挑刺膏肓穴对慢性疲劳综合征大鼠血清抗氧化指标和细胞因子的影响
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摘要
目的:通过观察挑刺膏肓穴对慢性疲劳综合征(CFS)大鼠血清抗氧化指标超氧化物歧化酶(SOD)活力、谷胱甘肽过氧化物酶(GSH-Px)活力、丙二醛(MDA)及细胞因子白细胞介素-1β(IL-1β)、干扰素-γ(IFN-γ)、肿瘤坏死因子(TNF-α)的影响,研究挑刺膏肓穴治疗CFS的作用机制。方法:按随机数字表将30只健康SD大鼠随机分成正常组、模型组、挑刺组,每组10只。采用慢性束缚和强制冷水游泳的方法制备CFS模型大鼠。挑刺组给予挑刺膏肓穴治疗,正常组与模型组不做治疗,但按相同方式抓取。挑刺每2天治疗1次,1周治疗3次。治疗1周后检测各组大鼠血清SOD、GSH-Px活力以及MDA、IL-1β、IFN-γ、TNF-α的含量。结果:慢性束缚加强制冷水游泳的复合应激可导致大鼠精神状态差及行为活动减少,挑刺膏肓穴可改善CFS大鼠的精神状态及行为活动。与正常组比较,模型组大鼠血清SOD活力、GSH-Px活力及IFN-γ、TNF-α含量均明显降低(P <0. 05),MDA、IL-1β含量显著升高(P <0. 05);挑刺组大鼠血清SOD、GSH-Px活力及IFN-γ、TNF-α含量显著高于模型组(P <0. 05),MDA、IL-1β含量明显低于模型组(P <0. 05)。结论:该研究表明挑刺膏肓穴能调节CFS模型大鼠血清SOD、GSH-Px活力及MDA、IL-1β、IFN-γ、TNF-α的含量,抗氧化作用、抑制炎症反应及调节免疫功能可能是挑刺治疗CFS的机制之一。
Objective: To observe immunomodulating and antioxidant effects of needle-pricking at Gaohuang( BL42) on rats with chronic fatigue syndrome. Methods: A total of 30 male SD rats were randomly divided into control group,model group and needle-pricking group. CFS model was established by bounding and swimming in cold for 14 days. Rats in the needle-pricking group were given needle-pricking at Gaohuang( BL43),once two days for 7 days. The activities of SOD,GSH-Px and the levels of MDA,IL-1β,IFN-γ and TNF-α in serum were elevated. Results: Compared with those in the control group,the activities of serum SOD and GSH-Px and the contents of IFN-γ and TNF-α in the model group significantly lower( P < 0. 05). The contents of MDA and IL-1β in serum were significantly higher( P < 0. 05). The activities of serum SOD,GSH-Px and the contents of IFN-γ and TNF-α were obviously increased in the needle-pricking group( P < 0. 05) and the levels of serum MDA and IL-1β in the needle-pricking groups were significantly lower( P < 0. 05) compared with those of the model group. Conclusion: The needle-pricking therapy can improve the antioxidant activity and immunoregulation of CFS rats,which might be one of the mechanisms.
Objective: To observe immunomodulating and antioxidant effects of needle-pricking at Gaohuang( BL42) on rats with chronic fatigue syndrome. Methods: A total of 30 male SD rats were randomly divided into control group,model group and needle-pricking group. CFS model was established by bounding and swimming in cold for 14 days. Rats in the needle-pricking group were given needle-pricking at Gaohuang( BL43),once two days for 7 days. The activities of SOD,GSH-Px and the levels of MDA,IL-1β,IFN-γ and TNF-α in serum were elevated. Results: Compared with those in the control group,the activities of serum SOD and GSH-Px and the contents of IFN-γ and TNF-α in the model group significantly lower( P < 0. 05). The contents of MDA and IL-1β in serum were significantly higher( P < 0. 05). The activities of serum SOD,GSH-Px and the contents of IFN-γ and TNF-α were obviously increased in the needle-pricking group( P < 0. 05) and the levels of serum MDA and IL-1β in the needle-pricking groups were significantly lower( P < 0. 05) compared with those of the model group. Conclusion: The needle-pricking therapy can improve the antioxidant activity and immunoregulation of CFS rats,which might be one of the mechanisms.
