芝麻种质资源抗青枯病鉴定
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摘要
采用自然病圃法和伤根灌注法对29份芝麻种质资源进行了青枯病抗性鉴定。结果表明:不同品种(株系)间的抗性存在较大差异。在自然病圃鉴定中,中抗品种4份,中感品种(株系)7份,感病品种(株系)13份,高感品种(株系)5份。在灌根接种鉴定中,中抗品种4份,中感品种(株系)6份,感病品种(株系)12份,高感品种(株系)7份。在两种鉴定中,金黄麻、鄱阳黑芝麻、赣芝5号、豫芝11均表现为中抗。对两种方法的鉴定结果(病情指数)进行聚类分析,29份材料均可聚为5个组。
The resistances of 29 sesame(Sesamum indicum) germplasm resources to bacterial wilt(Ralstorinia solanacearum) were identified by using the spontaneous diseased-nursery infection method and the root-irrigating inoculation method.The results indicated that there were greater differences in bacterial-wilt-resistance among 29 sesame varieties(lines).In the identification by the spontaneous diseased-nursery infection method,there were 4 moderately-resistant varieties(lines),7 moderately-susceptible varieties(lines),13 susceptible varieties(lines),and 5 highly-susceptible varieties(lines).In the identification by the root-irrigating inoculation method,there were 4 moderately-resistant varieties(lines),6 moderately-susceptible varieties(lines),12 susceptible varieties(lines),and 7 highly-susceptible varieties(lines).In the identification by the above two methods,4 sesame varieties(lines) were found to be moderately resistant to R.solanacearum,namely Jinhuangma,Poyang black sesame,Ganzhi No.5 and Yuzhi 11.The clustering analysis of the identification results(disease index) by two methods showed 29 sesame varieties(lines) could be clustered into 5 groups.
The resistances of 29 sesame(Sesamum indicum) germplasm resources to bacterial wilt(Ralstorinia solanacearum) were identified by using the spontaneous diseased-nursery infection method and the root-irrigating inoculation method.The results indicated that there were greater differences in bacterial-wilt-resistance among 29 sesame varieties(lines).In the identification by the spontaneous diseased-nursery infection method,there were 4 moderately-resistant varieties(lines),7 moderately-susceptible varieties(lines),13 susceptible varieties(lines),and 5 highly-susceptible varieties(lines).In the identification by the root-irrigating inoculation method,there were 4 moderately-resistant varieties(lines),6 moderately-susceptible varieties(lines),12 susceptible varieties(lines),and 7 highly-susceptible varieties(lines).In the identification by the above two methods,4 sesame varieties(lines) were found to be moderately resistant to R.solanacearum,namely Jinhuangma,Poyang black sesame,Ganzhi No.5 and Yuzhi 11.The clustering analysis of the identification results(disease index) by two methods showed 29 sesame varieties(lines) could be clustered into 5 groups.
引文
[1]魏利斌,苗红梅,李春,等.芝麻SNP和In Del标记遗传多样性、群体结构及连锁不平衡分析[J].分子植物育种,2017,15(8):3070-3079.
[2]华菊玲,胡白石,李湘民,等.芝麻细菌性青枯病病原菌及其生化变种鉴定[J].植物保护学报,2012,39(2):39-44.
[3]Wicker E,Grassart L,Coranson-Beaudu R,et al.Ralstonia solanacearum strains from Martinique(French West Indies)exhibiting a new pathogenic potential[J].Applied and Environmental Microbiology,2007,71(21):6790-6801.
[4]华菊玲,刘光荣,黄劲松.连作对芝麻根际土壤微生物群落的影响[J].生态学报,2012,32(9):2937-2942.
[5]李信申,肖运萍,魏林根,等.芝麻青枯雷尔氏菌生化特性及致病力分化的研究[J].江西农业学报,2016,28(12):61-65.
[6]唐启义,冯明光.实用统计分析及其计算机处理平台[M].北京:中国农业出版社,1997.
[7]徐进,冯洁.植物青枯菌遗传多样性及致病基因组学研究进展[J].中国农业科学,2013,46(14):2902-2909.
