藏药匙叶翼首草的快繁技术研究
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摘要
目的:建立藏药匙叶翼首草组培快速繁殖体系。方法:采用不同浓度的植物生长调节剂诱导产生丛生芽与根,并筛选三种不同栽培基质。结果:最好的消毒条件为75%酒精处理30 s+2.5%Na Cl O处理2.5 min+MS液(含2.5%PPM抗菌剂)处理3小时。诱导丛生芽的最佳培养基为MS+6BA 3 mg·L-1+NAA 0.5 mg·L-1,其次为MS+6BA 3 mg·L-1+NAA 0.2 mg·L-1。丛生芽生根的适宜培养基为MS+NAA 0.2 mg·L-1,小苗最适宜栽培基质为蛭石:珍珠岩=1∶1。结论:通过本研究的方法可得到翼首草无菌苗与再生苗。
Objective: In order to establish the tissue culture rapid propagation system of Tibetan medicine of Pterocephalus hookeri. Methods: The study took leaves as materials and adopted different concentration of plant growth regulators to induce cluster buds and roots,and then screened three different growing media. Results: The best disinfection conditions was for 30 s of 75 % alcohol,2. 5 min of 2. 5% Na Cl O,3 hours of MS liquid medium( containing 2. 5 % PPM). It suggested that the best medium for inducing cluster buds is MS + 6BA 3 mg·L- 1+ NAA 0. 5 mg·L- 1,and the second is MS + 6BA 3 mg·L- 1+ NAA 0. 2 mg·L- 1. The most suitable medium of inducing roots is MS + NAA 0. 2 mg·L- 1. The optimal cultivation substrate is vermiculite: perlite = 1 ∶ 1. Conclusion: The sterile seedling and regeneration plants can be obtained by the study.
Objective: In order to establish the tissue culture rapid propagation system of Tibetan medicine of Pterocephalus hookeri. Methods: The study took leaves as materials and adopted different concentration of plant growth regulators to induce cluster buds and roots,and then screened three different growing media. Results: The best disinfection conditions was for 30 s of 75 % alcohol,2. 5 min of 2. 5% Na Cl O,3 hours of MS liquid medium( containing 2. 5 % PPM). It suggested that the best medium for inducing cluster buds is MS + 6BA 3 mg·L- 1+ NAA 0. 5 mg·L- 1,and the second is MS + 6BA 3 mg·L- 1+ NAA 0. 2 mg·L- 1. The most suitable medium of inducing roots is MS + NAA 0. 2 mg·L- 1. The optimal cultivation substrate is vermiculite: perlite = 1 ∶ 1. Conclusion: The sterile seedling and regeneration plants can be obtained by the study.
引文
[1]庞伟.藏药翼首草的研究与应用[J].中国民族医药杂志,2007,13(5):63-65.
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[4]张雪梅,杨丰庆,夏之宁.藏药翼首草的药理作用及其质量评价研究进展[J].中国药房,2012,23(35):3356-3358.
[5]Guo C X,Wu Y C,Zhu Y Z,et al.In Vitro and In Vivo Antitumor Effects of n-Butanol Extracts of Pterocephalus hookeri on Hep3B Cancer Cell[J].Hindawi,2015:1-10.
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[7]杨敬军,马令法,何淑玲,等.豌豆苗浸出液对匙叶翼首草种子萌发特性的影响[J].广东农业科学,2013,40(22):42-44.
[8]常毓巍,何淑玲,杨敬军,等.不同叶面肥对匙叶翼首草生长、产量和品质的影响[J].贵州农业科学,2011,39(12):67-70.
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[11]张艺,李文军,孟宪丽.藏药翼首草化学成分的研究[J].成都中医药大学学报,2002,25(3):41-42,46.
[12]Graikou K,Aligiannis N,Chinou I B.Chemical constituents from Pterocephalus perennis subsp.perennis(Dipsacaceae)[J].Biochem Syst Ecol,2006,34(5):438-441.
[13]余鸽,龙凤来,黄时伟.环烯醚萜药理作用研究进展[J].陕西林业科技,2009(2):69-73.
[14]沈芃.藏药翼首草抗类风湿性关节炎作用与机理研究[D].成都:成都中医药大学,2002.
[15]张雪梅.藏药翼首草化学成分及质量评价方法研究[D].重庆:重庆大学,2013.
[16]王姝,赵玉桥,罗国坤,等.组织培养技术在药用植物中的应用研究进展[J].贵州农业科学,2010,38(12):26-29.
[2]国家药典委员会.中华人民共和国药典:一部[S].北京:中国医药科技出版社,2010:358.
[3]中国科学院中国植物志编辑委员会.中国植物志:第73(1)卷[M].北京:科学出版社,1986:69.
[4]张雪梅,杨丰庆,夏之宁.藏药翼首草的药理作用及其质量评价研究进展[J].中国药房,2012,23(35):3356-3358.
[5]Guo C X,Wu Y C,Zhu Y Z,et al.In Vitro and In Vivo Antitumor Effects of n-Butanol Extracts of Pterocephalus hookeri on Hep3B Cancer Cell[J].Hindawi,2015:1-10.
[6]杨敬军,马令法,何淑玲,等.匙叶翼首草种子特性及萌发最适条件的研究[J].西南农业学报,2013,26(6):2489-2492.
[7]杨敬军,马令法,何淑玲,等.豌豆苗浸出液对匙叶翼首草种子萌发特性的影响[J].广东农业科学,2013,40(22):42-44.
[8]常毓巍,何淑玲,杨敬军,等.不同叶面肥对匙叶翼首草生长、产量和品质的影响[J].贵州农业科学,2011,39(12):67-70.
[9]兰小中,周战.一种高海拔地区翼首草的高产人工栽培技术[P].CN:103141291A,2013-06-12.
[10]田军,吴凤锷,丘明华,等.匙叶翼首花的化学成分[J].天然产物研究与开发,2000,12(1):35-38.
[11]张艺,李文军,孟宪丽.藏药翼首草化学成分的研究[J].成都中医药大学学报,2002,25(3):41-42,46.
[12]Graikou K,Aligiannis N,Chinou I B.Chemical constituents from Pterocephalus perennis subsp.perennis(Dipsacaceae)[J].Biochem Syst Ecol,2006,34(5):438-441.
[13]余鸽,龙凤来,黄时伟.环烯醚萜药理作用研究进展[J].陕西林业科技,2009(2):69-73.
[14]沈芃.藏药翼首草抗类风湿性关节炎作用与机理研究[D].成都:成都中医药大学,2002.
[15]张雪梅.藏药翼首草化学成分及质量评价方法研究[D].重庆:重庆大学,2013.
[16]王姝,赵玉桥,罗国坤,等.组织培养技术在药用植物中的应用研究进展[J].贵州农业科学,2010,38(12):26-29.