牛初乳冻干粉及其产品中EGF·TGF-α和IGF-1的含量测定及其变化
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摘要
[目的]研究牛初乳冻干粉(LBC)及其产品中表皮生长因子(EGF)、转化生长因子-α(TGF-α)和类胰岛素生长因子-1(IGF-1)含量的变化。[方法]采用冷冻干燥工艺制备0～7 d的LBC和常乳冻干粉(LM),采用酶联免疫分析法测定EGF、TGF-α和IGF-1的含量。[结果]2 d内牛初乳冻干后平均干物质含量为200 g/L。第1天LBC中EGF(7.0 ng/g)、TGF-α(16.8 ng/g)和IGF-1(3.36μg/g)的含量最高,此后快速下降,第7天接近LM水平。第0～4天LBC中EGF、TGF-α和IGF-1的平均含量分别为5.6 ng/g、9.6 ng/g和2.42μg/g,分别为LM的7.0倍、13.7倍和6.1倍。3种牛初乳保健食品的EGF和TGF-α含量显著高于LM和2种市售奶粉。初乳素的EGF和TGF-α含量高于其他2种牛初乳保健食品。初乳素的IGF-1含量为1.11μg/g,极显著高于LM。[结论]研究可为牛初乳产品的进一步开发利用提供理论和试验依据。
[Objective] To study the changes of contents of epidermal growth factor(EGF), transforming growth factor-α(TGF-α) and insulin-like growth factor-1(IGF-1) in lyophilized bovine colostrums(LBC) during the lactation of 7 days and their contents in bovine colostrums products. [Method] Bovine colostrums from the first day to the 7~(th) days postpartum and milk were prepared into LBC and LM by the freeze-drying technology, and the contents of EGF, TGF-α and IGF-1 were analyzed by enzyme-linked immunoassay. [Result] The average dry matter content of bovine colostrums within 2 days was 200 g/L by lyophilization. The highest content of EGF(7.0 ng/g), TGF-α(16.8 ng/g) and IGF-1(3.36 μg/g) was found in the LBC of the first day postpartum, then rapidly decreased within 7 days, and the contents on the 7~(th) day were close to LM levels. The average content of EGF, TGF-α and IGF-1 in LBC within 4 days were 5.6 ng/g, 9.6 ng/g, 2.42 μg/g, which were 7.0, 13.7 and 6.1 times as that in LM. The contents of EGF, TGF-α in three bovine colostrums health-care food(BCHF) were significantly higher than two market milk powder and LM. The contents of EGF, TGF-α in prolactin were significantly higher than other two bovine colostrum health food. The content of IGF-1 in prolactin was 1.11 μg/g, which was significantly higher than that in LM.[Conclusion] The research could provide theoretical and experimental basis for the further development and utilization of bovine colostrum products.
[Objective] To study the changes of contents of epidermal growth factor(EGF), transforming growth factor-α(TGF-α) and insulin-like growth factor-1(IGF-1) in lyophilized bovine colostrums(LBC) during the lactation of 7 days and their contents in bovine colostrums products. [Method] Bovine colostrums from the first day to the 7~(th) days postpartum and milk were prepared into LBC and LM by the freeze-drying technology, and the contents of EGF, TGF-α and IGF-1 were analyzed by enzyme-linked immunoassay. [Result] The average dry matter content of bovine colostrums within 2 days was 200 g/L by lyophilization. The highest content of EGF(7.0 ng/g), TGF-α(16.8 ng/g) and IGF-1(3.36 μg/g) was found in the LBC of the first day postpartum, then rapidly decreased within 7 days, and the contents on the 7~(th) day were close to LM levels. The average content of EGF, TGF-α and IGF-1 in LBC within 4 days were 5.6 ng/g, 9.6 ng/g, 2.42 μg/g, which were 7.0, 13.7 and 6.1 times as that in LM. The contents of EGF, TGF-α in three bovine colostrums health-care food(BCHF) were significantly higher than two market milk powder and LM. The contents of EGF, TGF-α in prolactin were significantly higher than other two bovine colostrum health food. The content of IGF-1 in prolactin was 1.11 μg/g, which was significantly higher than that in LM.[Conclusion] The research could provide theoretical and experimental basis for the further development and utilization of bovine colostrum products.
引文
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[19] 崔娜,梁琪,文鹏程,等.牛初乳与常乳的物化性质对比分析[J].食品工业科技,2013,34(9):368-372.
[20] SHIMODA N,TASHIRO T,YAMAMORI H,et al.Effects of growth hormone and insulin-like growth factor-1 on protein metabolism,gut morphology,and cell-mediated immunity in burned rats[J].Nutrition,1997,13(6):540-546.
[21] IACOPETTA B J,GRIEU F,HORISBERGER M,et al.Epidermal growth factor in human and bovine milk[J].Acta paediatrica,1992,81(4):287-291.
[22] 孙瑶,刘宁.不同乳中表皮生长因子含量的测定[J].黑龙江畜牧兽医,2008(5):105.
