GC-MS结合化学计量学解析法分析槐米中挥发油成分
|
摘要
通过水蒸气蒸馏法提取槐米中挥发油成分。利用GC-MS对其分离检测,结合化学计量学解析法(CRM)分辨重叠色谱峰。共分辨出46个色谱峰,鉴定了其中32个组分,占槐米挥发油总量的96. 35%。其中主要化学成分为8-十七碳烯(16. 30%)、雪松烯(12. 65%)、石竹烯(7. 95%)、醋酸百里酚酯(7. 63%)、棕榈酸(6. 79%)和优香芹酮(6. 68%)等成分。而其它26个组分只占38. 35%。因此,结合化学计量学解析法分析槐米挥发油化学成分,比单独使用GC-MS其结果更加准确、可靠。
The essential oil components in Flos Sophorae were extracted by steam distillation. gas chromatographymass spectrometry( GC-MS) combined with chemometric resolution method( CRM),which was a useful chemometric resolution method to resolve overlapping peaks in chromatogram,was employed to identify and comparatively analyze the volatile constituents. A total of 46 chromatographic peaks were resolved,and 32 components in the essential oils of Flos Sophorae were identified,which accounted for 96. 35% of the total relative contents. The main chemical compositions in the studied sample were 8-heptadecene( 16. 30%),cedrene( 12. 65%),caryophyllene( 7. 95%),thyme acetate( 7. 63%),hexadecanoic acid( 6. 79%) and eucarvone( 6. 68%),etc. And the other 26 constituents accounted for about 38. 35% of the total contents. It was demonstrated that CRM could provide a complementary and convenient method for accurate analysis of Flos Sophorae essential oils.
The essential oil components in Flos Sophorae were extracted by steam distillation. gas chromatographymass spectrometry( GC-MS) combined with chemometric resolution method( CRM),which was a useful chemometric resolution method to resolve overlapping peaks in chromatogram,was employed to identify and comparatively analyze the volatile constituents. A total of 46 chromatographic peaks were resolved,and 32 components in the essential oils of Flos Sophorae were identified,which accounted for 96. 35% of the total relative contents. The main chemical compositions in the studied sample were 8-heptadecene( 16. 30%),cedrene( 12. 65%),caryophyllene( 7. 95%),thyme acetate( 7. 63%),hexadecanoic acid( 6. 79%) and eucarvone( 6. 68%),etc. And the other 26 constituents accounted for about 38. 35% of the total contents. It was demonstrated that CRM could provide a complementary and convenient method for accurate analysis of Flos Sophorae essential oils.
引文
[1]王锦鸿,陈仁寿.临床实用中药辞典[M].北京:金盾出版社,2003:796-797.
[2]黄海兰,李俊,徐波.槐米抗食用油脂氧化活性及其成分研究[J].食品科学,2007,28(8):86-89.
[3]杨海霞,夏新奎,陈利军.槐米挥发油化学成分GC-MS分析[J].南阳师范学院学报,2010,9(3):38-39.
[4]李杰红,陈代武.固相微萃取-气相色谱-质谱技术分析槐花的挥发性成分[J].化学研究,2007,18(1):77-79.
[5]梁逸曾.白灰黑复杂多组分体系及其化学计量学算法[M].长沙:湖南科学技术出版社,1996:177-206.
[6] He L J,Liang Y Z,et al. GC-MS Study on Chemical Constituents of Essential Oil of Lemiaceae Plants[J]. Acta Chimica Sinica,2007,65(3):227-232.
[7]佘金明,梁逸曾,彭友林,等.药对白术-茅苍术及其单味药中挥发油成分的比较分析[J].分析化学,2010,38(9):1282-1286.
[8]佘金明,钟明,梁逸曾,等.直观推导式演进特征投影法辅助GCMS分析桂枝中的挥发油[J].华西药学杂志,2010,25(4):446-449.
[9] Liang Y Z,Kvalheim O M,Keller H R,et al. Heuristic evolving latent projections:resolving two-way multicomponent data Part 2:Detection and resolution of minor constituents[J]. Anal Chem,1992,64(8):946-953.
[10] Kvalheim O M,Liang Y Z. Heuristic evolving latent projections:resolving two-way multicomponent data Part 1:Selectivity, latent projective graph,datascope,local rank and unique resolution[J]. Anal Chem,1992,64(8):936-946.
[11]赵晨曦,曾映旭,李彬,等.铁线莲属植物挥发成分的GC-MS研究[J].分析测试学报,2007,26(5):172-175.
[2]黄海兰,李俊,徐波.槐米抗食用油脂氧化活性及其成分研究[J].食品科学,2007,28(8):86-89.
[3]杨海霞,夏新奎,陈利军.槐米挥发油化学成分GC-MS分析[J].南阳师范学院学报,2010,9(3):38-39.
[4]李杰红,陈代武.固相微萃取-气相色谱-质谱技术分析槐花的挥发性成分[J].化学研究,2007,18(1):77-79.
[5]梁逸曾.白灰黑复杂多组分体系及其化学计量学算法[M].长沙:湖南科学技术出版社,1996:177-206.
[6] He L J,Liang Y Z,et al. GC-MS Study on Chemical Constituents of Essential Oil of Lemiaceae Plants[J]. Acta Chimica Sinica,2007,65(3):227-232.
[7]佘金明,梁逸曾,彭友林,等.药对白术-茅苍术及其单味药中挥发油成分的比较分析[J].分析化学,2010,38(9):1282-1286.
[8]佘金明,钟明,梁逸曾,等.直观推导式演进特征投影法辅助GCMS分析桂枝中的挥发油[J].华西药学杂志,2010,25(4):446-449.
[9] Liang Y Z,Kvalheim O M,Keller H R,et al. Heuristic evolving latent projections:resolving two-way multicomponent data Part 2:Detection and resolution of minor constituents[J]. Anal Chem,1992,64(8):946-953.
[10] Kvalheim O M,Liang Y Z. Heuristic evolving latent projections:resolving two-way multicomponent data Part 1:Selectivity, latent projective graph,datascope,local rank and unique resolution[J]. Anal Chem,1992,64(8):936-946.
[11]赵晨曦,曾映旭,李彬,等.铁线莲属植物挥发成分的GC-MS研究[J].分析测试学报,2007,26(5):172-175.