血清25(OH)D_3、hsa-miR-17-5p与HCV-RNA联合检测在丙肝疾病诊断中的意义
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摘要
目的:研究血清25(OH)D_3、hsa-miR-17-5p与HCV-RNA联合检测在丙肝疾病诊断中的意义。方法:选取我院收治的丙肝患者100例为观察组,另选我院同期体检正常者100例为对照组,观察两组研究对象血清25(OH)D_3、hsa-miR-17-5p与HCV-RNA水平、不同HCV-RNA组别患者的血清25(OH)D_3、hsa-miR-17-5p水平、HCV-RNA与血清25(OH)D_3、hsa-miR-17-5p相关性以及联合检测与单独检测对丙肝诊断效能之间的差异。结果:观察组的hsa-miR-17-5p表达量明显高于对照组,25(OH)D_3表达水平低于对照组,HCV-RNA阳性组的hsa-miR-17-5p高于HCV-RNA阴性组,25(OH)D_3低于HCV-RNA阴性组,观察组的HCV-RNA与血清25(OH)D_3、hsa-miR-17-5p分别呈现正、负相关,通过对联合检测和单独检测对丙肝患者诊断效能评估,发现血清25(OH)D_3、hsa-miR-17-5p以及HCV-RNA的临界值分别为24.23μg/L,1.89相对表达量,1.22×10~3 IU/mL。hsa-miR-17-5p+25(OH)D_3+HCV-RNA联合检测的AUC面积大于单独检测。结论:血清25(OH)D_3、hsa-miR-17-5p与HCV-RNA联合检测对于丙型肝炎诊断效能较高,灵敏度较好,建议在临床应用。
Objective:Significance of combined detection of serum 25(OH)D_3,hsa-miR-17-5p and HCV-RNA in diagnosis of hepatitis C disease.Methods:52 patients with benign ovarian tumor resection were selected from April 2016 to April 2018 in our hospital.We paid attention to the implementation of perioperative nursing intervention,and observed the prognosis and the occurrence of adverse reactions.Results:The expression of hsa-mir-17-5p in the observation group was significantly higher than that in the control group,the expression of 25(OH)D_3 was lower than that in the control group,the expression of hsa-mir-17-5p in the HCV-RNA positive group was higher than that in the HCV-RNA negative group,and the expression of 25(OH)D_3 was lower than that in the HCV-RNA negative group.The expression of HCV-RNA in the observation group was positively and negatively correlated with the expression of 25(OH)D_3 and hsa-mir-17-5p in the 25(OH)D_3 and the critical values of serum 25(OH)D_3,hsa-mir-17-5p and HCV-RNA were 24.23 ug/L,1.89 relative expression and 1.22 × 10~3 IU/mL,respectively.The area of AUC detected by hsa-miR-17-5p+ 25(OH)D_3+HCV-RNA is larger than that detected separately.Conclusion:The combined detection of serum 25(OH)D_3,hsa-mir-17-5p and HCV-RNA has high diagnostic efficiency and sensitivity for hepatitis C.It is suggested that they be used in clinical practice.
Objective:Significance of combined detection of serum 25(OH)D_3,hsa-miR-17-5p and HCV-RNA in diagnosis of hepatitis C disease.Methods:52 patients with benign ovarian tumor resection were selected from April 2016 to April 2018 in our hospital.We paid attention to the implementation of perioperative nursing intervention,and observed the prognosis and the occurrence of adverse reactions.Results:The expression of hsa-mir-17-5p in the observation group was significantly higher than that in the control group,the expression of 25(OH)D_3 was lower than that in the control group,the expression of hsa-mir-17-5p in the HCV-RNA positive group was higher than that in the HCV-RNA negative group,and the expression of 25(OH)D_3 was lower than that in the HCV-RNA negative group.The expression of HCV-RNA in the observation group was positively and negatively correlated with the expression of 25(OH)D_3 and hsa-mir-17-5p in the 25(OH)D_3 and the critical values of serum 25(OH)D_3,hsa-mir-17-5p and HCV-RNA were 24.23 ug/L,1.89 relative expression and 1.22 × 10~3 IU/mL,respectively.The area of AUC detected by hsa-miR-17-5p+ 25(OH)D_3+HCV-RNA is larger than that detected separately.Conclusion:The combined detection of serum 25(OH)D_3,hsa-mir-17-5p and HCV-RNA has high diagnostic efficiency and sensitivity for hepatitis C.It is suggested that they be used in clinical practice.
引文
1丙型肝炎患者血清hsa-miR-17-5p和hsa-miR-223-3p表达及意义[J].临床检验杂志,2016,34(5):351-353.
2季建平,欧阳小予,陈建平,等.慢性丙型肝炎患者血清25-羟维生素D3水平与肝功能、丙肝病毒载量的相关性[J].实用医学杂志,2016,32(9):1434-1437.
3季建平,陈建平,杨阳,等.慢性丙型肝炎患者血清25-羟维生素D3水平与肝纤维化和肝功能指标的相关性[J].广东医学,2016,37(5):727-729.
