Cu_2O微球修饰电极对动物源食品中土霉素的电化学测定
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摘要
利用Cu_2O微球修饰玻碳电极研究了土霉素的电化学行为,发现土霉素的氧化是一个受吸附控制的一质子、一电子参与的不可逆过程.在pH 4.8HAc-NaAc缓冲溶液中,开路富集180s后利用方波伏安法发展了高灵敏检测土霉素的电化学方法,线性范围为0.5～210"mol/L,检出限为0.15"mol/L(R_(SN)=3).该方法用于测定动物源食品中土霉素,回收率介于97.4%～107%之间.
The electrochemical behaviors of oxytetracycline on Cu_2 O microspheres modified glassy carbon electrode were studied,and it was found that the oxidation of oxytetracycline was an adsorption-controlled,one-proton,one-electron,and irreversible process.In pH 4.8 HAc-NaAc buffer solution,an electrochemical method with high sensitivity was developed for the determination of oxytetracycline by using square wave voltammetry after an open-circuit accumulation at 0.4 V.The linear range was 0.5～210 "mol/L and the limit of detection was 0.15 " mol/L(R_(SN)= 3).Finally,this method was applied for the determination of oxytetracycline in animal-derived food,and the recoveries were between 97.4%～107%.
The electrochemical behaviors of oxytetracycline on Cu_2 O microspheres modified glassy carbon electrode were studied,and it was found that the oxidation of oxytetracycline was an adsorption-controlled,one-proton,one-electron,and irreversible process.In pH 4.8 HAc-NaAc buffer solution,an electrochemical method with high sensitivity was developed for the determination of oxytetracycline by using square wave voltammetry after an open-circuit accumulation at 0.4 V.The linear range was 0.5～210 "mol/L and the limit of detection was 0.15 " mol/L(R_(SN)= 3).Finally,this method was applied for the determination of oxytetracycline in animal-derived food,and the recoveries were between 97.4%～107%.
引文
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[3]GAN Tian,WANG Zhikai,SHI Zhaoxia,et al.Graphene oxide reinforced core shell structured Ag@Cu2O with tunable hierarchical morphologies and their morphology dependent electrocatalytic properties for bio-sensing applications[J].Biosensors and Bioelectronics,2018,112:23-30.
[4]WANG Jianlong,ZHUAN Run,CHU Libing,et al.The occurrence,distribution and degradation of antibiotics by ionizing radiation:An overview[J].Science of the Total Environment,2019,646:1385-1397.
[5]RASMUSSEN S H.,SHRESTHA S,BJERREGAARD L G,et al.Antibiotic exposure in early life and childhood overweight and obesity:A systematic review and meta-analysis[J].Diabetes Obesity and Metabolism,2018,20(6):1508-1514.
[6]王海波,董高丽,白宏宇,等.基于BSA模板荧光铜纳米簇的生物传感方法灵敏检测四环素[J].信阳师范学院学报(自然科学版),2018,31(4):632-635.WANG Haibo,DONG Gaoli,BAI Hongyu,et al.Fluorescence sensing strategy for sensitive detection of tetracycline based on bovine serum albumin templated copper nanoclusters[J].Journal of Xinyang Normal University(Natural Science Edition),2018,31(4):632-635.
[7]GAN Tian,SHI Zhaoxia,SUN Junyong,et al.Simple and novel electrochemical sensor for the determination of tetracycline based on iron/zinc cations-exchanged montmorillonite catalyst[J].Talanta,2014,121:187-193.
[8]SUN Junyong,GAN Tian,ZHU Haijing,et al.Direct electrochemical sensing for oxytetracycline in food using a zinc cation-exchanged montmorillonite[J].Applied Clay Science,2014,101:598-603.
[9]KERRIGAN J F.,SANDBERG K D.,ENGSTROM D R,el al.Sedimentary record of antibiotic accumulation in Minnesota Lakes[J].Science of the Total Environment,2018,621:970-979.
[10]CORNEJO J,POKRANT E,KROGH M,et al.Determination of oxytetracycline and 4-epi-oxytetracycline residues in feathers and edible tissues of broiler chickens using liquid chromatography coupled with tandem mass spectrometry[J].Journal of Food Protection,2017,80(4):619-625.
[11]RODRIGUEZ-DORADO R,CARRO A M,CHIANELLA L,et al.Oxytetracycline recovery from aqueous media using computationally designed molecularly imprinted polymers[J].Analytical and Bioanalytical Chemistry,2016,408(24):6845-6856.
[12]ZHANG Li,WANG Hui.Cuprous oxide nanoshells with geometrically tunable optical properties[J].ACS Nano,2011,5(4):3257-3267.
[13]曹俊涛,任秀燕,王玉玲,等.基于鲁米诺功能化纳米金的电化学发光适配体传感器灵敏检测癌胚抗原[J].信阳师范学院学报(自然科学版),2018,31(2):254-257.CAO Juntao,REN Xiuyan,WANG Yuling,et al.Electrochemiluminescence aptasensor for sensitive detection of carcinoembryonic antigen based on luminol-functionalized gold nanoparticles[J].Journal of Xinyang Normal University(Natural Science Edition),2018,31(2):254-257.
[14]KUREK M.Pseudoallergic reactions.Intolerance to natural and synthetic food constituents masquerading as food allergy[J].Pediatria Polska,1996,71(9):743-752.
[15]XIE Shaowen,XU Jianxiong,DU Jingjing,et al.Preparation of Ni-Co alloy electrodes by pulsed electrodeposition and its application in detection of oxytetracycline[J].Nanoscience and Nanotechnology Letters,2016,8(6):527-531.
[16]KRUID J,FOGEL R,LIMSON J.Voltammetric investigation of complex growth media at a bare glassy carbon electrode:A case study of oxytetracycline[J].Electrochimica Acta,2014,128:41-47.