湖南水稻主推品种对旱稻孢囊线虫的抗性及评价方法
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摘要
【目的】旱稻孢囊线虫是一种水稻上重要的病原线虫。筛选抗虫种质资源对抗病品种的选育至关重要。【方法】通过室内二龄幼虫接种和田间自然病圃鉴定,以单株平均孢囊数、相对抗病指数和繁殖系数法评价了湖南省51个主推的水稻品种对旱稻孢囊线虫的抗性。【结果】在供试水稻品种中未发现有免疫和高抗品种。室内接种条件下,在24个中稻品种中,依据单株平均孢囊数评价,仅广两优2010表现为抗;依据相对抗病指数评价,广两优2010、准两优527、广两优1128 3个品种为抗;在27个晚稻品种中,依据单株平均孢囊数评价,盛泰优9712、准两优608、农香优204、岳优9264、岳优3700、中浙优1号、岳优27、湘晚糯1号、贺优50、Y两优9918共10个品种为抗;而依据相对抗病指数评价,湘晚糯1号、贺优50、Y两优9918表现为中感。在田间自然病圃条件下,依据繁殖系数Pf/Pi评价,筛选出9个晚稻抗性品种,鉴定结果与室内接种相对抗病指数法基本一致。【结论】室内接种相对抗病指数法可以作为评价水稻品种对旱稻孢囊线虫抗性的有效方法。
【Objective】Heterodera elachista is a new pathogenic nematode of rice. In order to screen the resistant rice cultivars to H. elachista, 【Method】 51 rice varieties from Hunan Province were evaluated by the second-stage juvenile inoculation test in greenhouse and field with average number of cysts per plant, relative resistance index(RRI) and Pf/Pi ratio as indicators. 【Result】No rice cultivar was immune or highly resistant to H. elachista. Among the 24 middle-season rice cultivars, only Guangliangyou 2010 showed resistance to H. elachista based on average number of cysts per plant, and Guangliangyou 2010, Zhunliangyou 527 and Guangliangyou 1128 showed resistance to H. elachista based on RRI evaluation in greenhouse. Among the 27 late rice varieties, Shengtaiyou 9712, Zhunliangyou 608, Nongxiangyou 204, Yueyou 9264, Yueyou 3700, Zhongzheyou 1, Yueyou 27, Xiangwannuo 1, Heyou 50 and Y Liangyou 9918, were resistant to H. elachista based on average number of cyst per plant. However, Xiangwannuo 1, Heyou 50 and Y liangyou 9918 were moderately susceptible based on RRI evaluation. Using Pf/Pi ratio as the evaluation index, nine late resistant varieties screened from greenhouse based on RRI evaluation were basically consistent with field resistance. 【Conclusion】RRI is an efficient index for evaluating the resistance of rice cultivars to rice cyst nematode.
【Objective】Heterodera elachista is a new pathogenic nematode of rice. In order to screen the resistant rice cultivars to H. elachista, 【Method】 51 rice varieties from Hunan Province were evaluated by the second-stage juvenile inoculation test in greenhouse and field with average number of cysts per plant, relative resistance index(RRI) and Pf/Pi ratio as indicators. 【Result】No rice cultivar was immune or highly resistant to H. elachista. Among the 24 middle-season rice cultivars, only Guangliangyou 2010 showed resistance to H. elachista based on average number of cysts per plant, and Guangliangyou 2010, Zhunliangyou 527 and Guangliangyou 1128 showed resistance to H. elachista based on RRI evaluation in greenhouse. Among the 27 late rice varieties, Shengtaiyou 9712, Zhunliangyou 608, Nongxiangyou 204, Yueyou 9264, Yueyou 3700, Zhongzheyou 1, Yueyou 27, Xiangwannuo 1, Heyou 50 and Y Liangyou 9918, were resistant to H. elachista based on average number of cyst per plant. However, Xiangwannuo 1, Heyou 50 and Y liangyou 9918 were moderately susceptible based on RRI evaluation. Using Pf/Pi ratio as the evaluation index, nine late resistant varieties screened from greenhouse based on RRI evaluation were basically consistent with field resistance. 【Conclusion】RRI is an efficient index for evaluating the resistance of rice cultivars to rice cyst nematode.
引文
[1]Ohshima Y.Heterodera elachista n.sp.an upland rice cyst nematode from Japan.Nematol Res,1974,4:51-56.
[2]Shimizu K.Influence of the upland rice cyst nematode,Heterodera elachista on the yield of the upland-cultured paddy rice.Jpn J Nematol,1976,6:1-6.
