芪术痹湿颗粒中黄芪检验方法的建立
|
摘要
目的建立芪术痹湿颗粒中黄芪检验鉴别和测定方法。方法分别采用薄层色谱、高效液相色谱-蒸发光散射(ELSD)法鉴别测定处方中的黄芪、黄芪中黄芪甲苷的含量。结果黄芪薄层色谱具有较强的专属性,且显示斑点清晰,该测定方法具有良好的稳定性、精密度以及重复性,平均加样回收率为99.00%,RSD为1.27%(9例)。结论该检验测定方法操作简单方便,可用于芪术痹湿颗粒的质量控制中。
Objective To establish the method for identification and determination of Astragalus membranaceus in Qizhu Bishi granules.Methods The content of Astragalus membranaceus in Radix Astragali and Astragalus membranaceus in the prescription was determined by thin layer chromatography and high performance liquid chromatography-ELSD. Results The TLC of Astragalus membranaceus has strong specificity and shows clear spots. The method has good stability,precision and reproducibility. The average recovery rate is 99%,and RSD is 1. 27%( n = 9). Conclusion The test method is easy to operate and can be used as the quality control of Qizhu Bishi granules.
Objective To establish the method for identification and determination of Astragalus membranaceus in Qizhu Bishi granules.Methods The content of Astragalus membranaceus in Radix Astragali and Astragalus membranaceus in the prescription was determined by thin layer chromatography and high performance liquid chromatography-ELSD. Results The TLC of Astragalus membranaceus has strong specificity and shows clear spots. The method has good stability,precision and reproducibility. The average recovery rate is 99%,and RSD is 1. 27%( n = 9). Conclusion The test method is easy to operate and can be used as the quality control of Qizhu Bishi granules.
引文
[1]吴怡,邹桂欣,王光函,等.化胃舒颗粒供试品制备方法对其黄芪甲苷含量的影响[J].中国药师,2014,17(1):29-32.
[2]李晓强,陈克玲.探究归芪参胶囊功效成分及检验方法[J].临床医药文献电子杂志,2017,4(31):6099.
[3]丁玲,刘丽芳,吴世婷,等.黄芪解毒汤含药血清对乳腺癌细胞MCF-7中SHH、PTCH1、SMO、GLI1基因及Cyclin D1蛋白表达的影响[J].中医药导报,2016,23(24):23-28.
[4]吴佳琪,张渊,丁越,等.HPLC-ELSD法测定益气祛白颗粒中黄芪甲苷的含量[J].中国临床药学杂志,2014,23(2):106-108.
[5]郭琪,李向阳,宋汉敏.小儿健脾散中黄芪甲苷、欧前胡素和异欧前胡素的含量测定方法探索[J].中医研究,2014,27(9):71-75.
[6]许崇花,王洪新,唐富天,等.黄芪甲苷对异丙肾上腺素诱导大鼠主动脉氧化应激和炎症的影响[J].中药药理与临床,2015,31(1):50-54.
[2]李晓强,陈克玲.探究归芪参胶囊功效成分及检验方法[J].临床医药文献电子杂志,2017,4(31):6099.
[3]丁玲,刘丽芳,吴世婷,等.黄芪解毒汤含药血清对乳腺癌细胞MCF-7中SHH、PTCH1、SMO、GLI1基因及Cyclin D1蛋白表达的影响[J].中医药导报,2016,23(24):23-28.
[4]吴佳琪,张渊,丁越,等.HPLC-ELSD法测定益气祛白颗粒中黄芪甲苷的含量[J].中国临床药学杂志,2014,23(2):106-108.
[5]郭琪,李向阳,宋汉敏.小儿健脾散中黄芪甲苷、欧前胡素和异欧前胡素的含量测定方法探索[J].中医研究,2014,27(9):71-75.
[6]许崇花,王洪新,唐富天,等.黄芪甲苷对异丙肾上腺素诱导大鼠主动脉氧化应激和炎症的影响[J].中药药理与临床,2015,31(1):50-54.