非创伤性股骨头坏死患者记忆B细胞和浆母细胞及血清免疫球蛋白变化
摘要
目的探讨老年非创伤性股骨头坏死(NONFH)患者外周血中记忆B细胞和浆母细胞及血清免疫球蛋白(Ig)的变化及其在发病机制中的作用。方法流式细胞仪测定NONFH患者(NONFH组)和健康对照(HC)组外周血中B细胞和浆母细胞各亚群数量的变化。微量样本多指标流式蛋白定量技术检测IgG、IgA和IgM的水平。结果与HC组比较,NONFH组外周血中IgD~+CD27~-B细胞和IgD~-CD27~-B细胞显著增高(均P<0.001),而IgD~+CD27~+B细胞和IgG~+IgD~-CD27~+B细胞明显减少(P<0.001,P=0.006)。NONFH组CD38~+、CD38~+CD138~+、CD38~+IgM~+、CD38~+IgG~+浆母细胞数量、血清IgG水平显著高于HC组(P<0.001)。NONFH组IgG~+IgD~-CD27~+B细胞与血清IgG、CD38~+CD138~+浆母细胞和IgG~+CD38~+浆母细胞数量呈负相关。血清IgG水平与CD38~+CD138~+浆母细胞和IgG~+CD38~+浆母细胞的数量呈正相关。结论老年NONFH患者外周血中记忆B细胞数量减少,浆母细胞和血清IgG免疫球蛋白水平显著增高,与疾病的进展具有相关性,在老年NONFH免疫学过程中发挥着重要作用。
引文
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13 Tarlton NJ,Green CM,Lazarus NH,et al.Plasmablast frequency and trafficking receptor expression are altered in pediatric ulcerative colitis[J].Inflamm Bowel Dis,2012;18(12):2381-91.
14 Tangye SG,Liu YJ,Aversa G,et al.Identification of Functional Human Splenic Memory B Cells by Expression of CD148 and CD27[J].J Exp Med,1998;188(9):1691-703.
2张立智,蒋垚.骨免疫学研究进展[J].国际骨科学杂志,2009;30(4):218-20.
3 Niu XY,Zhang HY,Liu YJ,et al.Peripheral B-cell activation and exhaustion markers in patients with ankylosing spondylitis[J].Life Sci,2013;93(18-19):687-92.
4 Ficat RP.Idiopathic bone necrosis of the femoral head.Early diagnosis and treatment[J].J Bone Joint Surg Br,1985;67(1):3-9.
5朱建辛,王跃,邓骏才,等.病灶清除后打压植骨联合多孔钽金属棒植入治疗早期股骨头缺血性坏死[J].中国修复重建外科杂志,2011;25(11):1335-8.
6 Kim HS,Bae SC,Kim TH,et al.Endothelial nitric oxide synthase gene polymorphisms and the risk of osteonecrosis of the femoral head in systemic lupus erythematosus[J].Int Orthop,2013;37(11):2289-96.
7 van Vugt RM,Sijbrandij ES,Bijlsma JW.Magnetic resonance imaging of the femoral head to detect avascular necrosis in active rheumatoid arthritis treated with methylprednisolone pulse therapy[J].Scand J Rheumatol,1996;25(2):74-6.
8 Allman D,Pillai S.Peripheral B cell subsets[J].Curr Opin Immunol,2008;20(2):149-57.
9 Di Sabatino A,Rosado MM,Cazzola P,et al.Splenic function and Ig M-memory B cells in Crohn's disease patients treated with infliximab[J].Inflamm Bowel Dis,2008;14(5):591-6.
10 Di Sabatino A,Rosado MM,Ciccocioppo R,et al.Depletion of immunoglobulin M memory B cells is associated with splenic hypofunction in inflammatory bowel disease[J].Am J Gastroenterol,2005;100(8):1788-95.
11 Jinno Y,Ohtani H,Nakamura S,et al.Infiltration of CD19+plasma cells with frequent labeling of Ki-67 in corticosteroid-resistant active ulcerative colitis[J].Virchows Arch,2006;448(4):412-21.
12 Hosomi S,Oshitani N,Kamata N,et al.Increased numbers of immature plasma cells in peripheral blood specifically overexpress chemokine receptor CXCR3 and CXCR4 in patients with ulcerative colitis[J].Clin Exp Immunol,2011;163(2):215-24.
13 Tarlton NJ,Green CM,Lazarus NH,et al.Plasmablast frequency and trafficking receptor expression are altered in pediatric ulcerative colitis[J].Inflamm Bowel Dis,2012;18(12):2381-91.
14 Tangye SG,Liu YJ,Aversa G,et al.Identification of Functional Human Splenic Memory B Cells by Expression of CD148 and CD27[J].J Exp Med,1998;188(9):1691-703.