肉鸡消化道微生物区系分布特征及其关键影响因素
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摘要
肉鸡的消化道中寄居着数量庞大的微生物群落并参与机体的养分代谢和稳态调节。现代微生物分析技术的成功应用使人们对家禽消化道菌群的类别、数量、分布及功能均有了更加深入的认识。本文总结了微生物在肉鸡消化道的分布情况和定植规律,并重点阐述了遗传、日龄、饲养环境和饲粮等关键因素对肉鸡消化道微生物区系的影响及其作用效果,以期为开展肉鸡肠道健康调控技术提供理论基础。
There are a large number of microbial communities in broilers' digestive tract which participate in nutrient metabolism and homeostasis of the host. The successful application of modern technologies in sequencing and analysis of microbial communities has brought us a deeper understanding of the species,amount,distribution and functions of poultry gastrointestinal microflora. This reviewsummarized the distribution and colonization rules of gastrointestinal microflora of broilers and its key influencing factors,including heredity,days of age,environment and diets,that affected digestive tract microbes of broilers,with the aim to provide a theoretical basis for exploring scientific strategies to manage the intestinal health of broilers.[Chinese Journal of Animal Nutrition,2019,31( 6) : 2515-2524]
There are a large number of microbial communities in broilers' digestive tract which participate in nutrient metabolism and homeostasis of the host. The successful application of modern technologies in sequencing and analysis of microbial communities has brought us a deeper understanding of the species,amount,distribution and functions of poultry gastrointestinal microflora. This reviewsummarized the distribution and colonization rules of gastrointestinal microflora of broilers and its key influencing factors,including heredity,days of age,environment and diets,that affected digestive tract microbes of broilers,with the aim to provide a theoretical basis for exploring scientific strategies to manage the intestinal health of broilers.[Chinese Journal of Animal Nutrition,2019,31( 6) : 2515-2524]
引文
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[39]SCOTT K P,GRATZ S W,SHERIDAN P O,et al.The influence of diet on the gut microbiota[J].Pharmacological Research,2013,69(1):52-60.
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[45]TANCHAROENRAT P,RAVINDRAN V,MOLANA L,et al.Influence of fat source and xylanase supplementation on performance,utilisation of energy and fat,and caecal microbiota counts in broiler starters fed w heat-based diets[J].The Journal of Poultry Science,2014,51(2):172-179.
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[47]DANICKE S,VAHJEN W,SIMON O,et al.Effects of dietary fat type and xylanase supplementation to ryebased broiler diets on selected bacterial groups adhering to the intestinal epithelium.on transit time of feed,and on nutrient digestibility[J].Poultry Science,1999,78(9):1292-1299.
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[2]WEI S,MORRISON M,YU Z.Bacterial census of poultry intestinal microbiome[J].Poultry Science,2013,92(3):671-683.
[3]XIAO Y,XIANG Y,ZHOU W,et al.Microbial community mapping in intestinal tract of broiler chicken[J].Poultry Science,2016,96(5):1387-1393.
[4]CHOI J H,KIM G B,CHA C J.Spatial heterogeneity and stability of bacterial community in the gastrointestinal tracts of broiler chickens[J].Poultry Science,2014,93(8):1942-1950.
[5]DUGGETT N.High-throughput sequencing of the chicken-gut microbiome[D].Ph.D.Thesis.Birmingham:University of Birmingham,2016.
[6]REHMAN H U,VAHJEN W,AWAD W A,et al.Indigenous bacteria and bacterial metabolic products in the gastrointestinal tract of broiler chickens[J].Archives of Animal Nutrition,2007,61(5):319-335.
[7]HAN G G,KIM E B,LEE J,et al.Relationship betw een the microbiota in different sections of the gastrointestinal tract,and the body w eight of broiler chickens[J].Springerplus,2016,5:911.
[8]OAKLEY B B,LILLEHOJ H S,KOGUT M H,et al.The chicken gastrointestinal microbiome[J].FEM SM icrobiology Letters,2014,360(2):100-112.
[9]STANLEY D,HUGHES R J,MOORE R J.Microbiota of the chicken gastrointestinal tract:influence on health,productivity and disease[J].Applied M icrobiology and Biotechnology,2014,98(10):4301-4310.
[10]SEKELJA M,RUD I,KNUTSEN S H,et al.Abrupt temporal fluctuations in the chicken fecal microbiota are explained by its gastrointestinal origin[J].Applied and Environmental M icrobiology,2012,78(8):2941-2948.
