红芪胶囊对低剂量辐射损伤小鼠的保护作用
|
摘要
目的:采用低剂量辐射损伤小鼠模型,观察红芪胶囊的药理作用。方法:二级昆明种小鼠216只,除正常组36只外,其余180只采用多次低剂量累积辐射。将照射的180只小鼠按体重随机分为5组,每组12只,分别为:模型组,阳性对照组(1.20 g/kg),红芪胶囊小剂量组(0.175 g/kg),红芪胶囊中剂量组(0.35 g/kg),红芪胶囊大剂量组(0.70 g/kg);将未照射的36只小鼠设为正常组,每组又分为给药6、12、24 d三个时间点,每时间点12只动物。各组在末次照射当天分别灌胃上述药物,正常对照组及模型对照组灌胃等量蒸馏水,每日一次,连续给药24 d,观察红芪胶囊的药理作用。结果:1红芪胶囊给药12 d、24 d对辐射损伤小鼠的外周血象红细胞(RBC)、白细胞(WBC)、血小板(PLT)和血红蛋白含量(HGB)有明显的升高作用(P<0.01或P<0.05);2明显增加辐射损伤小鼠的肝脏、脾脏、大脑和睾丸组织的湿质量(P<0.01或P<0.05);3镜下观察可见红芪胶囊能明显改善损伤小鼠骨髓细胞活性。结论:红芪胶囊对低剂量辐射损伤具有一定保护作用。
Objective: To investigate the pharmacological effects of Hongqi capsule on low-dose radiation injured mice. Methods:216 Kunming mice were divided into normal group and experiment group. There were 36 mice in the normal group, while 180 in the experiment group were irradiated by multiple low-dose radiation, and then they were randomly divided into five groups of 12 each:the model group, the positive control group(1.20g/kg), Hongqi capsule small dose group(0.175g/kg), Hongqi capsule middle dose group(0.35g/kg), and Hongqi capsule high-dose group(0.70g/kg); 36 non-irradiated mice were divided into the normal group, each group was administrated for 6, 12, 24 days, respectively. Every group was administered the drugs on the day of the last irradiation, and the normal control group and model control group were given distilled water, once-daily. The pharmacological effects of Hongqi capsule after continuous administration of 24 days were detected. Results: 1 Hongqi capsule increased the red blood cell(RBC), white blood cell(WBC), platelet(PLT) and hemoglobin content(HGB) of radiation injured mice in peripheral blood after administration for 12 and 24 days.(P<0.01 or P<0.05); 2Hongqi capsule increased the wet weight of liver, spleen, brain and testicular tissue(P<0.01 or P<0.05); 3Hongqi capsule increased improved marrow cell activity in microscope. Conclusions: Hongqi capsule certainly has protective effect on low-dose injured mice.
Objective: To investigate the pharmacological effects of Hongqi capsule on low-dose radiation injured mice. Methods:216 Kunming mice were divided into normal group and experiment group. There were 36 mice in the normal group, while 180 in the experiment group were irradiated by multiple low-dose radiation, and then they were randomly divided into five groups of 12 each:the model group, the positive control group(1.20g/kg), Hongqi capsule small dose group(0.175g/kg), Hongqi capsule middle dose group(0.35g/kg), and Hongqi capsule high-dose group(0.70g/kg); 36 non-irradiated mice were divided into the normal group, each group was administrated for 6, 12, 24 days, respectively. Every group was administered the drugs on the day of the last irradiation, and the normal control group and model control group were given distilled water, once-daily. The pharmacological effects of Hongqi capsule after continuous administration of 24 days were detected. Results: 1 Hongqi capsule increased the red blood cell(RBC), white blood cell(WBC), platelet(PLT) and hemoglobin content(HGB) of radiation injured mice in peripheral blood after administration for 12 and 24 days.(P<0.01 or P<0.05); 2Hongqi capsule increased the wet weight of liver, spleen, brain and testicular tissue(P<0.01 or P<0.05); 3Hongqi capsule increased improved marrow cell activity in microscope. Conclusions: Hongqi capsule certainly has protective effect on low-dose injured mice.
