产品受到副溶血性弧菌和创伤弧菌的污染,且存在毒力株,有引起食物中毒的风险。
Objective To understand the contamination status of Vibrio parahemolyticus(Vp)and Vibrio vulnificus(Vv)in aquatic animal products from markets of Weifang city in different seasons,so as to provide the basis for the foodborne disease. Methods A total of 210 samples were collected randomly from the market of Weifang city during January-December 2016;Vpand Vv were isolated and gene tdh and trh of Vpof were amplified by PCR method. Results The total detection rate of Vpand Vv was 29.05% and 5.24% respectively with significant difference(P <0.01);The detection rates of Vpin four quarters were significantly different(P <0.01),which was the highest(47.7%)in the third-quarter while none of Vpwas detected in the first-quarter;The highest detection rate of Vv was also in the third-quarter which was 13.0%,and the detection rates of Vvin four quarters were significantly different(P <0.05),too.61 strains of Vpand 11 strains of Vv were isolated from 210 samples,and 1 Vpstrain carried virulence gene tdh. Conclusion The aquatic animal products have been contaminated by Vpand Vvin Weifang city,and a few of Vpstrains are virulence which can cause food poisoning;Relevant departments should pay more attention to the supervision of aquatic products sold on the markets.
Objective To understand the contamination status of Vibrio parahemolyticus(Vp)and Vibrio vulnificus(Vv)in aquatic animal products from markets of Weifang city in different seasons,so as to provide the basis for the foodborne disease. Methods A total of 210 samples were collected randomly from the market of Weifang city during January-December 2016;Vpand Vv were isolated and gene tdh and trh of Vpof were amplified by PCR method. Results The total detection rate of Vpand Vv was 29.05% and 5.24% respectively with significant difference(P <0.01);The detection rates of Vpin four quarters were significantly different(P <0.01),which was the highest(47.7%)in the third-quarter while none of Vpwas detected in the first-quarter;The highest detection rate of Vv was also in the third-quarter which was 13.0%,and the detection rates of Vvin four quarters were significantly different(P <0.05),too.61 strains of Vpand 11 strains of Vv were isolated from 210 samples,and 1 Vpstrain carried virulence gene tdh. Conclusion The aquatic animal products have been contaminated by Vpand Vvin Weifang city,and a few of Vpstrains are virulence which can cause food poisoning;Relevant departments should pay more attention to the supervision of aquatic products sold on the markets.
引文
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[9]何志凡,黎明,文君,等.2014年成都市动物性水产品及外环境中4种弧菌的污染情况分析[J].现代预防医学,2015,42(21):3 874-3 876.
[2]Vengadesh L,Kok-Gan C,Learn-Han L.Vibrio parahaemolyticus:a review on the pathogenesis,prevalence,and advance molecular identification techniques[J].Food Microbiology,2014,5(11):1-13.
[3]王志刚,邵平扬,曹浩强,等.创伤弧菌的培养与鉴定[J].中华医院感染学杂志,2009,19(15):2 068-2 070.
[4]Jones MK,JD Oliver.Vibrio vulnificus:disease and pathogenesis[J].Infection and Immunity,2009,77(5):1 723-1 733.
[5]李迎月,何洁仪,张维蔚,等.广州市市售水产品食源性致病菌污染状况调查[J].中国食品卫生杂志,2015,27(3):294-297.
[6]裴晓燕,余波,张秀丽,等.中国内陆6省(自治区)淡水鱼养殖、销售和餐饮环节常见嗜盐性弧菌污染调查[J].中国食品卫生杂志,2016,28(1):79-83.
[7]Olivia DN,Grieg FS.Differential specificity of selective culture media for enumeration of pathogenic vibrios:Advantages and limitations of multi-plating methods[J].Journal of Microbiological Methods,2015,111:24-30.
[8]徐景野,许国章,闫鹏,等.宁波地区海产品中副溶血性弧菌分布与研究[J].中国预防医学杂志,2012,5(13):364-369.
[9]何志凡,黎明,文君,等.2014年成都市动物性水产品及外环境中4种弧菌的污染情况分析[J].现代预防医学,2015,42(21):3 874-3 876.