标本放置时间对全自动血沉分析仪测定结果的影响
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摘要
目的探讨血沉标本在常温下的放置时间对全自动血沉分析仪测定结果的影响。方法采集2018年10月自贡市第四人民医院48例门诊患者的静脉血标本,每例患者抽取2管,一管采血后于室温放置0、4、6、8、10、12 h,采用Roller 20全自动血沉分析仪检测红细胞沉降率(ESR),另一管于采血后即刻采用传统魏氏法检测ESR。将传统魏氏法的检测结果作为参考标准,与全自动血沉仪采血后即刻测得的ESR进行比较;比较全自动血沉仪采血后0、4、6、8、10、12 h测得的ESR结果。记录所有数据并进行统计学分析。结果全自动血沉仪采血后即刻测得的ESR与传统魏氏法比较差异无统计学意义(mm/1 h:11.06±6.63比10.73±6.96,P>0.05)。与采血后即刻比较,随着采血时间的延长,全自动血沉仪测得的ESR值逐渐下降,至采血后10、12 h测得的ESR已与0 h时比较出现了统计学差异(mm/1 h:7.68±5.89,5.08±4.89比11.06±6.63,均P<0.05),采血后4、6、8 h测得的ESR则与0 h时比较差异均无统计学意义(均P>0.05)。结论如果采血后立即进行检测,全自动血沉仪测得的血沉结果与传统魏氏法结果相似。由于全自动血沉仪检测快速、准确,可以广泛应用于实验室,但要注意在室温情况下全自动血沉仪检测血沉应在8 h内完成,以免检测结果不准确。
Objective To explore the influence of the time of erythrocyte sedimentation rate(ESR specimen placed at room temperature on the results of automatic ESR analyzer. Methods The venous blood samples were collected from 48 outpatients in the Fourth People's Hospital of Zigong City in October 2018. Two tubes of venous blood were taken from each patient, one of them was placed at room temperature for 0, 4, 6, 8,10 and 12 hours, and Roller 20 full automatic ESR analyzer was used to detect ESR; the traditional Wechsler assay was used to detect the ESR for the blood in another tube immediately after its collection. The ESR result of traditional Wechsler method was taken as a reference standard and compared with the ESR measured immediately after blood collection by the automatic ESR analyzer; the ESR levels measured at 0, 4, 6, 8, 10 and 12 h after blood collection by automatic ESR analyzer were compared among various time points. All data were recorded and statistically analyzed. Results There was no statistically significant difference between ESR measured by automatic ESR analyzer and traditional Widmanstatten method(mm/1 h: 11.06±6.63 vs. 10.73±6.96, P > 0.05).With the prolongation of blood collection time, ESRs measured by automatic ESR instrument gradually decreased,ESR at 10 hours and 12 hours after blood collection were decreased significantly as compared with that immediately after blood collection(mm/1 h: 7.68±5.89, 5.08±4.89 vs. 11.06±6.63, all P < 0.05), and the ESRs measured at 4, 6 and 8 hours after blood collection did not change significantly(all P > 0.05). Conclusions If blood is tested immediately after collected, there is no statistical significant difference between the results of automatic ESR instrument and those of Wei's method. Because of the analyzer's speed and accuracy, it can be widely used in the laboratory. However, it should be noted that the automatic ESR test should be completed within 8 hours at room temperature; otherwise, the test result can be inaccurate.
Objective To explore the influence of the time of erythrocyte sedimentation rate(ESR specimen placed at room temperature on the results of automatic ESR analyzer. Methods The venous blood samples were collected from 48 outpatients in the Fourth People's Hospital of Zigong City in October 2018. Two tubes of venous blood were taken from each patient, one of them was placed at room temperature for 0, 4, 6, 8,10 and 12 hours, and Roller 20 full automatic ESR analyzer was used to detect ESR; the traditional Wechsler assay was used to detect the ESR for the blood in another tube immediately after its collection. The ESR result of traditional Wechsler method was taken as a reference standard and compared with the ESR measured immediately after blood collection by the automatic ESR analyzer; the ESR levels measured at 0, 4, 6, 8, 10 and 12 h after blood collection by automatic ESR analyzer were compared among various time points. All data were recorded and statistically analyzed. Results There was no statistically significant difference between ESR measured by automatic ESR analyzer and traditional Widmanstatten method(mm/1 h: 11.06±6.63 vs. 10.73±6.96, P > 0.05).With the prolongation of blood collection time, ESRs measured by automatic ESR instrument gradually decreased,ESR at 10 hours and 12 hours after blood collection were decreased significantly as compared with that immediately after blood collection(mm/1 h: 7.68±5.89, 5.08±4.89 vs. 11.06±6.63, all P < 0.05), and the ESRs measured at 4, 6 and 8 hours after blood collection did not change significantly(all P > 0.05). Conclusions If blood is tested immediately after collected, there is no statistical significant difference between the results of automatic ESR instrument and those of Wei's method. Because of the analyzer's speed and accuracy, it can be widely used in the laboratory. However, it should be noted that the automatic ESR test should be completed within 8 hours at room temperature; otherwise, the test result can be inaccurate.
引文
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2刘成玉,罗春丽.临床检验基础[M].5版.北京:人民卫生出版社,2012:39.
3邹东华,黄瑞亮,黄成彦.血沉测定结果在疾病鉴别中的作用[J].中国误诊学杂志,2002,2(2):244-245.DOI:10.3969/j.issn.1009-6647.2002.02.060.
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5韩呈武,刘海霞.全自动化血沉仪参考范围建立及临床应用评价[J].现代中西医结合杂志,2008,17(13):2032-2032.DOI:10.3969/j.issn.1008-8849.2008.13.077.