进口沉香挥发性成分的HS-GC-MS分析
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摘要
采用顶空气相色谱-质谱联用(HS-GC-MS)技术对产自5个国家(加里曼丹、马来西亚、孟加拉国、印度、印度尼西亚)的10个沉香样品的挥发性成分进行分析.结果表明,10个沉香样品的挥发性成分主要是芳香族类和倍半萜类化合物,相同产地但形态不同的沉香样品的挥发性组分种类和相对含量均存在一定差异,不同产地沉香样品的挥发性组分也呈现不同的差异,甘香烯、(+)-环苜蓿烯只在马来西亚沉香样品中检出,可作为该产地沉香的特征性成分;α-古芸烯和β-愈创木烯只在印度尼西亚沉香样品中检出,可作为该产地沉香的特征性成分;(+)-α-长叶蒎烯和α-荜澄茄油烯只在印度沉香样品中检出,可作为该产地沉香的特征性成分;产自印度尼西亚的沉香样品中醇类的种类和含量明显低于其他产地.该研究结果为沉香挥发性成分的快速检测和产地鉴别提供了新的思路.
The volatile components of 10 agarwood samples from 5 countries( Kalimantan,Malaysia,Bangladesh,India,Indonesia) were analyzed by headspace gas chromatography-mass spectrometry( HS-GC-MS).The results showed that the volatile components of 10 agarwood samples were mainly aromatic and sesquiterpenoids. The types and relative contents of volatile components of agarwood samples with the same origin but different morphology were different. The volatile components of the samples also showed certain differences. The mannene and(+)-cyclodecene were only detected in the agarwood samples of Malaysia and could be used as the characteristic components of the agarwood in the producing area; α-cluene and β-Guaiacrox were only detected in Indonesian agarwood samples and could be used as the characteristic component of the agarwood in this area;(+)-α-long-leafene and α-carboene were only detected in Indian agarwood samples and could be used as the characteristic component of agarwood in this area; the types and contents of alcohols in agarwood samples from Indonesia were significantly lower than those in other places. The results could provide a new idea for the rapid detection and identification of the agarwood production area.
The volatile components of 10 agarwood samples from 5 countries( Kalimantan,Malaysia,Bangladesh,India,Indonesia) were analyzed by headspace gas chromatography-mass spectrometry( HS-GC-MS).The results showed that the volatile components of 10 agarwood samples were mainly aromatic and sesquiterpenoids. The types and relative contents of volatile components of agarwood samples with the same origin but different morphology were different. The volatile components of the samples also showed certain differences. The mannene and(+)-cyclodecene were only detected in the agarwood samples of Malaysia and could be used as the characteristic components of the agarwood in the producing area; α-cluene and β-Guaiacrox were only detected in Indonesian agarwood samples and could be used as the characteristic component of the agarwood in this area;(+)-α-long-leafene and α-carboene were only detected in Indian agarwood samples and could be used as the characteristic component of agarwood in this area; the types and contents of alcohols in agarwood samples from Indonesia were significantly lower than those in other places. The results could provide a new idea for the rapid detection and identification of the agarwood production area.
引文
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[2]刘军民,徐鸿华.国产沉香研究进展[J].中药材,2005,28(7):627.
[3]江向东,粟忠蓉.沉香质量的快速分析[J].中药材,1996,19(12):632.
[4]刘玉峰,杨秀伟,刘铜华.沉香叶挥发油化学成分的GC-MS分析[J].中国现代中药,2007,9(8):7.
[5]梅文莉,曾艳波,刘俊,等.五批国产沉香挥发性成分的GC-MS分析[J].中药材,2007,30(5):551.
[6]戚树源,何厚才.激素对白木香悬浮培养细胞中苄基丙酮形成的影响[J].中药材,2001,24(5):318.
[7]梅文莉,曾艳波,刘俊,等.五批国产沉香挥发性成分的GC-MS分析[J].中药材,2007,30(5):551.
[8]郭晓玲,田佳佳,高晓霞,等.不同产区沉香药材挥发油成分GC-MS分析[J].中药材,2009,32(9):1354.
[9]田佳佳,郭晓玲,赖林城,等.国产沉香醇浸膏挥发油成分分析[J].时珍国医国药,2009,20(10):2505.
[10]刘宝峰,高丰展,房强,等.间接顶空固相微萃取-气相色谱-质谱法对红花香雪兰天然花香成分的分析[J].分析化学,2016,44(3):444.
[11]段宙位,李维国,窦志浩,等.沉香叶黄酮类化合物的提取及其抗氧化活性[J].食品科学,2015,36(6):45.
[12]沈汝青,陆麒,朱宝璋.超临界CO2沉香萃取及高级精油精制工艺[J].化工设计通讯,2018,44(1):126.
[13]刘明石,崔玮琪,金元宝,等.超临界CO2萃取沉香叶挥发油工艺条件优化研究[J].中国酿造,2014,33(2):63.
[14]王国庆,彩倩杰,刘云,等.顶空-气相色谱-质谱法测定改性聚硫橡胶中挥发性成分[J].郑州轻工业学院学报(自然科学版),2015,30(2):1.