[3]Ding Z,Namphueng J,He X F,Peng D L,Huang W K.First report of the cyst nematode(Heterodera elachista)on rice in Hunan Province,China.Plant Dis,2011,96(1):151.
[4]Luca F D,Vovlas N,Lucarelli G,et al.Heterodera elachista the Japanese cyst nematode parasitizing corn in Northern Italy:Integrative diagnosis and bionomics.Eur J Plant Pathol,2013,136(4):857-872.
[5]王水南,彭德良,黄文坤,丁中.旱稻孢囊线虫的快速分子检测.湖南农业大学学报:自然科学版,2014,40(2):178-182.Wang S N,Peng D L,Huang W K,Ding Z.Rapid molecular diagnosis of rice cyst nematode(Heterodera elachista).J Hunan Agric Univ:Nat Sci,2014,40(2):178-182.(in Chinese with English abstract)
[6]贺沛成,洪宏,伍敏敏,丁中.旱稻孢囊线虫(Heterodera elachista)孵化特性研究.植物保护,2012,38(1):101-103.Hong P C,Hong H,Wu M M,Ding Z.Study on the hatching characteristics of Heterodera elachista.Plant Prot,2012,38(1):101-103.(in Chinese with English abstract)
[7]刘炳良,孙成刚,王暄,向桂林,宋志强,高菲菲,李红梅.小麦品种对禾谷孢囊线虫(Heterodera avenae)江苏沛县群体的抗性鉴定.麦类作物学报,2012,32(3):563-568.Liu B L,Sun C G,Wang X,Xiang G L,Song Z Q,Gao FF,Li H M.Evaluation of the resistance of wheat cultivars to Peixian population of Heterodera avenae from Jiangsu Province,China.J Trit Crops,2012,32(3):563-568.(in Chinese with English abstract)
[8]Reversat G,Destombes D.Screening for resistance to Heterodera sacchari in the two cultivated rice species,Oryza sativa and O.glaberrima.Fund&Appl Nematol,1998,21(4):307-317.
[9]郑经武,程瑚瑞,方中达.土壤中线虫孢囊的三种分离方法及综合评价.植物保护,1995(1):50-51.Zheng J W,Cheng H R,Fang Z D.Three methods of isolation and comprehensive evaluation of nematode cyst in soil.Plant Prot,1995(1):50-51.Chinese with English abstract)
[10]李永宏,黄清臻.新复极差法在生物统计中的应用.医学动物防制,2002(5):270-272.Li Y H,Huang Q Z.Application of SSR on the statisties of biology.J Med Pest Control,2002(5):270-272.(in Chinese with English abstract)
[11]Stirling G,Nicol J.Advisory Services for Nematode Pests.Nematologists.org.au,1999.
[12]Soriano I R,Schmit V,Brar D S,Prot J C,Reversat G.Resistance to rice root-knot nematode Meloidogyne graminicola identified in Oryza longistaminata and O.glaberrima.Nematology,1999,1(4):395-398.
[13]丁中,Namphueng J,何旭峰,伍敏敏,洪宏.旱稻孢囊线虫生活史及侵染特性.中国水稻科学,2012,26(6):746-750.Ding Z,Namphueng J,He X F,Wu M M,Hong H.Life Cycle and infection characteristics of Rice Cyst Nematode,Heterodera elachista Ohshima in rice.Chin JRice Sci,2012,26(6):746-750.(in Chinese with English abstract)
[14]苏致衡,黄文坤,郑国栋,张宏嘉,刘淑艳,彭德良.北京地区小麦禾谷孢囊线虫病发生动态调查.植物保护,2013,39(1):116-120.Su Z H,Huang W K,Zheng G D,Zhang H J,Liu S Y,Peng D L.Investigation on the occurrence of Heterodera avenae in Beijing.Plant Prot,2013,39(1):116-120.(in Chinese with English abstract)
[15]邢小萍,袁虹霞,孙君伟,张洁,孙炳剑,李洪连.河南省小麦主推品种对2种禾谷孢囊线虫的抗性及其评价方法.作物学报,2014,40(5):805-815.Xing X P,Yuan H X,Sun J W,Zhang J,Sun B L,Li H J.Resistance to two species of cereal cyst nematode and evaluation methods in major wheat cultivars from Henan Province,China.Acta Agron Sin,2014,40(5):805-815.(in Chinese with English abstract)
[16]Seasonal fluctuation and rhizospherical distribution of population of the upland rice cyst nematode,Heterodera elachista.Jpn J Nematol,1977,7:49-57.
[2]Shimizu K.Influence of the upland rice cyst nematode,Heterodera elachista on the yield of the upland-cultured paddy rice.Jpn J Nematol,1976,6:1-6.