[23] XIAO X,XIONG A H,CHEN X,et al.Epidermal growth factor concentrations in human milk,cow’s milk and cow’s milk-based infant formulas[J].Chinese medical journal,2002,115(3):451-454.
[24] 雷飞艳,云丹,王毕妮,等.加工方式对羊乳表皮生长因子(EGF)浓度的影响[J].食品与发酵工业,2017,43(3):186-190.
[2] URUAKPA F O,ISMOND M A H,AKOBUNDU E N T.Colostrum and its benefits:A review[J].Nutrition research,2002,22(6):755-767.
[3] BLUM J W,HAMMON H.Colostrum effects on the gastrointestinal tract,and on nutritional,endocrine and metabolic parameters in neonatal calves[J].Livestock production science,2000,66(2):151-159.
[4] POULIOT Y,GAUTHIER S F.Milk growth factors as health products:Some technological aspects[J].International dairy journal,2006,16(11):1415-1420.
[5] BLUM J W,BAUMRUCKER C R.Insulin-like growth factors(IGFs),IGF binding proteins,and other endocrine factors in milk:Role in the Newborn[J].Advances in experimental medicine and biology,2008,606:397-422.
[6] 云振宇,张烈兵,张和平.牛初乳类胰岛素生长因子-I治疗大鼠高血糖作用及其机理的研究[J].中国食品学报,2007,7(5):18-23.
[7] BALLARD F J,GROBOVAC S,NICHOLAS K R,et al.Differential changes the milk concentrations of epidermal growth factor and insulin-like growth factor-I during lactation in the tammar wallaby,Macropus eugenii[J].General and comparative endocrinology,1995,98(3):262-268.
[8] SHINNICHI O,HIDETOSHI S,TATSUROU S,et al.Insulin-like growth factor-I,GH,insulin and glucagon concentrations in bovine colostrum and in plasma of dairy cows and neonatal calves around parturition[J].Comparative biochemistry and physiology part A:Physiology,1989,94(4):805-808.
[9] 刘海燕,潘道东.免疫初乳及过渡乳中类胰岛素生长因子-I含量测定及其变化[J].食品工业科技,2009,30(2):117-118,122.
[10] 许愿,王希彪,崔世泉,等.民猪与长白猪初乳EGF、IGF-1和乳糖含量的比较研究[J].黑龙江畜牧兽医,2015(1):93-95.
[11] 秦宜德,罗欣,黄登莲,等.初乳中酶活性及生物活性因子含量的动态变化及其意义[J].中华妇产科杂志,2004,39(7):449-452.
[12] 王卉.转化生长因子α 对LoVo 细胞增殖及其它生物学功能影响的研究[D].哈尔滨:东北农业大学,2008:10-44.
[13] 马玉敏,王恬.初乳中生长因子对肠道生长发育的影响[J].动物科学与动物医学,1999,16(3):14-16.
[14] PLAYFORD R J.Peptide therapy and the gastroenterologist:Colostrum and milk-derived growth factors[J].Clinical nutrition,2001,20(1):101-106.
[15] ELFSTRAND L,LINDMARK-M? NSSON H,PAULSSON M,et al.Immunoglobulins,growth factors and growth hormone in bovine colostrum and the effects of processing[J].International dairy journal,2002,12(11):879-887.
[16] PAKKANEN R,AALTO J.Growth factors and antimicrobial factors of bovine colostrum[J].International dairy journal,1997,7(5):285-297.
[17] NIKOLIC I,STOJANOVIC I,VUJICIC M,et al.Standardized bovine colostrum derivative impedes development of type 1 diabetes in rodents[J].Immunobiology,2016,222(2):272-279.
[18] 王小军,高睿嘉,郁桦,等.牛初乳冻干粉和常乳冻干粉的降血糖及抗氧化作用研究[J].食品工业科技,2017,38(23):262-267,274.
[19] 崔娜,梁琪,文鹏程,等.牛初乳与常乳的物化性质对比分析[J].食品工业科技,2013,34(9):368-372.
[20] SHIMODA N,TASHIRO T,YAMAMORI H,et al.Effects of growth hormone and insulin-like growth factor-1 on protein metabolism,gut morphology,and cell-mediated immunity in burned rats[J].Nutrition,1997,13(6):540-546.
[21] IACOPETTA B J,GRIEU F,HORISBERGER M,et al.Epidermal growth factor in human and bovine milk[J].Acta paediatrica,1992,81(4):287-291.
[22] 孙瑶,刘宁.不同乳中表皮生长因子含量的测定[J].黑龙江畜牧兽医,2008(5):105.
[23] XIAO X,XIONG A H,CHEN X,et al.Epidermal growth factor concentrations in human milk,cow’s milk and cow’s milk-based infant formulas[J].Chinese medical journal,2002,115(3):451-454.
[24] 雷飞艳,云丹,王毕妮,等.加工方式对羊乳表皮生长因子(EGF)浓度的影响[J].食品与发酵工业,2017,43(3):186-190.