摘要
以苯并(a)芘(BaP)为目标污染物,探讨了3株细菌(BZSY-1、BZSY-2为芽孢杆菌; BZSY-3为动胶杆菌)在不同条件下对BaP细菌降解的酶蛋白应答以及降解率与酶活性的关系。结果表明:不同浓度BaP降解过程中,3株细菌的邻苯二酚2,3-双加氧酶(C23O)酶活性均较高,多酚氧化酶(PPO)酶活性均较低,而过氧化氢酶(CAT)酶活性与BaP的浓度无关;加入共代谢底物琥珀酸钠后,3株细菌的邻苯二酚1,2-双加氧酶(C12O)、C23O酶活性明显提高,CAT酶活性无明显变化,而PPO酶活性通过诱导依然很低;与对照相比,3株菌的C12O、C23O和PPO在TW80≤1000μg·m L~(-1)时酶活性不受影响,而当TW80>2000μg·m L~(-1)时,3株细菌的这3种酶均受到不同程度的抑制;与细菌BZSY-1相比,BZSY-2和BZSY-3对BaP的降解率较高,对应的几种酶活性(尤其是C12O、C23O)也比较高,进一步支持BaP的微生物降解是酶促反应,这3株细菌对BaP降解的关键酶是C12O和C23O。
The protein response of bacterial enzymes involved in degradation of benzo( a) pyrene( BaP) by three bacteria( BZSY-1,BZSY-2 for Bacillus,BZSY-3 for Zooloea sp.) were examined under different conditions. The relationships between the degradation rate and enzyme activity were analyzed. Four enzymes( C12O,C23O,CAT and PPO) were detected upon using different concentrations of BaP. Among the four enzymes,the activity of C23O was the highest and that of PPO was the lowest. The activity of CAT was not correlated with the concentration of BaP.After adding sodium succinate( co-metabolites),the activities of C12O and C23O were enhanced,whereas that of CAT was not changed,and the activity of PPO was still lower. Compared with the control,the activities of C12O,C23O and PPO were not affected when the concentration of TW80 was less than or equal to 1000 μg·mL~(-1). When the concentration of TW80 was greater than 2000 μg · m L~(-1),the activities of these three enzymes were inhibited by various degrees.Compared with BZSY-1,both BZSY-2 and BZSY-3 had higher BaP degradation rates and higher activities of the corresponding enzymes( especially C12O and C23O),which further supported the notion that the microbial degradation of BaP is an enzymatic reaction. C12O and C23O are the two key BaP-degrading enzymes in the three bacteria.
引文
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