4 Hayes C,ChayamaK.MicroRNAs as Biomarkers for liver disease and hepatocellular carcinoma[J].International Journal of Molecular Sciences,2016,17(3):280.
5中华医学会肝病学分会.丙型肝炎防治指南[J].中华传染病杂志,2004,22(2):131-136.
6 Oksuz Z,Serin MS,Kaplan E,et al.Serum microRNAs;miR-30c-5p,miR-223-3p,miR-302c-3p and miR-17-5p could be used as novel non-invasive biomarkers for HCV-positive cirrhosis and hepatocellular carcinoma[J].Molecular Biology Reports,2015,42(3):713-720.
7 Chapman CG,Pekow J.The emerging role of miRNAs in inflammatory bowel disease:a review[J].Therap AdvGastroenterol,2015,8(1):4-22.
8 Lindner K,Haier J,Wang Z,et al.Circulating microR-NAs:emerging biomarkers for diagnosis and prognosis in patients with gastrointestinal cancers[J].Clinical Science,2015,128(1):1.
9 Latorre I,Leidinger P,Backes C,et al.A novel wholeblood miRNA signature for a rapid diagnosis of pulmonary tuberculosis.[J].European Respiratory Journal,2015,45(4):1173-1176.
10 Naito Y,Tanaka Y,Ochiya T.microRNAs and Hepatitis B//microRNA:Medical Evidence[M].Springer International Publishing,2015.
11 Lambert ND.Polymorphisms in HLA-DPB1are associated with differences in rubella-specific humoral immunity after vaccination[J].Journal of Infectious Diseases,2015,211(6):898-905.
12焦方舟,张海月,张文斌,等.血清25(OH)D3水平与慢性丙型肝炎相关性的Meta分析[J].疑难病杂志,2017,16(8):825-829.
13张苹,李杰,査显丰,等.丙型肝炎患者血清hsa-miR-17-5p和hsa-miR-223-3p表达及意义[J].临床检验杂志,2016,34(5):351-353.
14汪艳.丙型肝炎患者HCV RNA载量、肝脏纤维化与25羟基维生素D水平的相关性分析[J].安徽医药,2017,21(2).
15汪艳,陈乐.丙型肝炎病人丙肝病毒RNA载量、肝脏纤维化与25羟基维生素D水平的相关性分析[J].安徽医药,2017,21(2):300-302.
2季建平,欧阳小予,陈建平,等.慢性丙型肝炎患者血清25-羟维生素D3水平与肝功能、丙肝病毒载量的相关性[J].实用医学杂志,2016,32(9):1434-1437.
3季建平,陈建平,杨阳,等.慢性丙型肝炎患者血清25-羟维生素D3水平与肝纤维化和肝功能指标的相关性[J].广东医学,2016,37(5):727-729.
4 Hayes C,ChayamaK.MicroRNAs as Biomarkers for liver disease and hepatocellular carcinoma[J].International Journal of Molecular Sciences,2016,17(3):280.
5中华医学会肝病学分会.丙型肝炎防治指南[J].中华传染病杂志,2004,22(2):131-136.
6 Oksuz Z,Serin MS,Kaplan E,et al.Serum microRNAs;miR-30c-5p,miR-223-3p,miR-302c-3p and miR-17-5p could be used as novel non-invasive biomarkers for HCV-positive cirrhosis and hepatocellular carcinoma[J].Molecular Biology Reports,2015,42(3):713-720.
7 Chapman CG,Pekow J.The emerging role of miRNAs in inflammatory bowel disease:a review[J].Therap AdvGastroenterol,2015,8(1):4-22.
8 Lindner K,Haier J,Wang Z,et al.Circulating microR-NAs:emerging biomarkers for diagnosis and prognosis in patients with gastrointestinal cancers[J].Clinical Science,2015,128(1):1.
9 Latorre I,Leidinger P,Backes C,et al.A novel wholeblood miRNA signature for a rapid diagnosis of pulmonary tuberculosis.[J].European Respiratory Journal,2015,45(4):1173-1176.
10 Naito Y,Tanaka Y,Ochiya T.microRNAs and Hepatitis B//microRNA:Medical Evidence[M].Springer International Publishing,2015.
11 Lambert ND.Polymorphisms in HLA-DPB1are associated with differences in rubella-specific humoral immunity after vaccination[J].Journal of Infectious Diseases,2015,211(6):898-905.
12焦方舟,张海月,张文斌,等.血清25(OH)D3水平与慢性丙型肝炎相关性的Meta分析[J].疑难病杂志,2017,16(8):825-829.
13张苹,李杰,査显丰,等.丙型肝炎患者血清hsa-miR-17-5p和hsa-miR-223-3p表达及意义[J].临床检验杂志,2016,34(5):351-353.
14汪艳.丙型肝炎患者HCV RNA载量、肝脏纤维化与25羟基维生素D水平的相关性分析[J].安徽医药,2017,21(2).
15汪艳,陈乐.丙型肝炎病人丙肝病毒RNA载量、肝脏纤维化与25羟基维生素D水平的相关性分析[J].安徽医药,2017,21(2):300-302.