Galectin-3基因调控Wnt/β-catenin信号通路对肺癌细胞凋亡的影响
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摘要
目的探讨半乳糖凝集素(Galectin)-3基因对肺癌细胞凋亡的影响及机制。方法将NC-siRNA、Galectin-3-siRNA1和Galectin-3-siRNA2转染人肺癌A549细胞,未转染任何的siRNA作为对照组,转染48 h后提取细胞中的蛋白,Western印迹检测各组细胞中Galectin-3蛋白表达;流式细胞仪检测细胞凋亡率;Western印迹检测酶切含半胱氨酸天冬氨酸蛋白水解酶(Cleaved caspase)3、β-链蛋白(catenin)、细胞周期蛋白(Cyclin)D1蛋白表达。结果 NC-siRNA组Galectin-3蛋白表达与对照组差异无统计学意义(P>0.05),Galectin-3-siRNA1、Galectin-3-siRNA2组Galectin-3蛋白表达均显著低于对照组(P<0.01),Galectin-3-siRNA1组的抑制效果更明显,选择作为后续研究;与对照组及NC-siRNA组比较,Galectin-3-siRNA组β-catenin、Cyclin D1蛋白表达显著降低,细胞凋亡率及Cleaved caspase3蛋白表达显著升高(P<0.01)。结论抑制Galectin-3在肺癌细胞中的表达可诱导细胞凋亡,其机制与抑制Wnt/β-catenin信号通路有关。
引文
1 Schapira MM,Aggarwal C,Akers S,et al.How patients view lung cancer screening.the role of uncertainty in medical decision making[J].Ann Am Thorac Soc,2016;13(11):1969-76.
2 Shimura T,Shibata M,Gonda K,et al.Circulating galectin-3 correlates with angiogenetic factors,indicators of nutritional condition and systemic inflammation in patients with thyroid cancer[J].Ann Cancer Res Ther,2016;24(1):35-40.
3 Brachs S,Winkel AF,Tang H,et al.Inhibition of citrate cotransporter Slc13a5/m INDY by RNAi improves hepatic insulin sensitivity and prevents diet-induced non-alcoholic fatty liver disease in mice[J].Mol Metab,2016;5(11):1072-82.
4 Park AM,Hagiwara S,Hsu DK,et al.Galectin-3 plays an important role in innate immunity to gastric infection by Helicobacter pylori[J].Infec Immun,2016;84(4):1184-93.
5 Shimura T,Kofunato Y,Okada R,et al.Intranuclear accumulation of galectin-3 is associated with a poor prognosis in patients with invasive intraductal papillary mucinous neoplasm[J].Ann Cancer Res Ther,2016;24(1):23-9.
6 Ilmer M,Mazurek N,Gilcrease MZ,et al.Low expression of galectin-3 is associated with poor survival in node-positive breast cancers and mesenchymal phenotype in breast cancer stem cells[J].Breast Cancer Res,2016;18(1):97.
7 申建刚,张定国,朱惠明.血管内皮生长因子-A siRNA对人肝癌Hep G2细胞凋亡的影响及机制[J].中国老年学杂志,2016;36(3):542-4.
8 Sohn D,Peters D,Piekorz RP,et al.miR-30e controls DNA damage-induced stress responses by modulating expression of the CDK inhibitor p21WAF1/CIP1 and caspase-3[J].Oncotarget,2016;7(13):15915.
9 Ye S,Zhang D,Cheng F,et al.Wnt/β-catenin and LIF-Stat3 signaling pathways converge on Sp5 to promote mouse embryonic stem cell self-renewal[J].J Cell Sci,2016;129(2):269-76.
10 Shi J,Jiang X,Yu Z,et al.ZNRF3 contributes to the growth of lung carcinoma via inhibiting Wnt/β-catenin pathway and is regulated by miR-93[J].Tumor Biol,2016;37(3):3051-7.
2 Shimura T,Shibata M,Gonda K,et al.Circulating galectin-3 correlates with angiogenetic factors,indicators of nutritional condition and systemic inflammation in patients with thyroid cancer[J].Ann Cancer Res Ther,2016;24(1):35-40.
3 Brachs S,Winkel AF,Tang H,et al.Inhibition of citrate cotransporter Slc13a5/m INDY by RNAi improves hepatic insulin sensitivity and prevents diet-induced non-alcoholic fatty liver disease in mice[J].Mol Metab,2016;5(11):1072-82.
4 Park AM,Hagiwara S,Hsu DK,et al.Galectin-3 plays an important role in innate immunity to gastric infection by Helicobacter pylori[J].Infec Immun,2016;84(4):1184-93.
5 Shimura T,Kofunato Y,Okada R,et al.Intranuclear accumulation of galectin-3 is associated with a poor prognosis in patients with invasive intraductal papillary mucinous neoplasm[J].Ann Cancer Res Ther,2016;24(1):23-9.
6 Ilmer M,Mazurek N,Gilcrease MZ,et al.Low expression of galectin-3 is associated with poor survival in node-positive breast cancers and mesenchymal phenotype in breast cancer stem cells[J].Breast Cancer Res,2016;18(1):97.
7 申建刚,张定国,朱惠明.血管内皮生长因子-A siRNA对人肝癌Hep G2细胞凋亡的影响及机制[J].中国老年学杂志,2016;36(3):542-4.
8 Sohn D,Peters D,Piekorz RP,et al.miR-30e controls DNA damage-induced stress responses by modulating expression of the CDK inhibitor p21WAF1/CIP1 and caspase-3[J].Oncotarget,2016;7(13):15915.
9 Ye S,Zhang D,Cheng F,et al.Wnt/β-catenin and LIF-Stat3 signaling pathways converge on Sp5 to promote mouse embryonic stem cell self-renewal[J].J Cell Sci,2016;129(2):269-76.
10 Shi J,Jiang X,Yu Z,et al.ZNRF3 contributes to the growth of lung carcinoma via inhibiting Wnt/β-catenin pathway and is regulated by miR-93[J].Tumor Biol,2016;37(3):3051-7.