2014年浙江省淡水动物性水产品中致病性弧菌污染来源分析
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摘要
目的了解淡水动物性水产品养殖、销售、加工过程中弧菌的污染来源。方法在副溶血性弧菌感染高发的第二季度和第三季度,从不同的淡水动物性水产品养殖场所、销售场所、餐饮场所采取淡水鱼、水体和水底沉积物样本;采用弧菌显色法进行致病性弧菌定量检测;并完成副溶血性弧菌分离株血清型和toxR、tdh和trh基因的检测。结果副溶血性弧菌在淡水动物性水产品中的污染与场所密切相关;副溶血性弧菌分离株的血清型以O1/O2/O3/O5为主,69株副溶血性弧菌中检出3株O3∶K6血清型,4株表达tdh基因,1株表达trh基因。结论淡水产品中致病性弧菌的污染主要来源于销售加工环节。
Objective To find the sources of pathogenetic Vibrio spp. in the farming,processing and marketing of freshwater products. Methods Freshwater products,water and sediment samples were collected from different farming,processing and marketing places in the second quarter and third quarter in 2014; to detect pathogenic Vibrio spp. by Vibrio chromogenic method and complete the detection of Vibrio parahaemolyticus isolates of serotype and toxR,tdh and trh genes. Results The contamination of pathogenetic Vibrio spp. in freshwater products is closely related to sampling sites. The serotype of Vibrio parahaemolyticus isolates is mainly O1/O2/O3/O5,and 3 O3 ∶K6 were isolated from 69 strains of Vibrio parahaemolyticus,of which 4 strains are tdh+and 1 strain is trh+. Conclusion The main pollution of pathogenic Vibrio in freshwater products is from the processing link.
Objective To find the sources of pathogenetic Vibrio spp. in the farming,processing and marketing of freshwater products. Methods Freshwater products,water and sediment samples were collected from different farming,processing and marketing places in the second quarter and third quarter in 2014; to detect pathogenic Vibrio spp. by Vibrio chromogenic method and complete the detection of Vibrio parahaemolyticus isolates of serotype and toxR,tdh and trh genes. Results The contamination of pathogenetic Vibrio spp. in freshwater products is closely related to sampling sites. The serotype of Vibrio parahaemolyticus isolates is mainly O1/O2/O3/O5,and 3 O3 ∶K6 were isolated from 69 strains of Vibrio parahaemolyticus,of which 4 strains are tdh+and 1 strain is trh+. Conclusion The main pollution of pathogenic Vibrio in freshwater products is from the processing link.
引文
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[2]Raszl SM,Froelich BA,Vieira CR,et al.Vibrio parahaemolyticus and Vibrio vulnificus in South America:water,seafood and human infections[J].J Appl Microbiol,2016,121(5):1201-1222.
[3]Elmahdi S,Da Silva LV,Parveen S.Antibiotic resistance of Vibrio parahaemolyticus and Vibrio vulnificus in various countries:A review[J].Food Microbiol,2016,57:128-134.
[4]Heng SP,Letchumanan V,Deng CY,et al.Vibrio vulnificus:An Environmental and Clinical Burden[J].Front Microbiol,2017,8:997.
[5]Turner JW,Malayil L,Guadagnoli D,et al.Detection of Vibrio parahaemolyticus,Vibrio vulnificus and Vibrio cholerae with respect to seasonal fluctuations in temperature and plankton abundance[J].Environ Microbiol,2014,16(4):1019-1028.
[6]杨娟,杨海玉,周静,等.泰州市淡水产品中副溶血性弧菌污染状况调查[J].现代预防医学,2009,36(4):639-640.
[7]高璐,杭莉,杨振泉,等.江苏省部分地区淡水产品中弧菌菌群及其致病性分析[J].中国人兽共患病学报,2013,29(2):142-147.
[8]王燕梅,唐震,乔昕,等.江苏省水产及其制品中副溶血性弧菌污染情况调查[J].中国卫生检验杂志,2016,26(19):2846-2848.
[9]吴莹,张杰,张苗,等.淄博市2014年淡水产品中致病性弧菌污染状况调查分析[J].中国卫生检验杂志,2016,26(10):1487-1489.
[10]胡元玮,吴斌,陈志清,等.水产鱼中致病性弧菌的主动监测及危险分析[J].中国卫生检验杂志,2015,25(21):3726-3727.
[11]王克波,赵金山,刘丹茹,等.2014-2016年山东省淡水产品中致病性弧菌的污染状况[J].现代预防医学,2017,44(16):2924-2927.
[12]裴晓燕,余波,张秀丽,等.中国内陆6省(自治区)淡水鱼养殖、销售和餐饮环节常见嗜盐性弧菌污染调查[J].中国食品卫生杂志,2016,28(1):79-83.
[13]Wendling CC,Batista FM,Wegner KM.Persistence,seasonal dynamics and pathogenic potential of Vibrio communities from Pacific oyster hemolymph[J].PLo S ONE,2014,9(4):e94256.
[14]Di DY,Lee A,Jang J,et al.Season-specific occurrence of potentially pathogenic Vibrio spp.on the Southern Coast of South Korea[J].Appl Environ Microbiol,2016,83(3):e02680-16.
[15]Sullivan TJ,Neigel JE.Effects of temperature and salinity on prevalence and intensity of infection of blue crabs,Callinectes sapidus,by Vibrio cholerae,V.parahaemolyticus,and V.vulnificus in Louisiana[J].J Invertebr Pathol,2017,17(1):151.