HS-SPME-GC-MS分析桑不同药用部位的挥发性成分
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摘要
目的:对桑不同部位的挥发性成分进行分析比较。方法:采用顶空固相微萃取法(HS-SPME)和气质联用法(GC-MS)进行提取和分析比较。结果:从桑叶中分离出23个峰,鉴定出19个成分,占总挥发性成分的87.11%;从桑枝中分离出15个峰,鉴定出13个成分,占总挥发性成分的83.57%;从桑白皮中分离出21个峰,鉴定出16个成分,占总挥发性成分的90.37%;从桑椹中分离出13个峰,鉴定出10个成分,占总挥发性成分的82.65%。四者具有己醛、1-辛烯-3-醇、甲基庚烯酮、壬醛4种共有挥发性成分。结论:研究表明桑叶、桑枝、桑白皮和桑椹的挥发性成分及其含量有一定差异,四者的主要药理作用有所不同,故桑的不同药用部位在临床应用上不能相互替代,同时为进一步研究桑资源提供参考。
Objective:To analyze the volatile components in different parts of Morus alba L..Methods:HS-SPME and(GC-MS)was used to extract and analyze the volatile constituents in different parts of M.alba.Results:23 peaks were detected from Mori folium,19 components were identified,accounting for 87.11% of volatile components.15 peaks were detected from Mori Ramulus,13 components were identified,accounting for 83.57% of volatile components.21 peaks were detected from Mori Cortex,16 components were identified,accounting for 90.37% of volatile components.13 peaks were detected from Mori Fructus,10 components were identified,accounting for 82.65% of volatile components.Mori Folium,Mori Ramulus,Mori Cortex and Mori Fructus have 4 common volatile components of caproaldehyde,1-octen-3-ol,6-Methyl-5-hepten-2-one and 1-nonanal.Conclusion:Mori Folium,Mori Ramulus,Mori Cortex and Mori Fructus are different in volatile components and contents,and their main pharmacological effects are different,so the different medicinal parts of M.alba cannot be used as substitutes in clinical application.At the same time,the study provides a reference for further study of mulberry resources.
Objective:To analyze the volatile components in different parts of Morus alba L..Methods:HS-SPME and(GC-MS)was used to extract and analyze the volatile constituents in different parts of M.alba.Results:23 peaks were detected from Mori folium,19 components were identified,accounting for 87.11% of volatile components.15 peaks were detected from Mori Ramulus,13 components were identified,accounting for 83.57% of volatile components.21 peaks were detected from Mori Cortex,16 components were identified,accounting for 90.37% of volatile components.13 peaks were detected from Mori Fructus,10 components were identified,accounting for 82.65% of volatile components.Mori Folium,Mori Ramulus,Mori Cortex and Mori Fructus have 4 common volatile components of caproaldehyde,1-octen-3-ol,6-Methyl-5-hepten-2-one and 1-nonanal.Conclusion:Mori Folium,Mori Ramulus,Mori Cortex and Mori Fructus are different in volatile components and contents,and their main pharmacological effects are different,so the different medicinal parts of M.alba cannot be used as substitutes in clinical application.At the same time,the study provides a reference for further study of mulberry resources.
引文
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[14] 陈萍,王建刚.山苍子雌花挥发性成分的SPME-GC/MS分析[J].山东化工,2015,44(22):77-80.
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[18] 张荣发,杨宗发,江尚飞.薄荷油的药理毒理作用研究进展[J].中国药业,2012,21(19):1-3.
[19] 林月彬,王晖,梁庆,等.薄荷醇止痒作用的研究[J].中华中医药学刊,2009,27(7):1488-1490.
[20] 吴怀恩,李耀华,韦志英,等.广西五月艾、细叶艾与艾叶挥发油的比较研究[J].中国医药导报,2008,5(35):23-26.
[21] 蔡进章,林崇良,周子晔,等.山橿根、茎、叶挥发油化学成分的研究[J].中华中医药学刊,2011,29(8):1893-1895.
[2] 宿树兰,段金廒,欧阳臻,等.我国桑属(Morus L.)药用植物资源化学研究进展[J].中国现代中药,2012,14(7):1-6.
[3] 国家药典委员会.中华人民共和国药典:一部[M].北京:中国医药科技出版社,2015:297-300.
[4] 李季芳,蒋卓勤,程卫,等.桑树资源降血糖主要活性成分的研究进展[J].中国现代中药,2008,10(10):6-7,43.
[5] 李秀琴.柴胡质量评价方法与其相关成分的药动学研究[D].沈阳:沈阳药科大学,2006.
[6] 杨波,刘志刚,刘丹丹,等.鲍鱼菇菌丝体挥发油化学成分及抗菌活性分析[J].中国食用菌,2009,28(3):40-42.
[7] 余伯良,罗惠波,周健,等.山苍子油抗霉菌及抑制黄曲霉产毒的有效成分研究[J].四川轻化工学院学报,2002,15(1):32-36.
[8] 何跃君.竹叶挥发油化学成分及其生物活性研究[D].北京:中国林业科学研究院,2009.
[9] 盖苗苗,周春玲,曲宁,等.雪松的挥发性物质成分及抑菌效益研究[J].中国农学通报,2010,26(7):311-313.
[10] 房海灵,李维林,任冰如,等.薄荷属植物的化学成分及药理学研究进展[J].中国药业,2010,19(10):13-17.
[11] JAYAPRAKASHA G K,MURTHY K N,DEMARAIS R,et al.Inhibition of prostate cancer(LNCaP)cell proliferation by volatile components from Nagami Kumquats[J].Planta Med,2012,78(10):974-980.
[12] 陈昊.几种天然植物油脂肪酸组成及其保健功能[J].科技创新导报,2010(20):227-228.
[13] 蒋燕,蒲彪,王汉清,等.不同方法提取冷榨藤椒油中的油树脂及其成分分析[J].食品与机械,2016,32(1):130-134,157.
[14] 陈萍,王建刚.山苍子雌花挥发性成分的SPME-GC/MS分析[J].山东化工,2015,44(22):77-80.
[15] 徐建荣,张毅立,余玉梅,等.侧柏叶及其炮制品在卷烟滤嘴中的应用[J].湖北农业科学,2013,52(20):5009-5014.
[16] 熊颖,吴雪茹,涂兴明,等.樟脑的药学研究进展[J].检验医学与临床,2009,6(12):999-1001.
[17] 周慧秋,于滨,乔婉红,等.甲基丁香酚药理作用研究[J].中医药学报,2000(2):79-80.
[18] 张荣发,杨宗发,江尚飞.薄荷油的药理毒理作用研究进展[J].中国药业,2012,21(19):1-3.
[19] 林月彬,王晖,梁庆,等.薄荷醇止痒作用的研究[J].中华中医药学刊,2009,27(7):1488-1490.
[20] 吴怀恩,李耀华,韦志英,等.广西五月艾、细叶艾与艾叶挥发油的比较研究[J].中国医药导报,2008,5(35):23-26.
[21] 蔡进章,林崇良,周子晔,等.山橿根、茎、叶挥发油化学成分的研究[J].中华中医药学刊,2011,29(8):1893-1895.