三种不同来源的防风种子活力研究
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摘要
目的为了测定防风种子的千粒重、生活力和发芽率等指标来评价3种不同来源防风种子的质量优劣情况。方法试验采用四分法测定种子千粒重,四唑染色法测定种子生活力,用平铺滤纸法和倒扣滤纸法及脱脂棉法作为纸床的方法测定种子的发芽率。结果 3种种子千粒重大小依次为关防风>海拉尔防风>F_1代海拉尔驯化防风,通过数理统计分析得出关防风种子千粒重较优;通过生活力实验发现关防风的生活力在染色浓度为0.1%、0.5%、1.0%时均值均优于F_1代海拉尔驯化防风和海拉尔防风;关防风种子总发芽率与海拉尔防风及F_1代海拉尔驯化防风相比发芽率较高。结论关防风种子在3种种子中活力较优。
Objective In order to determine the thousand grain weight,viability and germination rate of windproof seeds,the quality of the windproof seeds from three different sources was evaluated.Methods The test used four-point method to determine the seed weight of seeds,the tetrazolium staining method was used to determine the seed viability,and the germination rate of the seeds was determined by the method of flat filter paper and inverted filter paper and cotton wool as paper bed.Results The results show that the seed thousand grain weight is Guan windproof>Hailar windproof>F_1 generation Hailar domesticated windproof.Through mathematical statistics analysis,it is concluded that the wind-proof seed has a thousand-grain weight;Through the viability experiment,it is found that the viability of the Guan windproof is better than the F_(1 )generation Hailar windproof and Hailar windproof when the dyeing concentration is 0.1%,0.5%,1.0%.According to the germination experiment,the total germination rate of the Guan windproofseeds is higher than that of the Hailaer windproof and the F_1 generation Hailar windproof.Conclusion Guan windproof seeds are more active in three seeds.
Objective In order to determine the thousand grain weight,viability and germination rate of windproof seeds,the quality of the windproof seeds from three different sources was evaluated.Methods The test used four-point method to determine the seed weight of seeds,the tetrazolium staining method was used to determine the seed viability,and the germination rate of the seeds was determined by the method of flat filter paper and inverted filter paper and cotton wool as paper bed.Results The results show that the seed thousand grain weight is Guan windproof>Hailar windproof>F_1 generation Hailar domesticated windproof.Through mathematical statistics analysis,it is concluded that the wind-proof seed has a thousand-grain weight;Through the viability experiment,it is found that the viability of the Guan windproof is better than the F_(1 )generation Hailar windproof and Hailar windproof when the dyeing concentration is 0.1%,0.5%,1.0%.According to the germination experiment,the total germination rate of the Guan windproofseeds is higher than that of the Hailaer windproof and the F_1 generation Hailar windproof.Conclusion Guan windproof seeds are more active in three seeds.
引文
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[2] 国家药典委员会.中华人民共和国药典[M].北京:化学工业出版社,2015.
[3] 戴静波,王丽丽,朱祥英.辽宁防风挥发油成分的气相色谱-质谱联用分析[J].时珍国医国药,2011,22(7):1611-1612.
[4] 杨景明,姜华,孟祥才.中药防风质量评价的现状与思考[J].中药材,2016,39(7):1678-1681.
[5] 刘双利,姜程曦,赵岩,等.防风化学成分及其药理作用研究进展[J].中草药,2017,48(10):2146-2152.
[6] 中国标准出版社第一编辑室.中国农业标准汇编-种子苗木卷[M].北京:中国标准出版社,2010.
[7] 陈瑛.实用中药种子技术手册[M].北京:人民卫生出版社,1999.
[8] 余振宙,陶鸿,占梦丹,等.四唑染色法测定茄子种子生活力[J].江苏农业科学,2016,45(5):206-209.
[9] 颜启传.种子检验的原理和技术[M].浙江:浙江大学出版社,2001.
[10] 王志威,魏升华,李俊等.头花蓼种子生活力TTC法最优测定条件研究[J].南方农业,2018,12(25):1-6.
[11] 张薇,耿雷跃,杨雅华,等.水稻种子活力的快速测定方法[J].现代农业科技,2015,44(8):29-30.
[12] 应叶青,申亚梅,李浪.3种阔叶树种子生活力四唑法测定研究[J].种子,2005,24(1):32-35.
[13] 席沁,赵廷宁,张成梁.若干种子处理方法对偃松种子生活力的影响[J].植物科学学报,2014,32(4):427-433.
[14] 郭巧生.药用植物栽培学[M].北京:高等教育出版社,2009.
[15] 马琳,李珊珊,许世泉,等.东北铁线莲多糖提取及含量测定[J].特产研究,2014,53(4):60-62.
[16] 周艳玲,赵敏,赵雨森.防风种子的休眠机制[J].东北林业大学学报,2009,37(3):16-17.