电针结合天麻素对局灶性脑缺血大鼠海马CA3区GAP-43和SYN表达的影响
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摘要
目的:验证电针结合天麻素对局灶性脑缺血大鼠海马CA3区生长相关蛋白-43(Growth-associated protein,GAP-43)和突触素(Synaptophysin,SYN)表达是否有协同增效作用。方法:SD大鼠40只随机分为假手术组、模型组、电针组、天麻素组和电针结合天麻素组。采用线栓法阻塞大鼠右侧大脑中动脉复制局灶性脑缺血模型。造模成功后,按分组分别及同时给予电针刺激左侧曲池与合谷穴30min,天麻素注射液10mg/kg腹腔注射,1次/d,连续治疗14d。免疫组织化学SABC法检测海马GAP-43和SYN的表达。结果:与假手术组比较,模型组大鼠缺血侧海马CA3区GAP-43阳性神经元数量增多(P<0.05),SYN阳性神经元数量减少(P<0.05);与模型组比较,电针组、天麻素组和电针结合天麻素组大鼠缺血侧海马CA3区GAP-43和SYN阳性神经元数量均显著增多(P<0.05);电针结合天麻素组GAP-43和SYN阳性神经元数量均较电针组、天麻素组增多(均P<0.05)。结论:电针结合天麻素对缺血侧海马CA3区GAP-43和SYN的表达具有协同增效作用,可更好地促进缺血损伤神经元的再生修复。
Objective:To validate whether there is synergy effect of electroacupuncture(EA)combined with gastrodin on the expression of(Growth-associated protein,GAP-43)and(Synaptophysin,SYN)in hippocampal CA3 in focal cerebral ischemia(FCI)rats.Methods:Forty SD rats were randomly divided into sham operation,model,EA,gastrodin and EA+ gastrodin groups.FCI rats were induced by middle cerebral artery occlusion with thread embolus.EA was applied to left side"Quchi"(LI 11)and"Hegu"(LI 4)after MCAO,gastrodin at 10mg/kg was given to rats by intraperitoneal.The expressions of GAP-43 and SYN in hippocampal CA3 detected by immunohistochemical.Results:Compared with sham operation group,the number of GAP-43 positive neurons increased(P<0.05),the number of SYN positive neurons decreased(P<0.05).Compared with model group,the number of GAP-43 and SYN positive neurons increased(P<0.05),expression levels in the EA+gastrodin group increased significantly than the EA or gastrodin group(P<0.05).Conclusion:EA combined with gastrodin have synergistic effect on the expression of GAP-43 and SYN in the ischemic hippocampus CA3,can promote the regeneration and repair of injured neurons.
Objective:To validate whether there is synergy effect of electroacupuncture(EA)combined with gastrodin on the expression of(Growth-associated protein,GAP-43)and(Synaptophysin,SYN)in hippocampal CA3 in focal cerebral ischemia(FCI)rats.Methods:Forty SD rats were randomly divided into sham operation,model,EA,gastrodin and EA+ gastrodin groups.FCI rats were induced by middle cerebral artery occlusion with thread embolus.EA was applied to left side"Quchi"(LI 11)and"Hegu"(LI 4)after MCAO,gastrodin at 10mg/kg was given to rats by intraperitoneal.The expressions of GAP-43 and SYN in hippocampal CA3 detected by immunohistochemical.Results:Compared with sham operation group,the number of GAP-43 positive neurons increased(P<0.05),the number of SYN positive neurons decreased(P<0.05).Compared with model group,the number of GAP-43 and SYN positive neurons increased(P<0.05),expression levels in the EA+gastrodin group increased significantly than the EA or gastrodin group(P<0.05).Conclusion:EA combined with gastrodin have synergistic effect on the expression of GAP-43 and SYN in the ischemic hippocampus CA3,can promote the regeneration and repair of injured neurons.
引文
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[3]蔡玉颖,刘志顺,王顺,等.电针曲池、足三里穴对脑缺血再灌注损伤大鼠神经功能缺损的影响〔J〕.上海中医药大学学报,2009,23(6):64-66.
[4]王可博,庞国军,方智松.针刺合谷透后溪治疗中风偏瘫后手指拘挛127例〔J〕.上海针灸杂志,2008,27(1):35.
[5]吴锋,李怀斌,赵健,等.电针结合天麻素对局灶性脑缺血大鼠神经功能、额叶皮质勿动蛋白A及其受体表达的影响〔J〕.针刺研究,2016,41(1):65-69.
[6]李怀斌,吴锋,熊克仁,等.针药结合对局灶性脑缺血大鼠海马CA1和CA3区神经干细胞增殖与分化相关蛋白表达的影响〔J〕.针刺研究,2015,40(6):455-460.
[7]Nguyen L,He Q,Meiri KF.Regulation of GAP-43at serine41acts as a switch to modulate both intrinsic and extrinsic behaviors of growing neurons,via altered membrane distribution〔J〕.Mol Cell Neurosci,2009,41(1):62-73.
[8]段小东,张雷,胥方元,等.行为学训练对局灶性脑缺血大鼠梗死侧海马CA3区突触结构的影响〔J〕.中华康复医学杂志,2012,27(5):1001-1242.
[9]毕天,李彦文,段靖远,等.天麻抗老年痴呆有效成分的研究进展〔J〕.中医药导报,2013,19(2):103-105.
[10]杨杰,赵朝华,宗长宏,等.短暂性MCAO大鼠注射天麻素具有神经保护作用〔J〕.第四军医大学学报,2008,29(4):295-296.
[11]王德伟,刘黎青,周盛.针药结合对实验性脑缺血作用机制的研究进展〔J〕.山东中医杂志,2009,28(12):881-882.
[12]魏彦珍,刘轲,张怀亮.针药结合治疗脑梗死机制研究进展〔J〕.辽宁中医药大学学报,2014,16(2):217-219.
[13]崔晶晶,高俊虹,王玉敏,等.针药结合增效机制研究的新思路探讨〔J〕.针刺研究,2010,35(2):146-149.