[8]Bertolla F,Pepin R,Passelegue R,et al.Plant genome complexity may be a factor limiting in situ the transfer of transgenic plant genes to the phytopathogen Ralstonia solanacearum[J].Applied and Environmental Microbiology,2000,66(9):4161-4167.
[9]廖伯寿,许泽永,姜慧芳.植物细菌性青枯病抗性的分子标记研究与育种潜力[J].中国油料作物学报,2001,23(3):66-68.
[10]Ho G,Yang C.A single locus leads to resistance of Arabidopsis thaliana to bacterial wilt caused by Ralstonia solanacearum through a hypersensitive-like response[J].Phytopathology,1999,89(8):673-678.
[11]Deslandes L.Genetic characterization of RRS1,a recessive locus in Arabidopsis thaliana that confers resistance to the bacterial soliborne pathogen Ralstonia solanacearum[J].Mol Plaint-Microbe Interact,1998(11):659-667.
[12]刘波,林营志,朱育菁,等.青枯雷尔氏菌多态性研究[M].福州:福建科学技术出版社,2005:26-42.
[13]Danesh D,Young N D.Partial resistance locus for tomato bacterial wilt show differential race specificity[J].Tomato Genetics Cooperative Report,1996,44:12-13.
[14]Jack A M.The CORESTA collaborative study on bacterial wilt(Ralstonia solanacearum)2002 report[J].CORESTA Information Bulletin,2002(4):45-58.
[15]张振臣,袁清华,马柱文,等.烟草品种GDSY-1的青枯病抗性与遗传分析[J].中国烟草科学,2017,38(4):9-15.
[16]Allen C,Gay J,Lauteanon S B.A regulatory locus,heh SR,controls polygalacturonasw production and other virulence functions on Ralstonia solanacearum[J].MPMI,1996(9):826-835.
[17]李映,蔡兴奎,李林章,等.马铃薯本细胞杂种的青枯病抗性鉴定[J].中国马铃薯,2005,19(4):198-203.
[18]Nimmakayala P,Perumal R,Mulpuri S,et al.Sesamum[C]//Kole C.Wild crop relatives:genomic and breeding resources oilseeds.Berlin Heidelberg:Springer-Verlag,2011:261-273.
[19]Zhang H Y,Miao H M,Li C,et al.Ultra-dense SNP genetic map construction and identification of Si Dt gene controlling the determinate growth habit in Sesamum indicum L.[J].Sci Rep,2016(6):31556.
[20]Grimault V,Anais G,Prior P.Distribution of Pseudomonas solanacearum in the stem tissues of tomato plants with different levels of resistance to bacterial wilt[J].Plant Pathology,1994,43(4):663-668.
[21]刘波,林营志,朱育菁,等.青枯雷尔氏菌多态性研究[M].福州:福建科学技术出版社,2005:26-42.
[22]郭堂勋,莫贱友.番茄砧木品种材料抗青枯病接种鉴定试验[J].广西农业科学,2009,40(3):250-252.
[23]周桂元,梁炫强,李一聪,等.花生青枯病抗性鉴定及抗源分析[J].花生学报,2003,32(3):25-28.
[24]王晓丹,闵凡祥,郭梅,等.马铃薯青枯病菌生化型研究及菌株接种方法的比较[J].中国马铃薯,2010,24(1):38-40.
[25]许秀玉,张卫强,黄钰辉,等.木麻黄青枯病抗性鉴定方法比较及抗病种质筛选[J].华南农业大学学报,2017,38(4):87-94.
[2]华菊玲,胡白石,李湘民,等.芝麻细菌性青枯病病原菌及其生化变种鉴定[J].植物保护学报,2012,39(2):39-44.
[3]Wicker E,Grassart L,Coranson-Beaudu R,et al.Ralstonia solanacearum strains from Martinique(French West Indies)exhibiting a new pathogenic potential[J].Applied and Environmental Microbiology,2007,71(21):6790-6801.
