苏州地区动物源食品中氯霉素残留检测及风险评估
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摘要
目的采用固相萃取-高效液相色谱-串联三重四级质谱联用法(SPE-UPLC-MS/MS)测定苏州地区动物源食品中氯霉素残留。方法氯霉素经乙酸乙酯提取,C_(18)固相萃取柱净化,采用电喷雾负离子扫描,高效液相色谱-串联质谱在多反应监测模式下检测,内标法定量。结果氯霉素在0.05 ng/ml～1.20 ng/ml线性关系良好,相关系数为0.999 1,方法加标回收率为93.0%～102.0%,检出限为4.9×10~(-4)μg/kg～5.6×10~(-4 )μg/kg。所有鸡蛋、鸡肉、猪肉样品中均未检出氯霉素,2份双壳类检出,检出率为2.1%,对氯霉素残留的人体健康风险评估表明IFSc远<1。结论苏州地区水产品中氯霉素残留对人体健康不存在明显危害。
Objective Chloramphenicol residue was detected in animal-derived food in Suzhou by solid phase extraction-high performance liquid chromatography-tandem spectrometry(SPE-HPLC-MS/MS). Methods Chloramphenicol residue was extracted with ethylacetate and purified with C_(18) SPE catridge, then determined by UPLC-MS/MS with negative electrospray ionization(ESI~-) in multiple reaction monitoring(MRM) mode, and quantified with internal standard method. Results The correlation coefficient was 0.999 1 when the concentration of chloramphenicol was within 0.05 ng/ml-1.20 ng/ml. The recoveries of the method were within 93.0%-102.0%, with the detection limits of 4.9×10~(-4 )μg/kg-5.6×10~(-4 )μg/kg. Chloramphenicol was not detected in eggs, chicken and pork samples, except two bivalve samples(2.1%). The risk assessment showed that the IFSc<<1. Conclusion The results indicate that the chloramphenicol risk is low to human health.
Objective Chloramphenicol residue was detected in animal-derived food in Suzhou by solid phase extraction-high performance liquid chromatography-tandem spectrometry(SPE-HPLC-MS/MS). Methods Chloramphenicol residue was extracted with ethylacetate and purified with C_(18) SPE catridge, then determined by UPLC-MS/MS with negative electrospray ionization(ESI~-) in multiple reaction monitoring(MRM) mode, and quantified with internal standard method. Results The correlation coefficient was 0.999 1 when the concentration of chloramphenicol was within 0.05 ng/ml-1.20 ng/ml. The recoveries of the method were within 93.0%-102.0%, with the detection limits of 4.9×10~(-4 )μg/kg-5.6×10~(-4 )μg/kg. Chloramphenicol was not detected in eggs, chicken and pork samples, except two bivalve samples(2.1%). The risk assessment showed that the IFSc<<1. Conclusion The results indicate that the chloramphenicol risk is low to human health.
引文
[1] 高林.氯霉素的不良反应及其残留检测方法研究进展[J].动物医学进展,2010,31(4):90-95.
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[4] 高林. 氯霉素的不良反应及其残留检测方法研究进展[J]. 动物医学进展, 2010, 31(4): 90-95.
[5] 高何刚. 氯霉素残留的液相色谱质谱检测方法的样品前处理研究进展[J]. 广东化工, 2017,44(8):204-205.
[6] 薛金英. 酶联免疫吸附法(ELISA)检测鸡肉中氯霉素[J]. 中国动物保健, 2016,18(10):79-80.
[7] 华娟,方勤美,熊春娥,等.市售动物源性食品中氯霉素类药物残留量的调查研究[J]. 食品安全质量检测学报, 2013, 4(1): 165-170.
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[9] 金征宇,胥传来,谢正军.食品安全导论[M].北京:化学工业出版社,2005:75-82,255-257.
[10] USEPA. The Ecotoxicology Database (ECOTOX)[DB/OL][2015-11-10]. http://cfpub.epa.gov/ecotox/ecotox_home.cfm.
[11] 陈俊玉,何建顺,于晓薇,等.出口水产品中氯霉素残留状况及其风险评价[J]. 食品研究与开发, 2016, 37(13): 181-183.
[12] 谢秋菊,许静,王秀娟.水产品中氯霉素的残留状况[J]. 食品研究与开发, 2012, 33(1): 138-140.
[13] 王明珠,陈玲,赵宏宇,等.养殖对虾中抗生素的残留及其健康风险[J]. 环境监测管理与技术, 2018, 30(2): 34-38.
[14] 张文礼.广州15批化妆品检出甲硝唑等禁用物质[J]. 广西质量监督导报, 2014(11): 11-11.
[15] 保障水产品质量安全我们在监管什么[J]. 北京农业: 上旬刊, 2015(7): 14-16.
[2] Masahiko T, Shigeki D, Taketoshi N. Determination of chloramphenicol residues in fish meats by liquid chromatography-atmospheric pressure photoionization mass spectrometry[J]. J Chromatogr A, 2003, 1011(1/2): 67-75.
[3] 蒋定国, 杨大进. 动物性食品中氯霉素残留检测技术的研究概况[J]. 中国食品卫生杂志, 2002, 14(2): 44-47.
[4] 高林. 氯霉素的不良反应及其残留检测方法研究进展[J]. 动物医学进展, 2010, 31(4): 90-95.
[5] 高何刚. 氯霉素残留的液相色谱质谱检测方法的样品前处理研究进展[J]. 广东化工, 2017,44(8):204-205.
[6] 薛金英. 酶联免疫吸附法(ELISA)检测鸡肉中氯霉素[J]. 中国动物保健, 2016,18(10):79-80.
[7] 华娟,方勤美,熊春娥,等.市售动物源性食品中氯霉素类药物残留量的调查研究[J]. 食品安全质量检测学报, 2013, 4(1): 165-170.
[8] 蒋长征,张立军,戎江瑞,等.宁波市鲜活水产品抗生素残留调查研究[J]. 中国卫生检验杂志, 2007, 17(5): 902-904.
[9] 金征宇,胥传来,谢正军.食品安全导论[M].北京:化学工业出版社,2005:75-82,255-257.
[10] USEPA. The Ecotoxicology Database (ECOTOX)[DB/OL][2015-11-10]. http://cfpub.epa.gov/ecotox/ecotox_home.cfm.
[11] 陈俊玉,何建顺,于晓薇,等.出口水产品中氯霉素残留状况及其风险评价[J]. 食品研究与开发, 2016, 37(13): 181-183.
[12] 谢秋菊,许静,王秀娟.水产品中氯霉素的残留状况[J]. 食品研究与开发, 2012, 33(1): 138-140.
[13] 王明珠,陈玲,赵宏宇,等.养殖对虾中抗生素的残留及其健康风险[J]. 环境监测管理与技术, 2018, 30(2): 34-38.
[14] 张文礼.广州15批化妆品检出甲硝唑等禁用物质[J]. 广西质量监督导报, 2014(11): 11-11.
[15] 保障水产品质量安全我们在监管什么[J]. 北京农业: 上旬刊, 2015(7): 14-16.