正交优选长春花总黄酮提取工艺及抗氧化研究
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摘要
目的:优选长春花总黄酮的提取工艺及研究其抗氧化能力。方法:采用乙醇—水提取方法从长春花中提取总黄酮,在波长506nm处分别测定长春花总黄酮的提取率。采用L_9(3~3)正交试验优选长春花总黄酮的提取工艺,并利用普鲁士兰法测定其黄酮还原Fe~(3+)能力。结果:长春花总黄酮的最佳提取工艺为每次提取1h,乙醇体积分数50%,料液比1∶50(g/mL),在此条件下总黄酮的收率为6.17%。长春花总黄酮具有较强的还原能力,但还原Fe~(3+)能力低于2,6-二叔丁基对甲酚(BHT)。结论:试验结果表明,优选的提取工艺较好,可靠方便,其黄酮还原Fe~(3+)能力较强。
Objective :To optimize the extraction process of total flavonoids from Catharanthus Roseus and study its antioxidative capacity. Methods:Ethanol-water extraction was used to extract total flavonoids from periwinkle flowers. The extraction rate of total flavonoids from Catharanthus Roseus was determined at a wavelength of 506 nm.The extraction process of total flavonoids from Catharanthus Roseus was optimized by L_9(3~3) orthogonal test. The ability of flavonoids in reducing Fe~(3+) was determined by Prussian blue method. Results:The optimum extraction process was 1 h,the volume fraction of ethanol was 50%,and the ratio of solid to liquid was 1:50(g/mL). Under the above condition,the yield of total flavonoids was 6.17%.The total flavonoids of Catharanthus Roseus had a strong reducing ability,but the ability to reduce Fe~(3+) is lower than that of 2,6-Di-tert-butyl-p-cresol(BHT). Conclusions:The experimental results showed that the optimal extraction process is better,reliable and convenient,and a stronger ability to reduce Fe~(3+).
Objective :To optimize the extraction process of total flavonoids from Catharanthus Roseus and study its antioxidative capacity. Methods:Ethanol-water extraction was used to extract total flavonoids from periwinkle flowers. The extraction rate of total flavonoids from Catharanthus Roseus was determined at a wavelength of 506 nm.The extraction process of total flavonoids from Catharanthus Roseus was optimized by L_9(3~3) orthogonal test. The ability of flavonoids in reducing Fe~(3+) was determined by Prussian blue method. Results:The optimum extraction process was 1 h,the volume fraction of ethanol was 50%,and the ratio of solid to liquid was 1:50(g/mL). Under the above condition,the yield of total flavonoids was 6.17%.The total flavonoids of Catharanthus Roseus had a strong reducing ability,but the ability to reduce Fe~(3+) is lower than that of 2,6-Di-tert-butyl-p-cresol(BHT). Conclusions:The experimental results showed that the optimal extraction process is better,reliable and convenient,and a stronger ability to reduce Fe~(3+).
引文
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[12]葛伟,李晓东,曲佳林,等.超声预处理对酪蛋白美拉德反应产物抗氧化性和结构的影响[J].中国食品学报,2018,18(2):125-133.
[2]杨莹莹,张广晶,张舒媛,等.长春花药理作用研究进展[J].西部中医药,2014,27(10):170-172.
[3]Alex Van Moerkercke,Priscille Steensma,Ivo Gariboldi,et al.The basic helix-loop-helix Transcription factor BIS 2 is essential for monoterpenoid indole alkaloid production in the medicinal plant Catharanthus roseus[J].Plant J,2016,88(1):3-12.
[4]王华庆,姜文奇,石远凯,等.长春碱类药物治疗恶性淋巴瘤中国专家共识[J].中国肿瘤临床,2017,44(5):193-198.
[5]Mardani-Nejad Shahin,Khavari-Nejad Ramazan Ali,Saadatmand Sara,et al.Potent Antioxidant Properties of rolB-transformed Catharanthus roseus(L.)G.Don.[J].Iranian journal of pharmaceutical research:IJPR,2016,15(2):537-50.
[6]聂挺.量子化学计算研究多酚及多肽清除自由基的构效关系[D].南昌大学,2016:1-68.
[7]李鹏,马,吴浩宇,等.大豆异黄酮对阿特拉津损伤SH-SY5Y细胞的保护作用及其机制[J].癌变·畸变·突变,2018,30(1):25-30.
[8]张怀民,杨虹,郑海洲.天然黄酮类化合物防治心脑血管疾病的研究进展[J].中国新药与临床杂志,2016,35(10):704-708.
[9]靳萍奎,姜相明,顾平,等.小蔓长春花提取物对大鼠脑梗死的保护作用[J].江苏医药,2012,3(23):2780-2783.
[10]余丽丽,汪娇梅,黄锁义,等.广金钱草总黄酮含量测定及还原Fe3+的能力[J].中国野生植物资源,2014,33(5):26-28.
[11]齐梁煜,黄月维,张航,等.王不留行总黄酮提取工艺及抗氧化活性研究[J].中国药师,2016,19(12):2244-2246,2261.
[12]葛伟,李晓东,曲佳林,等.超声预处理对酪蛋白美拉德反应产物抗氧化性和结构的影响[J].中国食品学报,2018,18(2):125-133.