[3]Ding Z,Namphueng J,He X F,Peng D L,Huang W K.First report of the cyst nematode(Heterodera elachista)on rice in Hunan Province,China.Plant Dis,2011,96(1):151.
[4]Luca F D,Vovlas N,Lucarelli G,et al.Heterodera elachista the Japanese cyst nematode parasitizing corn in Northern Italy:Integrative diagnosis and bionomics.Eur J Plant Pathol,2013,136(4):857-872.
[5]王水南,彭德良,黄文坤,丁中.旱稻孢囊线虫的快速分子检测.湖南农业大学学报:自然科学版,2014,40(2):178-182.Wang S N,Peng D L,Huang W K,Ding Z.Rapid molecular diagnosis of rice cyst nematode(Heterodera elachista).J Hunan Agric Univ:Nat Sci,2014,40(2):178-182.(in Chinese with English abstract)
[6]贺沛成,洪宏,伍敏敏,丁中.旱稻孢囊线虫(Heterodera elachista)孵化特性研究.植物保护,2012,38(1):101-103.Hong P C,Hong H,Wu M M,Ding Z.Study on the hatching characteristics of Heterodera elachista.Plant Prot,2012,38(1):101-103.(in Chinese with English abstract)
[7]刘炳良,孙成刚,王暄,向桂林,宋志强,高菲菲,李红梅.小麦品种对禾谷孢囊线虫(Heterodera avenae)江苏沛县群体的抗性鉴定.麦类作物学报,2012,32(3):563-568.Liu B L,Sun C G,Wang X,Xiang G L,Song Z Q,Gao FF,Li H M.Evaluation of the resistance of wheat cultivars to Peixian population of Heterodera avenae from Jiangsu Province,China.J Trit Crops,2012,32(3):563-568.(in Chinese with English abstract)
[8]Reversat G,Destombes D.Screening for resistance to Heterodera sacchari in the two cultivated rice species,Oryza sativa and O.glaberrima.Fund&Appl Nematol,1998,21(4):307-317.
[9]郑经武,程瑚瑞,方中达.土壤中线虫孢囊的三种分离方法及综合评价.植物保护,1995(1):50-51.Zheng J W,Cheng H R,Fang Z D.Three methods of isolation and comprehensive evaluation of nematode cyst in soil.Plant Prot,1995(1):50-51.Chinese with English abstract)
[10]李永宏,黄清臻.新复极差法在生物统计中的应用.医学动物防制,2002(5):270-272.Li Y H,Huang Q Z.Application of SSR on the statisties of biology.J Med Pest Control,2002(5):270-272.(in Chinese with English abstract)
[11]Stirling G,Nicol J.Advisory Services for Nematode Pests.Nematologists.org.au,1999.
[12]Soriano I R,Schmit V,Brar D S,Prot J C,Reversat G.Resistance to rice root-knot nematode Meloidogyne graminicola identified in Oryza longistaminata and O.glaberrima.Nematology,1999,1(4):395-398.
[13]丁中,Namphueng J,何旭峰,伍敏敏,洪宏.旱稻孢囊线虫生活史及侵染特性.中国水稻科学,2012,26(6):746-750.Ding Z,Namphueng J,He X F,Wu M M,Hong H.Life Cycle and infection characteristics of Rice Cyst Nematode,Heterodera elachista Ohshima in rice.Chin JRice Sci,2012,26(6):746-750.(in Chinese with English abstract)
[14]苏致衡,黄文坤,郑国栋,张宏嘉,刘淑艳,彭德良.北京地区小麦禾谷孢囊线虫病发生动态调查.植物保护,2013,39(1):116-120.Su Z H,Huang W K,Zheng G D,Zhang H J,Liu S Y,Peng D L.Investigation on the occurrence of Heterodera avenae in Beijing.Plant Prot,2013,39(1):116-120.(in Chinese with English abstract)
[15]邢小萍,袁虹霞,孙君伟,张洁,孙炳剑,李洪连.河南省小麦主推品种对2种禾谷孢囊线虫的抗性及其评价方法.作物学报,2014,40(5):805-815.Xing X P,Yuan H X,Sun J W,Zhang J,Sun B L,Li H J.Resistance to two species of cereal cyst nematode and evaluation methods in major wheat cultivars from Henan Province,China.Acta Agron Sin,2014,40(5):805-815.(in Chinese with English abstract)
[16]Seasonal fluctuation and rhizospherical distribution of population of the upland rice cyst nematode,Heterodera elachista.Jpn J Nematol,1977,7:49-57.