美洲大蠊粉对腹泻小鼠结肠酶活性及其体内菌群增殖的影响
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摘要
为了明确美洲大蠊粉对腹泻小鼠结肠酶系活性及菌落数的影响,采用腹腔注射大肠杆菌建立小鼠腹泻模型,以饲料添加含量为8%(高剂量组)、5%(中剂量组)、2.5%(低剂量组)的美洲大蠊粉对其进行治疗,以紫外分光光度法检测小鼠结肠中SOD、POD、CAT、GSH、ACHE、ACP、LDH、AMS、LPS活性,以平板菌落计数法统计小鼠结肠的总菌和大肠杆菌数量。结果显示,腹泻引起小鼠结肠组织中相关酶系活性下降。饲喂含不同剂量的美洲大蠊粉的饲料后,高剂量治疗组小鼠结肠的SOD、高剂量和中剂量治疗组小鼠的POD、3种剂量治疗组小鼠的CAT均显著高于模型组(p<0.05);各剂量治疗组小鼠的GSH、ACHE显著高于模型组(p<0.05);高剂量和中剂量组小鼠的ACP显著高于模型组(p<0.05);各剂量治疗组的LPS显著高于模型组(p<0.05);高剂量和中剂量治疗组的AMS、LDH显著高于模型组(p<0.05);各剂量组小鼠的大肠杆菌数、总菌数显著低于模型组(p<0.05),其中高剂量治疗组小鼠的大肠杆菌数量低于空白组。上述结果表明,美洲大蠊粉能够增强小鼠抗腹泻能力。且以高、中剂量治疗腹泻小鼠的效果最好。
In order to clarify the effect of Periplaneta americana powder on the activity of enzyme system and colony number in diarrhea mouse of colon. The diarrhea mouse model was established by intraperitoneal injection with Escherichia coli, and the diarrhea mice were treated by the P.americana powder with 8%(high dose group), 5%(medium dose group), and 2.5%(low dose group) supplemented in daily feed. The activity of SOD, POD, CAT, GSH, ACHE, ACP, LDH, AMS and LPS in the mouse colon were detected by ultraviolet spectrophotometry, and total number of bacteria and E.coli in mouse colon were counted by plate colony counting method. The results showed that diarrhea caused a decrease of the activity of related enzyme system in the colon tissue of mouse. After the mice were treated with P.americana powder, the SOD of the mice in the high dose group, the POD in the high and middle dose group, as well as the CAT in all of three dose groups were significantly higher than that in the model group; the GSH and ACHE of mouse in each dose treatment group were significantly higher than that of the model group, while the ACP in the high and middle dose group was significantly higher than that of the model group; the LPS in each dose treatment group was significantly higher than that in model group, while the AMS, and LPS of the high and medium dose treatment group were significantly higher than that in the model group; the number of E.coli and total bacteria in each dose group were significantly lower than that in the model in group, among which the number of E.coli in the high-dose treatment group was even lower than that in blank control group. In conclusion, the P.americana powder could enhance the ability of anti-diarrhea in mice.The effect of high and medium dose treatment was the best.
In order to clarify the effect of Periplaneta americana powder on the activity of enzyme system and colony number in diarrhea mouse of colon. The diarrhea mouse model was established by intraperitoneal injection with Escherichia coli, and the diarrhea mice were treated by the P.americana powder with 8%(high dose group), 5%(medium dose group), and 2.5%(low dose group) supplemented in daily feed. The activity of SOD, POD, CAT, GSH, ACHE, ACP, LDH, AMS and LPS in the mouse colon were detected by ultraviolet spectrophotometry, and total number of bacteria and E.coli in mouse colon were counted by plate colony counting method. The results showed that diarrhea caused a decrease of the activity of related enzyme system in the colon tissue of mouse. After the mice were treated with P.americana powder, the SOD of the mice in the high dose group, the POD in the high and middle dose group, as well as the CAT in all of three dose groups were significantly higher than that in the model group; the GSH and ACHE of mouse in each dose treatment group were significantly higher than that of the model group, while the ACP in the high and middle dose group was significantly higher than that of the model group; the LPS in each dose treatment group was significantly higher than that in model group, while the AMS, and LPS of the high and medium dose treatment group were significantly higher than that in the model group; the number of E.coli and total bacteria in each dose group were significantly lower than that in the model in group, among which the number of E.coli in the high-dose treatment group was even lower than that in blank control group. In conclusion, the P.americana powder could enhance the ability of anti-diarrhea in mice.The effect of high and medium dose treatment was the best.
引文
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[4]张细元,高源,梅红,彭罕鸣.迁延性腹泻病患儿内镜特点及血清、结肠灌洗液中一氧化氮、丙二醛、超氧化物歧化酶水平变化的意义[J].实用儿科临床杂志,2012,27(19):1499-1501.