[11]VIDEVALL E,STRANDH M,ENGELBRECHT A,et al.M easuring the gut microbiome in birds:comparison of faecal and cloacal sampling[J].M olecular Ecology Resources,2018,18(3):424-434.
[12]DONALDSON E E,STANLEY D,HUGHES R J,et al.The time-course of broiler intestinal microbiota development after administration of cecal contents to incubating eggs[J].PeerJ,2017,5:e3587.
[13]周小娟,张日俊,郭宝林.不同品种、日龄和饲养方式对肉鸡肠道菌群的影响[J].中国微生态学杂志,2012,24(7):577-581,587.
[14]HOU Q C,KWOK L Y,ZHENG Y,et al.Differential fecal microbiota are retained in broiler chicken lines divergently selected for fatness traits[J].Scientific Reports,2016,6:37376.
[15]MENG H,ZHANG Y,ZHAO L L,et al.Body weight selection affects quantitative genetic correlated responses in gut microbiota[J].PLoS One,2014,9(3):e89862.
[16]MORENO-NAVARRETE J M,JOVE M,ORTEGAF,et al.M etabolomics uncovers the role of adipose tissue PDXK in adipogenesis and systemic insulin sensitivity[J].Diabetologia,2016,59(4):822-832.
[17]NEIS E P J G,DEJONG C H C,RENSEN S S.The role of microbial amino acid metabolism in host metabolism[J].Nutrients,2015,7(4):2930-2946.
[18]ZHAO L L,WANG G,SIEGEL P,et al.Quantitative genetic background of the host influences gut microbiomes in chickens[J].Scientific Reports,2013,3:1163.
[19]MIGNON-GRASTEAU S,NARCY A,RIDEAU N,et al.Impact of selection for digestive efficiency on microbiota composition in the chicken[J].PLoS One,2015,10(8):e0135488.
[20]KOHL K D.Diversity and function of the avian gut microbiota[J].Journal of Comparative Physiology B,2012,182(5):591-602.
[21]PEDROSO A A,MENTEN JFM,LAMBAIS M R.The structure of bacterial community in the intestines of new ly hatched chicks[J].The Journal of Applied Poultry Research,2005,14(2):232-237.
[22]DING J M,DAI R H,YANG L Y,et al.Inheritance and establishment of gut microbiota in chickens[J].Frontiers in M icrobiology,2017,8:1967.
[23]RANJITKAR S,LAWLEY B,TANNOCK G,et al.Bacterial successions in the gastrointestinal tract of broilers[J].Applied and Environmental M icrobiology,2016,82(8):2399-2410.
[24]CISEK A A,BINEK M.Chicken intestinal microbiota function w ith a special emphasis on the role of probiotic bacteria[J].Polish Journal of Veterinary Sciences,2014,17(2):385-394.
[25]SHAUFI M A M,SIEO C C,CHONG C W,et al.Deciphering chicken gut microbial dynamics based on high-throughput 16S rRNA metagenomics analyses[J].Gut Pathogens,2015,7:4.
[26]PEDROSO A A,BATAL A B,LEE M D.Effect of in ovo administration of an adult-derived microbiota on establishment of the intestinal microbiome in chickens[J].American Journal of Veterinary Research,2016,77(5):514-526.
[27]VAN VEELEN H P J,SALLES J F,TIELEMAN B I.M icrobiome assembly of avian eggshells and their potential as transgenerational carriers of maternal microbiota[J].The ISM E Journal,2018,12(5):1375-1388.
[28]STANLEY D,GEIER M S,HUGHES R J,et al.Highly variable microbiota development in the chicken gastrointestinal tract[J].PLoS One,2013,8(12):e84290.
[29]CERMK L,SK RIVANOVE.Influence of pasture rearing on the cecal bacterial microbiota in broiler chickens[J].Scientia Agriculturae Bohemica,2016,47(3):124-128.
[30]WANG L L,LILBURN M,YU Z T.Intestinal microbiota of broiler chickens as affected by litter management regimens[J].Frontiers in M icrobiology,2016,7:593.
[31]KASSEM I I,SANAD Y,GANGAIAH D,et al.Use of bioluminescence imaging to monitor Campylobacter survival in chicken litter[J].Journal of Applied M icrobiology,2010,109(6):1988-1997.
