无乳链球菌检测的影响因素及方法学改进
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摘要
目的:基于无乳链球菌(GBS)检测率不高的情况,对实验前后可能影响无乳链球菌检出率的相关因素进行分析,进而对各个操作环节的具体流程进行改进,使用具有与更高检出率的容器进行培养,促进GBS检出率的提高。方法:选取某院2017年1月1日～2017年12月31日之间采集的分泌物标本11067例为对照组,选取同时期的分泌物标本12822例为观察组,采用改进的操作流程采集照组患者的分泌物标本,采用采样方法标准化的操作流程采集观察组患者的分泌物标本。结果:对照组采集标本11067例,其中阳性255例,阴性10812例,阳性检出率为2.3%;观察组采集标本12822例,其中阳性1090例,阴性11732例,阳性检出率为8.5%。对照组明显低于观察组,差异有统计学意义(P<0.05)。结论:改进标本采集操作流程之后有助于提高无乳链球菌的阳性检出率,说明规范化的操作对提高GBS检出率有着重要的意义。
Objective: Based on the low detection rate of streptococcus agalactiae(GBS), the relevant factors that may affect the detection rate of streptococcus agalactiae before and after the experiment were analyzed, and then the specific process of each operation link was improved, with higher detection rate of container, thereby promoting the improvement of GBS detection rate. Methods: 11067 secretion specimens collected from January 1, 2017 to December 31, 2017 in a hospital were selected as the control group, and 12822 secretion specimens collected during the same period were selected as the observation group. The secretion samples of the observation group were collected by using the improved operation procedure, and the secretion samples of the observation group were collected by using the standardized operation procedure. Results: In the control group, 11067 samples were collected, including 255 positive samples and 10812 negative samples, with a positive detection rate of 2.3%. In the observation group, 12822 samples were collected, including 1090 positive samples and 11732 negative samples, with a positive detection rate of 8.5%. The control group was significantly lower than the observation group, the difference was statistically significant(P<0.05). Conclusion: it is helpful to improve the positive detection rate of lactobacillus streptococci after improving specimen collection operation procedure, indicating that the standardized operation is of great significance to improve GBS detection rate.
Objective: Based on the low detection rate of streptococcus agalactiae(GBS), the relevant factors that may affect the detection rate of streptococcus agalactiae before and after the experiment were analyzed, and then the specific process of each operation link was improved, with higher detection rate of container, thereby promoting the improvement of GBS detection rate. Methods: 11067 secretion specimens collected from January 1, 2017 to December 31, 2017 in a hospital were selected as the control group, and 12822 secretion specimens collected during the same period were selected as the observation group. The secretion samples of the observation group were collected by using the improved operation procedure, and the secretion samples of the observation group were collected by using the standardized operation procedure. Results: In the control group, 11067 samples were collected, including 255 positive samples and 10812 negative samples, with a positive detection rate of 2.3%. In the observation group, 12822 samples were collected, including 1090 positive samples and 11732 negative samples, with a positive detection rate of 8.5%. The control group was significantly lower than the observation group, the difference was statistically significant(P<0.05). Conclusion: it is helpful to improve the positive detection rate of lactobacillus streptococci after improving specimen collection operation procedure, indicating that the standardized operation is of great significance to improve GBS detection rate.
引文
1 李相新,郑芬,张玲华,等.女性生殖道B群链球菌显色培养法筛查与耐药性分析研究.中华医院感染学杂志,2015,25(19):4379～4381.
2 陆庭嫣,沈俐,唐振华.不同培养基及B族链球菌增菌肉汤检测B族链球菌效果评价.检验医学,2015,30(9):890～893.
3 王丽,叶巍,马杰.实时荧光PCR技术和细菌培养法检测妊娠晚期孕妇定植B群链球菌临床分析.国际检验医学杂志,2014,35(16):2220～2221.
4 曾选.江西1019例孕妇生殖道B群链球菌感染情况分析.中国妇幼保健,2014,29(27):4457～4459.
5 彭捷,黄荣富,钟文,等.围产期孕妇泌尿生殖道无乳链球菌的感染与耐药性分析.中华医院感染学杂志,2017,27(8):1841～1844.
6 陈家慧,何振娟.乳铁蛋白和新生儿感染.临床儿科杂志,2013,31(3):299～300.
7 姚瑶,屠妍,芦起.新生儿败血症早期实验室诊断指标的价值评价.中国当代儿科杂志,2015,17(5):425～429.
8 林苗苗.新生儿重症肺炎伴心力衰竭临床诊治分析.中国妇幼保健,2017,28(29):4914～4915.
2 陆庭嫣,沈俐,唐振华.不同培养基及B族链球菌增菌肉汤检测B族链球菌效果评价.检验医学,2015,30(9):890～893.
3 王丽,叶巍,马杰.实时荧光PCR技术和细菌培养法检测妊娠晚期孕妇定植B群链球菌临床分析.国际检验医学杂志,2014,35(16):2220～2221.
4 曾选.江西1019例孕妇生殖道B群链球菌感染情况分析.中国妇幼保健,2014,29(27):4457～4459.
5 彭捷,黄荣富,钟文,等.围产期孕妇泌尿生殖道无乳链球菌的感染与耐药性分析.中华医院感染学杂志,2017,27(8):1841～1844.
6 陈家慧,何振娟.乳铁蛋白和新生儿感染.临床儿科杂志,2013,31(3):299～300.
7 姚瑶,屠妍,芦起.新生儿败血症早期实验室诊断指标的价值评价.中国当代儿科杂志,2015,17(5):425～429.
8 林苗苗.新生儿重症肺炎伴心力衰竭临床诊治分析.中国妇幼保健,2017,28(29):4914～4915.