地蜂子免疫作用的药理研究
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摘要
目的探讨地蜂子的免疫作用,为地蜂子的临床应用和新用途开发提供思路。方法采用碳粒廓清实验法测定小鼠网状内皮系统中的单核巨噬细胞功能及其对小鼠免疫器官的影响,应用DNCB测试小鼠迟发型超敏反应。结果地蜂子水提液低剂组、醇提液高剂组脾脏和胸腺的重量均高于空白对照组,差异具有统计学意义(P<0.05);地蜂子水提液低剂组、醇提液高剂组的小鼠的碳粒廓清指数K和吞噬指数α高于空白对照组,差异具有统计学意义(P<0.05)。地蜂子水提液、醇提液均能明显抑制DNCB引起的小鼠迟发型超敏反应,差异具有统计学意义(P <0.05)。结论地蜂子能增加小鼠免疫器官的重量,增强其单核巨噬细胞的吞噬功能,具有明显的免疫调节作用。
Objective To explore the immune function of terrestrial wasps, and to provide ideas for clinical application and new use development of terrestrial wasps. Methods Carbon particle clearance test was used to determine the function of monocytes and macrophages in the reticuloendothelial system of mice and their effects on the immune organs of mice. DNCB was used to test the delayed hypersensitivity of mice. ResultsThe weight of spleen and thymus in the low-dose group and the high-dose group were higher than those in the blank control group(P < 0.05). The carbon clearance index K and phagocytosis index α in the low-dose group and the high-dose group were higher than those in the blank control group(P < 0.05). The water extract and alcohol extract of Fructus Honeybee could significantly inhibit the delayed hypersensitivity induced by DNCB in mice, the difference was statistically significant(P < 0.05). Conclusion The bee can increase the weight of immune organs and enhance the phagocytosis of mononuclear macrophages in mice, which has obvious immunomodulatory effect.
Objective To explore the immune function of terrestrial wasps, and to provide ideas for clinical application and new use development of terrestrial wasps. Methods Carbon particle clearance test was used to determine the function of monocytes and macrophages in the reticuloendothelial system of mice and their effects on the immune organs of mice. DNCB was used to test the delayed hypersensitivity of mice. ResultsThe weight of spleen and thymus in the low-dose group and the high-dose group were higher than those in the blank control group(P < 0.05). The carbon clearance index K and phagocytosis index α in the low-dose group and the high-dose group were higher than those in the blank control group(P < 0.05). The water extract and alcohol extract of Fructus Honeybee could significantly inhibit the delayed hypersensitivity induced by DNCB in mice, the difference was statistically significant(P < 0.05). Conclusion The bee can increase the weight of immune organs and enhance the phagocytosis of mononuclear macrophages in mice, which has obvious immunomodulatory effect.
引文
[1]陈会玲,闫斌,覃祝,等.地蜂子化学成分的研究[J].中成药,2015,37(12):2674-2677.
[2]覃祝.地蜂子体外抗HSV-1病毒活性及其作用机理的初步研究[D].武汉:湖北中医药大学,2016:5.
[3]杨芙蓉,王平,曾兰,等.从脾肾归经角度探析《本草纲目》轻身延年药物[J].时珍国医国药,2018,29(1):223-225.
[4]李梦婷,胡婷婷,熊秋韵,等.炮天雄和刨附片对小鼠免疫功能的影响[J].中药与临床,2017,8(3):45-48.
[5]于浩然,陈晓宇,田振坤.小鼠体内五味子乙素抗炎和增强免疫功能研究[J].中国药师,2018,21(6):973-976.
[6]孟莉,向绍杰,刘小虎,等.化胃舒颗粒对DNCB致小鼠迟发型超敏反应模型的影响[J].中国医药科学,2018,8(13):49-51.
[7]张君,徐志立,张振秋.车前子胶对免疫功能低下小鼠免疫功能的影响[J].辽宁中医杂志,2018,45(9):1965-1967.
[8]李娜娜,王雯颍,李承平,等.残黄片对免疫低下小鼠免疫调节作用的研究[J].时珍国医国药,2018,29(5):1088-1090.
[9]林慧,梅全喜.蛇黄肝炎汤含药血清对免疫抑制大鼠免疫功能的调节作用[J].中国医院药学杂志,2016,36(20):1763-1766.
[10]陈艳,殷洪明.免疫功能与超敏C反应蛋白检验对小儿支原体肺炎的临床价值分析[J].临床合理用药,2018,11(9A):156-157.
[11]刘波.健脾益肺祛痰方对支原体肺炎患儿炎症因子及免疫功能的影响[J].中国中医药科技,2015,22(3):250-251.
[12]杨喜顺,陈金强,刘启乐,等.免疫功能与超敏C反应蛋白检验对小儿肺炎支原体肺炎的临床意义[J].中国实用医药,2017,12(12):12-14.
[2]覃祝.地蜂子体外抗HSV-1病毒活性及其作用机理的初步研究[D].武汉:湖北中医药大学,2016:5.
[3]杨芙蓉,王平,曾兰,等.从脾肾归经角度探析《本草纲目》轻身延年药物[J].时珍国医国药,2018,29(1):223-225.
[4]李梦婷,胡婷婷,熊秋韵,等.炮天雄和刨附片对小鼠免疫功能的影响[J].中药与临床,2017,8(3):45-48.
[5]于浩然,陈晓宇,田振坤.小鼠体内五味子乙素抗炎和增强免疫功能研究[J].中国药师,2018,21(6):973-976.
[6]孟莉,向绍杰,刘小虎,等.化胃舒颗粒对DNCB致小鼠迟发型超敏反应模型的影响[J].中国医药科学,2018,8(13):49-51.
[7]张君,徐志立,张振秋.车前子胶对免疫功能低下小鼠免疫功能的影响[J].辽宁中医杂志,2018,45(9):1965-1967.
[8]李娜娜,王雯颍,李承平,等.残黄片对免疫低下小鼠免疫调节作用的研究[J].时珍国医国药,2018,29(5):1088-1090.
[9]林慧,梅全喜.蛇黄肝炎汤含药血清对免疫抑制大鼠免疫功能的调节作用[J].中国医院药学杂志,2016,36(20):1763-1766.
[10]陈艳,殷洪明.免疫功能与超敏C反应蛋白检验对小儿支原体肺炎的临床价值分析[J].临床合理用药,2018,11(9A):156-157.
[11]刘波.健脾益肺祛痰方对支原体肺炎患儿炎症因子及免疫功能的影响[J].中国中医药科技,2015,22(3):250-251.
[12]杨喜顺,陈金强,刘启乐,等.免疫功能与超敏C反应蛋白检验对小儿肺炎支原体肺炎的临床意义[J].中国实用医药,2017,12(12):12-14.