引文
[1]赵航,贺中民,涂宏海,等.小鼠低剂量辐射损伤模型的初步研究[J].现代生物医学进展,2012,12(20):3817-3821Zhao Hang,He Zhong-min,Tu Hong-hai,et al.Research of the Model of Low Dose Radiation-injured Mice[J].Progress in Modern Biomedicine,2012,12(20):3817-3821
[2]尹虹,林丽玉.长期低剂量电离辐射对放射工作人员血细胞参数的影响[J].浙江预防医学,2010,22(6):51-52Yin Hong,Lin Li-yu.Long-term low doses of ionizing radiation effects on blood cell parameters of radiation workers[J].Zhejiang Journal of Preventive Medicine,2010,22(6):51-52
[3]庄晓玲,方汉波,徐岳军,等.长期低剂量电离辐射对男性放射人员血象的影响[J].浙江预防医学,2009,21(7):38-39Zhuang Xiao-ling,Fang Han-bo,Xu Yue-jun,et al.Long-term low doses of ionizing radiation effects on male radiation personnel blood picture[J].Zhejiang Journal of Preventive Medicine,2009,21(7):38-39
[4]李予蓉,谭娟,徐胜龙,等.红芪胶囊对辐射损伤大鼠的药效作用研究[J].解放军药学学报,2010,26(5):400-402Li Yu-rong,Tan Juan,Xu Sheng-long,et al.The Radiation-protective Effect of Radix Hedysari Capsules on Mice[J].Pharmaceutical Journal of Chinese People's Liberation Army,2010,26(5):400-402
[5]李予蓉,谭娟,王晋,等.红芪胶囊对60Co-γ射线辐射后大鼠脑、肺、血清SOD活性和MDA含量的影响[J].陕西中医,2010,31(3):363-364Li Yu-rong,Tan Juan,Wang Jin,et al.The Effects of Radix Hedysari Capsules on the activities of SOD and MDA in rat brain,lung and serunm after exposured under60Co-γray[J].Shaanxi Journal of Traditional Chinese Medicine,2010,31(3):363-364
[6]李德冠,王月英,吴红英,等.电离辐射对不同品系小鼠造血功能的影响[J].中国辐射卫生,2010,19(3):261-262Li De-guan,Wang Yue-ying,Wu Hong-ying,et al.The Effect of Radiation on Hematopoiesis in Three Kinds of Mouse[J].Chi nese Journal of Radiological Health,2010,19(3):261-262
[7]郭月凤,张慧芳,杨彪,等.急性辐射损伤的生物剂量估算方法[J].辐射研究与辐射工艺学报,2012,30(3):135-141Guo Yue-feng,Zhang Hui-fang,Yang Biao,et al.Biodosimetry estimation methods for acute radiation damage[J].Journal of Radiation Research and Radiation Processing,2012,30(3):135-141
[8]何火聪,吴君心,苏颖,等.X射线致小鼠急性损伤的观察[J].辐射研究与辐射工艺学报,2012,30(2):125-128He Huo-cong,Wu Jun-xin,Su Ying,et al.Observation of acute radiation damage on mice exposed to X-rays[J].Journal of Radiation Research and Radiation Processing,2012,30(2):125-128
[9]接亚敏,韩北秋,乔文波,等.放射性肺损伤相关临床因素的研究[J].中华肿瘤防治杂志,2011,18(15):1201-1203Jie Ya-min,Han Bei-qiu,Qiao Wen-bo,et al.Investigation of clinical correlation factors for radiation induced lung injury[J].Chinese Journal of Cancer Prevention and Treatment,2011,18(15):1201-1203
[10]郝娜,程红缨,任为,等.电磁辐射损伤机制与防护[J].现代生物医学进展,2012,12(13):2568-2572Hao Na,Cheng Hong-ying,Ren Wei,et al.The Damage Mechanism and Protection of Electromagnetic Radiation[J].Progress in Modern Biomedicine,2012,12(13):2568-2572
[11]叶江枫,袁名辉.辐射预防的研究进展[J].成都医学院学报,2012,7(02Z):277Ye Jiang-feng,Yuan Ming-hui.Research Progress of Radiation Prevention[J].Journal of Cheng Du Medical College,2012,7(02Z):277
[12]孟凡旭,单玉兴,董娟聪等.不同剂量电离辐射对小鼠脾脏调节性T细胞及TGF-β1表达的影响[J].吉林大学学报:医学版,2011,37(3):398-402Meng Fan-xu,Shan Yu-xing,Dong Juan-cong,et al.Effects of ionizing radiation with different doses on regulatory T cells and expression of TGF-β1 in mouse spleen[J].Journal of Jilin University:Med Ed,2011,37(3):398-402
[13]许芸,邬蒙.低剂量辐射诱导小鼠骨髓单个核细胞γH2AX形成的时间剂量效应[J].实用癌症杂志,2009,24(4):333-336,354Xu Yun,Wu Meng.Dose-response and Time-effect Betweenγ-rays Radiation and Histone H2AX Phosphorylation(γHZAX)of Mononuclear Bone Marrow Cell[J].The Practical Journal of Cancer,2009,24(4):333-336,354
