紫檀芪对2型糖尿病大鼠肾脏的影响
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摘要
目的:研究紫檀芪对2型糖尿病大鼠肾脏的影响。方法:采用高糖高脂饮食结合小剂量链脲菌素构建2型糖尿病大鼠模型。将大鼠随机分为正常对照组、2型糖尿病组、紫檀芪低剂量组、紫檀芪中剂量组、紫檀芪高剂量组。干预7w后,检测大鼠血糖和血脂的变化,测定肾功能指标血尿素氮和血肌酐的含量及肾脏氧化应激水平,取肾脏组织做HE染色,观察大鼠肾组织病理学变化。结果:2型糖尿病组大鼠血糖、甘油三酯(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇(LDL-C)水平升高,低密度脂蛋白胆固醇(HDL-C)水平降低;血尿素氮和血肌酐升高;肾脏组织的超氧化物歧化酶(SOD)活性下降,丙二醛(MDA)水平升高;肾组织局部可见大量炎性细胞灶性浸润,炎症灶周围肾小管上皮细胞广泛水肿。紫檀芪干预后,血糖、TG、TC、LDL-C水平降低;血尿素氮和血肌酐降低;肾脏组织的SOD活性升高,MDA水平下降;肾小球和肾小管病变减轻。结论:紫檀芪能够降低血糖和血脂、血尿素氮和血肌酐,改善肾脏的病理损伤,对2型糖尿病大鼠肾脏的损伤具有一定的治疗作用。
Objective To explore the influence of pterostilbene on renal tissues of type 2 diabetic rats. Method Type 2 diabetes model( T2 DM) was induced by high-fat and high-sugar diet combined with streptozotocin( 40 mg/kg). Rats were randomly divided into normal control group,type 2 diabetes mellitus( T2 DM) group,PTE low dose group,PTE middle dose group and PTE high dose group. After treatment of pterostilbene,fasting blood glucose( FBG) and blood lipid,BUN and SCr were examined,the activity of SOD and the level of MDA in renal tissues were evaluated,the morphology of kidney was observed. Result It was illustrated that FBG,TG,TC,LDL-C,BUN and SCr were increased,the activity of SOD reduced,and the level of MDA augmented in T2 DM group. A large number of inflammatory cell infiltrated the renal tissues,and the extensive edema of renal tubular epithelial cells were found. After intervention,FBG,TG,TC,LDL-C,BUN and SCr were decreased,the activity of SOD increased,and the level of MDA reduced. The morphological change of kidney was relived. Conclusion Pterostilbene can reduce FBG and blood lipid,BUN and SCr,improve the pathological damage of kidney,therefore,have certain therapeutic effect on kidney in T2 DM rats.
Objective To explore the influence of pterostilbene on renal tissues of type 2 diabetic rats. Method Type 2 diabetes model( T2 DM) was induced by high-fat and high-sugar diet combined with streptozotocin( 40 mg/kg). Rats were randomly divided into normal control group,type 2 diabetes mellitus( T2 DM) group,PTE low dose group,PTE middle dose group and PTE high dose group. After treatment of pterostilbene,fasting blood glucose( FBG) and blood lipid,BUN and SCr were examined,the activity of SOD and the level of MDA in renal tissues were evaluated,the morphology of kidney was observed. Result It was illustrated that FBG,TG,TC,LDL-C,BUN and SCr were increased,the activity of SOD reduced,and the level of MDA augmented in T2 DM group. A large number of inflammatory cell infiltrated the renal tissues,and the extensive edema of renal tubular epithelial cells were found. After intervention,FBG,TG,TC,LDL-C,BUN and SCr were decreased,the activity of SOD increased,and the level of MDA reduced. The morphological change of kidney was relived. Conclusion Pterostilbene can reduce FBG and blood lipid,BUN and SCr,improve the pathological damage of kidney,therefore,have certain therapeutic effect on kidney in T2 DM rats.
引文
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[11]孙培荣,石永兵.尿白蛋白/肌酐比值对诊断早期糖尿病肾病的价值[J].现代临床医学,2007,33(1):3-4.
[12]Pitocco D,Tesauro M,Alessandro R,et al.Oxidative stress in diabetes:implication for vascular and other complications[J].Int J Mol Sci,2013,14(11):21525-21550.
[13]Rubattu S,Mennuni S,Testa M,et al.Pathogenesis of chronic cardiorenal syndrome:is there a role for oxidative stress?[J].Int J Mol Sci,2013,14(11):23011-23032.
[2]中华医学会糖尿病学分会.中国2型糖尿病防治指南[J].中国医学前言杂志,2015,7(3):26-89.
[3]Fan LL,Tu PF,Chen HB,et al.Simultaneous quantification of five major constituents in stems of Dracaena plants and related medicinal preparations from China and Vietnam by HPLC-DAD[J].Biomed Chromatogr,2009,23(11):1191-1200.
[4]Mc Cormack D,Mc Fadden D.A review of pterostilbene antioxidant activity and disease modification[J].Oxid Med Cell Longev,2013(2013):1-15.
[5]Pari L,Satheesh MA.Effect of pterostilbene on hepatic key enzymes of glucose metabolism in streptozotocin-and nicotinamide-induced diabetic rats[J].Life Sci,2006,79(7):641-645.
[6]Rimando AM,Nagmani R,Feller DR,et al.Pterostilbene,a new agonist for the peroxisome proliferator-activated receptor alpha-isoform,lowers plasma lipoproteins and cholesterol in hypercholesterolemic hamsters[J].J Agric Food Chem,2005,53(9):3403-3407.
[7]刘丹丹,张伟,朱浩,等.Ⅱ型糖尿病大鼠动物模型的构建与验证[J].中国兽医学报,2016,36(7):1201-1205.
[8]刘欣欣,孙华磊,王腾,等.紫檀芪对2型糖尿病大鼠血脂的改善及对PPARγ的调控作用[J].现代食品科技,2018,34(9):1-6.
[9]李冰,王静凤,杨延存,等.蛹虫草对糖尿病大鼠肾脏病程发展的抑制作用[J].食品科学,2011,32(15):255-259.
[10]刘月娇,徐雷雷,柳东,等.海参磷脂型二十碳五烯酸对链脲佐菌素诱导的糖尿病大鼠肾脏功能改善作用的研究[J].食品工业科技,2012,33(19):331-334.
[11]孙培荣,石永兵.尿白蛋白/肌酐比值对诊断早期糖尿病肾病的价值[J].现代临床医学,2007,33(1):3-4.
[12]Pitocco D,Tesauro M,Alessandro R,et al.Oxidative stress in diabetes:implication for vascular and other complications[J].Int J Mol Sci,2013,14(11):21525-21550.
[13]Rubattu S,Mennuni S,Testa M,et al.Pathogenesis of chronic cardiorenal syndrome:is there a role for oxidative stress?[J].Int J Mol Sci,2013,14(11):23011-23032.