[4]华菊玲,刘光荣,黄劲松.连作对芝麻根际土壤微生物群落的影响[J].生态学报,2012,32(9):2937-2942.
[5]李信申,肖运萍,魏林根,等.芝麻青枯雷尔氏菌生化特性及致病力分化的研究[J].江西农业学报,2016,28(12):61-65.
[6]唐启义,冯明光.实用统计分析及其计算机处理平台[M].北京:中国农业出版社,1997.
[7]徐进,冯洁.植物青枯菌遗传多样性及致病基因组学研究进展[J].中国农业科学,2013,46(14):2902-2909.
[8]Bertolla F,Pepin R,Passelegue R,et al.Plant genome complexity may be a factor limiting in situ the transfer of transgenic plant genes to the phytopathogen Ralstonia solanacearum[J].Applied and Environmental Microbiology,2000,66(9):4161-4167.
[9]廖伯寿,许泽永,姜慧芳.植物细菌性青枯病抗性的分子标记研究与育种潜力[J].中国油料作物学报,2001,23(3):66-68.
[10]Ho G,Yang C.A single locus leads to resistance of Arabidopsis thaliana to bacterial wilt caused by Ralstonia solanacearum through a hypersensitive-like response[J].Phytopathology,1999,89(8):673-678.
[11]Deslandes L.Genetic characterization of RRS1,a recessive locus in Arabidopsis thaliana that confers resistance to the bacterial soliborne pathogen Ralstonia solanacearum[J].Mol Plaint-Microbe Interact,1998(11):659-667.
[12]刘波,林营志,朱育菁,等.青枯雷尔氏菌多态性研究[M].福州:福建科学技术出版社,2005:26-42.
[13]Danesh D,Young N D.Partial resistance locus for tomato bacterial wilt show differential race specificity[J].Tomato Genetics Cooperative Report,1996,44:12-13.
[14]Jack A M.The CORESTA collaborative study on bacterial wilt(Ralstonia solanacearum)2002 report[J].CORESTA Information Bulletin,2002(4):45-58.
[15]张振臣,袁清华,马柱文,等.烟草品种GDSY-1的青枯病抗性与遗传分析[J].中国烟草科学,2017,38(4):9-15.
[16]Allen C,Gay J,Lauteanon S B.A regulatory locus,heh SR,controls polygalacturonasw production and other virulence functions on Ralstonia solanacearum[J].MPMI,1996(9):826-835.
[17]李映,蔡兴奎,李林章,等.马铃薯本细胞杂种的青枯病抗性鉴定[J].中国马铃薯,2005,19(4):198-203.
[18]Nimmakayala P,Perumal R,Mulpuri S,et al.Sesamum[C]//Kole C.Wild crop relatives:genomic and breeding resources oilseeds.Berlin Heidelberg:Springer-Verlag,2011:261-273.
[19]Zhang H Y,Miao H M,Li C,et al.Ultra-dense SNP genetic map construction and identification of Si Dt gene controlling the determinate growth habit in Sesamum indicum L.[J].Sci Rep,2016(6):31556.
[20]Grimault V,Anais G,Prior P.Distribution of Pseudomonas solanacearum in the stem tissues of tomato plants with different levels of resistance to bacterial wilt[J].Plant Pathology,1994,43(4):663-668.
[21]刘波,林营志,朱育菁,等.青枯雷尔氏菌多态性研究[M].福州:福建科学技术出版社,2005:26-42.
[22]郭堂勋,莫贱友.番茄砧木品种材料抗青枯病接种鉴定试验[J].广西农业科学,2009,40(3):250-252.
[23]周桂元,梁炫强,李一聪,等.花生青枯病抗性鉴定及抗源分析[J].花生学报,2003,32(3):25-28.
[24]王晓丹,闵凡祥,郭梅,等.马铃薯青枯病菌生化型研究及菌株接种方法的比较[J].中国马铃薯,2010,24(1):38-40.
[25]许秀玉,张卫强,黄钰辉,等.木麻黄青枯病抗性鉴定方法比较及抗病种质筛选[J].华南农业大学学报,2017,38(4):87-94.