[14]田娟,张元珍,周春.移动电话电磁辐射对仔鼠脑组织SOD活力、MDA含量的影响[J].武汉大学学报(医学版),2006,27(2):160-162,166Tian Juan,Zhang Yuan-zhen,Zhou Chun.Effect of Electromagnetic Radiation of Cell Phone on Content of SOD and MDA in Offspring's Brain Tissue of Mice[J].Medical Journal of Wuhan University,2006,27(2):160-162,166
[15]龙静,张红,刘阳,等.12C6+照射对小鼠睾丸组织脂质过氧化、SOD活性及细胞周期的影响[J].核技术,2008,31(6):445-448Long Jing,Zhang Hong,Liu Yang,et al.Effects of 12C6+irradiation of mouse abdomen on lipid peroxidation,SOD activities and cell cycle of testis[J].Nuclear Techniques,2008,31(6):445-448
[16]谢怀江,宋聚忠,寇庆河,等.寒冷复合电离辐射对大鼠脏器重量及精子数量的影响[J].沈阳部队医药,2001,14(1):36-37Xie Huai-jiang,Song Ju-zhong,Kou Qing-he,et al.Cold compound ionizing radiation effects on rat viscera weight and sperm count[J].Shenyang Force Medical,2001,14(1):36-37
[17]姜义,肖雪媛,关桂梅,等.红景天素对X射线照射小鼠的预防作用[J].中华放射医学与防护杂志,1995,15(3):214Jiang Yi,Xiao Xue-yuan,Guan Gui-mei,et al.The Prevent Effects of Rhodiosin on X-ray ionizing radiated Mice[J].Chinese Journal of Radiological Medicine and Protection,1995,15(3):214
[18]贾正平,谢景文,魏虎来,等.狭叶红景天水提物的辐射保护作用[J].兰州医学院学报,1997,23(3):17-19Jia Zheng-ping,Xie Jing-wen,Wei Hu-lai,et al.Protective effects of R.Kirilowii on radiation[J].Journal of Lanzhou Medical College,1997,23(3):17-19
[19]王庆宾,韩婷,孙连娜,等.抗辐射的天然药物研究进展察[J].药学实践杂志,2012,30(3):171-174,177Wang Qing-bin,Han Ting,Sun Lian-na,et al.Progress on anti-radiation natural medicines[J].The Journal of Pharmaceutical Practice,2012,30(3):171-174,177
[20]邹佳,宋海峰.抗氧化药物在辐射损伤防治研究中的新进展[J].辐射研究与辐射工艺学报,2012,30(2):142-147Zou Jia,Song Hai-feng.The development of antioxidants in radioprotective research[J].Journal of Radiation Research and Radiation Processing,2012,30(2):142-147
[2]尹虹,林丽玉.长期低剂量电离辐射对放射工作人员血细胞参数的影响[J].浙江预防医学,2010,22(6):51-52Yin Hong,Lin Li-yu.Long-term low doses of ionizing radiation effects on blood cell parameters of radiation workers[J].Zhejiang Journal of Preventive Medicine,2010,22(6):51-52
[3]庄晓玲,方汉波,徐岳军,等.长期低剂量电离辐射对男性放射人员血象的影响[J].浙江预防医学,2009,21(7):38-39Zhuang Xiao-ling,Fang Han-bo,Xu Yue-jun,et al.Long-term low doses of ionizing radiation effects on male radiation personnel blood picture[J].Zhejiang Journal of Preventive Medicine,2009,21(7):38-39
[4]李予蓉,谭娟,徐胜龙,等.红芪胶囊对辐射损伤大鼠的药效作用研究[J].解放军药学学报,2010,26(5):400-402Li Yu-rong,Tan Juan,Xu Sheng-long,et al.The Radiation-protective Effect of Radix Hedysari Capsules on Mice[J].Pharmaceutical Journal of Chinese People's Liberation Army,2010,26(5):400-402
[5]李予蓉,谭娟,王晋,等.红芪胶囊对60Co-γ射线辐射后大鼠脑、肺、血清SOD活性和MDA含量的影响[J].陕西中医,2010,31(3):363-364Li Yu-rong,Tan Juan,Wang Jin,et al.The Effects of Radix Hedysari Capsules on the activities of SOD and MDA in rat brain,lung and serunm after exposured under60Co-γray[J].Shaanxi Journal of Traditional Chinese Medicine,2010,31(3):363-364
[6]李德冠,王月英,吴红英,等.电离辐射对不同品系小鼠造血功能的影响[J].中国辐射卫生,2010,19(3):261-262Li De-guan,Wang Yue-ying,Wu Hong-ying,et al.The Effect of Radiation on Hematopoiesis in Three Kinds of Mouse[J].Chi nese Journal of Radiological Health,2010,19(3):261-262