引文
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[3]李莉,卢洪洲.慢性疲劳综合征研究进展[J].内科理论与实践,2009,4(4):331-333.
[4]衰萍,梁伯衡.慢性疲劳综合征的流行病学特征[J].国外医学卫生学分册,2003,30(2):70-74.
[5]王京京,宋玉静,吴中朝,等.针灸治疗慢性疲劳综合征随机对照试验的Meta分析[J].针刺研究,2009,34(6):421-428.
[6]王京京,宋玉静,吴中朝,等.针刺对慢性疲劳综合征患者生存质量影响:随机对照研究[J].中国针灸,2009,29(10):780-784.
[7]陈兴华,黎璐茜,章闻,等.针刺治疗慢性疲劳综合征随机对照研究[J].中国针灸,2010,30(7):533-536.
[8]郑盛惠,郑生智,焦建凯,等.俞募配穴针灸治疗慢性疲劳综合征的临床随机对照研究[J].辽宁中医杂志,2012,39(4):726-728.
[9]王天芳,陈易新,季绍良,等.慢性束缚致慢性疲劳动物模型的研制及其行为学观察[J].中国中医基础医学杂志,1999,5(5):25.
[10]尹喜玲,肖颖,李可基.多重应激建立慢性疲劳综合征动物模型的研究[J].中国运动医学杂志,2005,24(4):452-456.
[11]张露芬.实验针灸学[M].北京:化学工业出版社,2010.
[12]梁庆临,黎文献.针挑疗法[M].广州:广东科技出版社,2010.
[13]林国华,裴文娅,李茜,李晶晶,张家维.张家维教授挑针疗法临床应用举隅[J].新中医,2016,48(5):280-282.
[14]许云祥,陈贵珍,张家维.张家维教授皮部理论临床应用经验[J].针灸临床杂志,2007,23(1):44-45.
[15]王小峰.慢性疲劳综合征病因病机探讨[J].辽宁中医药大学学报,2012,14(7):140-142.
[16]田德禄.中医内科学[M].北京:中国中医药出版社,2003:218.
[17]洪肖娟,吴曦,冉茜.性疲劳综合征动物模型制作与检测[J].辽宁中医杂志,2011,38(4):737-739.
[18]王芳,姚堃.β-干扰素及其重组产品的基础和临床应用[J].微生物学免疫学进展,2005,33(2):50.
[19]侯云德,吴淑华.干扰素[M].北京:人民卫生出版社,1982:70-79.
[20]谢丛华.肿瘤坏死因子的多重临床意义[J].国外医学·内科学分册,2005,32(6):247.
[21]Sofia Xanthoulea,Manolis Pasparakis,Stavroula Kousteni,et al.Tumor necrosis factor(TNF)receptor shedding controls thresholds of innate immune activation that balance opposing TNF functions in infectious and inflammatory diseases[J].J Exp Med,2004,200(3):367.
[22]Ren K,Torres R.Role of interleukin-1βduring pain and inflammation[J].Brain Res Rev,2009,60(1):57-64.
[23]Samad TA,Moore KA,Sapirstein A,et al.Interleukin-1βmediated induction of Cox-2 in the CNS contributes to inflammatory pain hypersensitivity[J].Nature,2001,410(6827):471-475.
[24]赵克然.氧自由基与临床[M].台北:合记图书出版社,2003:232.
[25]李忠仁.针灸抗氧应激效应及机理[J].南京国际中医药论坛暨第二届世界中医药大会,2004:43-49.
[26]Valavanidis A,Vlahogianni T,Dassenakis M,et al.Molecular biomarkers of oxidative stress in aquatic organisms in relation to toxic environmental pollutants[J].Ecotoxicol Environ Saf,2006,64(2):178-189.