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[6]张松乐,陈梅玲,张朝隆,等.用平板计数和聚合酶链反应法检测湖水中大肠杆菌基因工程菌的稳定性[J].卫生研究,1998, 27(1):154-156.
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[10] David T, Bolick, James K, et al. Enteroaggregative Escherichia coli strain in a novel weaned mouse model:Exacerbation by malnutrition, biofilm as a virulence factor and treatment by nitazoxanide[J]. J Med Microbiol, 2013, 62(6):896-905.
[11] Bolick D T, Kolling G L, Moore J H, et al. Zinc deficiency alters host response and pathogen virulence in a mouse model of enteroaggregative escherichia coli induced diarrhea[J]. Gut Mi-crobes, 2014, 5(5):618-627.
[12]何小姣,奚再兴.美洲大蠊提取液对溃疡性结肠炎大鼠结肠黏膜IGF-1、TGF-(3表达的影响[J].现代医药卫生,2017,33(3):376-378.
[13] Zhang Hong-wei,Wei Li-you, Zhao Gang, et al. Periplaneta americana extract used in patients with systemic inflammatory response syndrome[J]. World J Emerg Med, 2016, 7(1):50-54.
[14] Cuzzcrea S, Riley D P, Caputi A P, et al. Antioxidant therapy a new phamacological approach in shock inflammation and ischemia reperfusion injury[J]. Phamacol Rev, 2001,53(1):135-159.
[15]王奎,冯颖,何钊,等.美洲大蠊中两个有抑菌活性的单甘酯的研究[J].时珍国医国药,2013, 24(9):2102-2103.
[16]肖小芹.美洲大蠊生物学特性及药用价值研究[D].长沙:中南大学,2008.
[2]蓝江林,周先治,卓侃,等.美洲大蠊(Periplaneta americana L.)抗菌肽杀菌作用初步观察[J].福建农林大学学报(自然科学版),2004, 33(2):166-169.
[3]周琼,吴珍泉,李忠荣,等.美洲大蠊粉对腹泻小鼠肠道修复的影响[J].福建农业学报,2008,23(2):125-131.
[4]张细元,高源,梅红,彭罕鸣.迁延性腹泻病患儿内镜特点及血清、结肠灌洗液中一氧化氮、丙二醛、超氧化物歧化酶水平变化的意义[J].实用儿科临床杂志,2012,27(19):1499-1501.
[5]马梦琳,赵德明.仔猪断奶期间腹泻及肠道酶肠道菌群的变化[J].中国兽医杂志,2000,26(10):39-40.
[6]张松乐,陈梅玲,张朝隆,等.用平板计数和聚合酶链反应法检测湖水中大肠杆菌基因工程菌的稳定性[J].卫生研究,1998, 27(1):154-156.
[7] Zerpa R, Huicho L. Childhood cryptosporidial diarrhea associat-ed with identification of Cryptosporidium SP in the cockroach periplaneta americana[J]. Pediatric Inf Dis J,1994,13(6):546-548.
[8] Agbodaze D, Owusu S B. Cockroaches(Periplaneta americana)as carriers of agents of bacterlal diarrhea in accra, Ghana[J].Cent Afr J Med, 1989, 35(9):484-486.
[9] Luiz W B, Rodrigues J F, Crabb J H, et al. Maternal vaccination with a fimbrial tip adhesin and passive protection of neonatal mice against lethal human enterotoxigenic Escherichia coli challenge[J]. Infect Immun, 2015, 83(12):4555-4564.
[10] David T, Bolick, James K, et al. Enteroaggregative Escherichia coli strain in a novel weaned mouse model:Exacerbation by malnutrition, biofilm as a virulence factor and treatment by nitazoxanide[J]. J Med Microbiol, 2013, 62(6):896-905.
[11] Bolick D T, Kolling G L, Moore J H, et al. Zinc deficiency alters host response and pathogen virulence in a mouse model of enteroaggregative escherichia coli induced diarrhea[J]. Gut Mi-crobes, 2014, 5(5):618-627.
[12]何小姣,奚再兴.美洲大蠊提取液对溃疡性结肠炎大鼠结肠黏膜IGF-1、TGF-(3表达的影响[J].现代医药卫生,2017,33(3):376-378.
[13] Zhang Hong-wei,Wei Li-you, Zhao Gang, et al. Periplaneta americana extract used in patients with systemic inflammatory response syndrome[J]. World J Emerg Med, 2016, 7(1):50-54.
[14] Cuzzcrea S, Riley D P, Caputi A P, et al. Antioxidant therapy a new phamacological approach in shock inflammation and ischemia reperfusion injury[J]. Phamacol Rev, 2001,53(1):135-159.
[15]王奎,冯颖,何钊,等.美洲大蠊中两个有抑菌活性的单甘酯的研究[J].时珍国医国药,2013, 24(9):2102-2103.
[16]肖小芹.美洲大蠊生物学特性及药用价值研究[D].长沙:中南大学,2008.