[32]OAKLEY B B,VASCONCELOS E J R,DINIZ P P VP,et al.The cecal microbiome of commercial broiler chickens varies significantly by season[J].Poultry Science,2018,97(10):3635-3644.
[33]WANG X J,FENG J H,ZHANG M H,et al.Effects of high ambient temperature on the community structure and composition of ileal microbiome of broilers[J].Poultry Science,2018,97(6):2153-2158.
[34]彭骞骞,王雪敏,张敏红,等.持续偏热环境对肉鸡盲肠菌群多样性的影响[J].中国农业科学,2016,49(1):186-194.
[35]GUARDIA S,KONSAK B,COMBES S,et al.Effects of stocking density on the grow th performance and digestive microbiota of broiler chickens[J].Poultry Science,2011,90(9):1878-1889.
[36]常双双,柳青秀,张敏红,等.饲养密度对肉鸡盲肠菌群多样性、挥发性脂肪酸和血清脑肠肽的影响[J].动物营养学报,2018,30(3):938-946.
[37]常双双,石玉祥,厉秀梅,等.持续偏热环境对肉鸡血清和下丘脑脑肠肽含量、盲肠挥发性脂肪酸含量和菌群多样性的影响[J].动物营养学报,2018,30(2):560-569.
[38]LUDVIGSEN J,SVIHUS B,RUDI K.Rearing room affects the non-dominant chicken cecum microbiota,w hile diet affects the dominant microbiota[J].Frontiers in Veterinary Science,2016,3:16.
[39]SCOTT K P,GRATZ S W,SHERIDAN P O,et al.The influence of diet on the gut microbiota[J].Pharmacological Research,2013,69(1):52-60.
[40]WALUGEMBE M,HSIEH J C F,KOSZEWSKI N J,et al.Effects of dietary fiber on cecal short-chain fatty acid and cecal microbiota of broiler and laying-hen chicks[J].Poultry Science,2015,94(10):2351-2359.
[41]STEF L,DUMITRESCU G,DRINCEANU D,et al.Impact of w heat non-starch polysaccharides on the intestinal w all and microflora in broilers[J].Scientific Papers:Animal Science and Biotechnologies,2010,43(1):111-116.
[42]STEF L,DRINCEANU D,CORCIONIVOSCHI N,et al.The effect of dietary non-starch polysaccharides on the intestinal viscosity and on the cecal microflora of broiler fed w ith various protein sources[J].Archiva Zootechnica,2009,12(3):22-29.
[43]SAKI A A,MATIN H R H,TABATABAI M M,et al.M icroflora population,intestinal condition and performance of broilers in response to various rates of pectin and cellulose in the diet[J].Archiv fur Geflugelkunde,2010,74(3):183-188.
[44]VAN DER HOEVEN-HANGOOR E,VAN DERVOSSEN J M B M,SCHUREN F H J,et al.Ileal microbiota composition of broilers fed various commercial diet compositions[J].Poultry Science,2013,92(10):2713-2723.
[45]TANCHAROENRAT P,RAVINDRAN V,MOLANA L,et al.Influence of fat source and xylanase supplementation on performance,utilisation of energy and fat,and caecal microbiota counts in broiler starters fed w heat-based diets[J].The Journal of Poultry Science,2014,51(2):172-179.
[46]KNARREBORG A,SIMON M A,ENGBERG R M,et al.Effects of dietary fat source and subtherapeutic levels of antibiotic on the bacterial community in the ileum of broiler chickens at various ages[J].Applied and Environmental M icrobiology,2002,68(12):5918-5924.
[47]DANICKE S,VAHJEN W,SIMON O,et al.Effects of dietary fat type and xylanase supplementation to ryebased broiler diets on selected bacterial groups adhering to the intestinal epithelium.on transit time of feed,and on nutrient digestibility[J].Poultry Science,1999,78(9):1292-1299.
[48]胡虹,范国歌,张秀江,等.脂肪酶对肉鸡生产性能和肠道菌群的影响研究[J].河南科学,2013,31(9):1380-1382.
[49]QAISRANI S N,VAN KRIMPEN M M,KWAKKELR P,et al.Dietary factors affecting hindgut protein fermentation in broilers:a review[J].World’s Poultry Science Journal,2015,71(1):139-160.
[50]APAJALAHTI J,VIENOLA K.Interaction between chicken intestinal microbiota and protein digestion[J].Animal Feed Science and Technology,2016,221:323-330.
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