葛根中葛根素与大豆苷元超声提取工艺研究
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摘要
目的优化葛根的超声提取工艺。方法采用正交试验法,以水为溶剂,以葛根素、大豆苷元为考察指标,对葛根超声提取工艺中的溶剂用量、单次提取时间及提取次数进行优化。结果葛根素进样量线性范围为0. 024~0. 160μg;稳定性的RSD为1. 00%(n=6);精密度的RSD为0. 76%(n=6);重复性的RSD为0. 80%(n=6);回收率为99. 87%,RSD为0. 70%(n=6)。大豆苷元进样量线性范围为0. 03~0. 20μg;稳定性的RSD为1. 10%(n=6);精密度的RSD为0. 76%(n=6);重复性的RSD为0. 90%(n=6);回收率为100. 32%,RSD为1. 80%(n=6)。最佳超声提取工艺为加8倍量水,提取3次,每次30 min。结论该方法简便,准确,专属性强,工艺重复性好,适于规模化生产。
Objective To optimize the ultrasonic extraction process of Puerariae lobatae Radix. Methods With the water as solvent,puerarin and daidzein as indexes,the amount of solvent,single extraction time and extraction times in the ultrasonic extraction process of Puerariae lobatae Radix were optimized by orthogonal test. Results The puerarin showed a good linear relationship in the range of0. 024-0. 160 μg,the RSD of stability was 1. 00%( n = 6),the RSD of precision was 0. 76%( n = 6),the RSD of repeatability was 0. 80%( n = 6),the recovery rate was 99. 87%,RSD was 0. 70%( n = 6). The daidzein showed a good linear relationship in the range of 0. 03-0. 20 μg,the RSD of stability was 1. 10%( n = 6),the RSD of precision was 0. 76%( n = 6),the RSD of repeatability was0. 90%( n = 6),the recovery rate was 100. 32%,RSD was 1. 80%( n = 6). The best ultrasonic extraction process was as follows: eight times the amount of water,extracting 3 times,each time 30 min. Conclusion This method is simple,accurate with strong specificity and good repeatability,which is suitable for large-scale production.
Objective To optimize the ultrasonic extraction process of Puerariae lobatae Radix. Methods With the water as solvent,puerarin and daidzein as indexes,the amount of solvent,single extraction time and extraction times in the ultrasonic extraction process of Puerariae lobatae Radix were optimized by orthogonal test. Results The puerarin showed a good linear relationship in the range of0. 024-0. 160 μg,the RSD of stability was 1. 00%( n = 6),the RSD of precision was 0. 76%( n = 6),the RSD of repeatability was 0. 80%( n = 6),the recovery rate was 99. 87%,RSD was 0. 70%( n = 6). The daidzein showed a good linear relationship in the range of 0. 03-0. 20 μg,the RSD of stability was 1. 10%( n = 6),the RSD of precision was 0. 76%( n = 6),the RSD of repeatability was0. 90%( n = 6),the recovery rate was 100. 32%,RSD was 1. 80%( n = 6). The best ultrasonic extraction process was as follows: eight times the amount of water,extracting 3 times,each time 30 min. Conclusion This method is simple,accurate with strong specificity and good repeatability,which is suitable for large-scale production.
引文
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[2]陈银霞,武占全.葛根的化学成分、药理研究和用途研究[J].科技信息,2011(30):200.
[3]王亚红,曲小姝,祝波,等.葛根素提取及纯化工艺研究现状[J].天津化工,2008,22(6):11-13.
[4]王翊臻,潘洪平.优选葛根提取工艺的实验研究[J].医学文选,2004,23(1):4-6.
[5]刘敏彦,董超,高秀强,等.中药葛根提取工艺研究[J].中国药业,2008,17(3):24-25.
[6]王美东,李常行,孙钦美.葛根提取工艺的正交设计试验[J].齐鲁药事2005,24(1):45-46.
[7]易红,杨华.葛根的品种产地和提取工艺研究进展概况[J].中国实验方剂学杂志,2006,12(11):60-63.
[8]王广峰,付海波.葛根素提取工艺的研究[J].黑龙江科技信息,2012,27(8):7.
[9]国振双,李梅,冯云生.微波法提取葛根黄酮的研究[J].齐齐哈尔大学学报,2003,19(4):14-15.
[10]公衍玲,黄山,于慧荣.葛根提取方法的比较研究及其工艺优化[J].青岛科技大学学报(自然科学版),2009,30(5):415-417.
[11]国家药典委员会.中华人民共和国药典(一部)[M].北京:中国医药科技出版社,2010:312-313.
[12]黄贵平. RP-HPLC法测定心痛平颗粒中葛根素的含量[J].现代中药研究与实践,2005,19(6):47-49.
[13]邹翔,曲中原. RP-HPLC法测定湖南粉葛中葛根素的含量[J].黑龙江医药,2011,24(4):519-520.
[14]张泓,黄庆文,王守农,等.高效液相色谱法测定冠脉通片中葛根素含量研究[J].内蒙古中医药,2010,29(8):41.
[15]白秀娟.醇溶性冻干鹿茸提取物提取方法:中国,03132527. 0[P]. 2004-03-17.