土荆芥精油对灰茶尺蠖幼虫的熏蒸活性及对其体内两种酶活力的影响
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摘要
采用皿内测定法,研究了土荆芥精油对灰茶尺蠖的熏蒸活性及对其体内2种解毒酶活性的影响。结果表明,随着土荆芥精油剂量的增加和处理时间的延长,灰茶尺蠖幼虫的死亡率也相应升高;LC_(50)随着处理时间的延长,逐渐降低;通过测定土荆芥精油对灰茶尺蠖幼虫体内解毒酶活力变化表明,其精油对灰茶尺蠖幼虫体内的乙酰胆碱酯酶呈明显的抑制作用,对羧酸酯酶呈激活后抑制的作用。
Fumigant activity of Chenopodium ambrosioi des essential oil and it's effect on two enzymes of Ectropis grisescens were studied. Corrected mortality of E. grisescens larvae showed dose-and time-dependent increases with C.ambrosioides essential oil treatment. The LC_(50) decreased gradually with increment of treatment time. The oil showed inhibition effect on acetylcholinesterase, and showed inhibition effect after activation on carboxylesterase in E. grisescens.
Fumigant activity of Chenopodium ambrosioi des essential oil and it's effect on two enzymes of Ectropis grisescens were studied. Corrected mortality of E. grisescens larvae showed dose-and time-dependent increases with C.ambrosioides essential oil treatment. The LC_(50) decreased gradually with increment of treatment time. The oil showed inhibition effect on acetylcholinesterase, and showed inhibition effect after activation on carboxylesterase in E. grisescens.
引文
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[3]Guo S S,You C Y,Liang J Y,et al.Chemical composition and bioactivities of the essential oil from Etlingera yunnanensis against two stored product insects[J].Molecules,2015,20(9):15735-15747.
[4]马玉华,赵忠,江志利,等.苦杏仁精油对粘虫的触杀活性研究[J].西北植物学报,2010,30(3):604-609.
[5]Yang K,You C X,Wang C F,et al.Chemical composition and bioactivity of essential oil of Atalantia guillauminii against three species stored product insects[J].Journal of Oleo Science,2015,64(10):1101-1109.
[6]Li S G,Li M Y,Huang Y Z,et al.Fumigant activity of Illicium verum fruit extracts and their effects on the acetylcholinesterase and glutathione S-transferase activities in adult Sitophilus zeamais[J].Journal of Pest Science,2013,86(4):677-683.
[7]Chun S S,Hu J F,Liu Z L.Composition of essential oil of chinese Chenopodium ambrosioides and insecticidal activity against maize weevil,Sitophilus zeamains[J].Pest Management Science,2011,67(6):714-718.
[8]陈利军,夏新奎,刘红敏,等.新鲜土荆芥挥发油的抑菌活性及成分分析[J].江苏农业科学,2010(5):452-454.
[9]陈利军,智亚楠,王国君,等.土荆芥花序和叶挥发油的抑菌作用及组分分析[J].植物保护,2014,40(5):40-43.
[10]Chiasson H,Vincent C,Bostanian N J.Insectticidal properties of a chenopodium-based botanical[J].Journal of Economic Entomology,2004,97(4):1373-1377.
[11]Cloyd R A,Chiasson H.Activity of an essential oil derived from Chenopodium ambrosioides on greenhouse insect pests[J].Horticultural Entomology,2007,100(2):459-466.
[12]赵建伟,魏辉,赵士熙,等.土荆芥生物总碱对家蝇的毒杀作用及药剂敏感性的影响[J].华东昆虫学报,2007,16(1):64-69.
[13]黄九玲,程怡慧,徐艳霞,等.土荆芥提取物对谷蠹和赤拟谷盗的触杀与熏蒸活性研究[J].中国粮油学报,2013,28(7):66-69.
[14]孙红霞,魏辉,赵建伟,等.土荆芥精油预处理后小菜蛾幼虫对氟虫腈敏感度的变化[J].华东昆虫学报,2008,17(1):9-13.
[15]黄玉清,魏辉,刘剑,等.土荆芥精油对菜青虫毒杀、拒食和生长抑制作用的研[J].福建农业学报,2007,22(3):266-270.
[16]刘玉坤,王渭霞,傅强,等.寄主植物对3种稻飞虱解毒酶和保护酶活性的影响[J].中国水稻科学,2011,25(6):659-666.