[7]郭月凤,张慧芳,杨彪,等.急性辐射损伤的生物剂量估算方法[J].辐射研究与辐射工艺学报,2012,30(3):135-141Guo Yue-feng,Zhang Hui-fang,Yang Biao,et al.Biodosimetry estimation methods for acute radiation damage[J].Journal of Radiation Research and Radiation Processing,2012,30(3):135-141
[8]何火聪,吴君心,苏颖,等.X射线致小鼠急性损伤的观察[J].辐射研究与辐射工艺学报,2012,30(2):125-128He Huo-cong,Wu Jun-xin,Su Ying,et al.Observation of acute radiation damage on mice exposed to X-rays[J].Journal of Radiation Research and Radiation Processing,2012,30(2):125-128
[9]接亚敏,韩北秋,乔文波,等.放射性肺损伤相关临床因素的研究[J].中华肿瘤防治杂志,2011,18(15):1201-1203Jie Ya-min,Han Bei-qiu,Qiao Wen-bo,et al.Investigation of clinical correlation factors for radiation induced lung injury[J].Chinese Journal of Cancer Prevention and Treatment,2011,18(15):1201-1203
[10]郝娜,程红缨,任为,等.电磁辐射损伤机制与防护[J].现代生物医学进展,2012,12(13):2568-2572Hao Na,Cheng Hong-ying,Ren Wei,et al.The Damage Mechanism and Protection of Electromagnetic Radiation[J].Progress in Modern Biomedicine,2012,12(13):2568-2572
[11]叶江枫,袁名辉.辐射预防的研究进展[J].成都医学院学报,2012,7(02Z):277Ye Jiang-feng,Yuan Ming-hui.Research Progress of Radiation Prevention[J].Journal of Cheng Du Medical College,2012,7(02Z):277
[12]孟凡旭,单玉兴,董娟聪等.不同剂量电离辐射对小鼠脾脏调节性T细胞及TGF-β1表达的影响[J].吉林大学学报:医学版,2011,37(3):398-402Meng Fan-xu,Shan Yu-xing,Dong Juan-cong,et al.Effects of ionizing radiation with different doses on regulatory T cells and expression of TGF-β1 in mouse spleen[J].Journal of Jilin University:Med Ed,2011,37(3):398-402
[13]许芸,邬蒙.低剂量辐射诱导小鼠骨髓单个核细胞γH2AX形成的时间剂量效应[J].实用癌症杂志,2009,24(4):333-336,354Xu Yun,Wu Meng.Dose-response and Time-effect Betweenγ-rays Radiation and Histone H2AX Phosphorylation(γHZAX)of Mononuclear Bone Marrow Cell[J].The Practical Journal of Cancer,2009,24(4):333-336,354
[14]田娟,张元珍,周春.移动电话电磁辐射对仔鼠脑组织SOD活力、MDA含量的影响[J].武汉大学学报(医学版),2006,27(2):160-162,166Tian Juan,Zhang Yuan-zhen,Zhou Chun.Effect of Electromagnetic Radiation of Cell Phone on Content of SOD and MDA in Offspring's Brain Tissue of Mice[J].Medical Journal of Wuhan University,2006,27(2):160-162,166
[15]龙静,张红,刘阳,等.12C6+照射对小鼠睾丸组织脂质过氧化、SOD活性及细胞周期的影响[J].核技术,2008,31(6):445-448Long Jing,Zhang Hong,Liu Yang,et al.Effects of 12C6+irradiation of mouse abdomen on lipid peroxidation,SOD activities and cell cycle of testis[J].Nuclear Techniques,2008,31(6):445-448
[16]谢怀江,宋聚忠,寇庆河,等.寒冷复合电离辐射对大鼠脏器重量及精子数量的影响[J].沈阳部队医药,2001,14(1):36-37Xie Huai-jiang,Song Ju-zhong,Kou Qing-he,et al.Cold compound ionizing radiation effects on rat viscera weight and sperm count[J].Shenyang Force Medical,2001,14(1):36-37
[17]姜义,肖雪媛,关桂梅,等.红景天素对X射线照射小鼠的预防作用[J].中华放射医学与防护杂志,1995,15(3):214Jiang Yi,Xiao Xue-yuan,Guan Gui-mei,et al.The Prevent Effects of Rhodiosin on X-ray ionizing radiated Mice[J].Chinese Journal of Radiological Medicine and Protection,1995,15(3):214
[18]贾正平,谢景文,魏虎来,等.狭叶红景天水提物的辐射保护作用[J].兰州医学院学报,1997,23(3):17-19Jia Zheng-ping,Xie Jing-wen,Wei Hu-lai,et al.Protective effects of R.Kirilowii on radiation[J].Journal of Lanzhou Medical College,1997,23(3):17-19
[19]王庆宾,韩婷,孙连娜,等.抗辐射的天然药物研究进展察[J].药学实践杂志,2012,30(3):171-174,177Wang Qing-bin,Han Ting,Sun Lian-na,et al.Progress on anti-radiation natural medicines[J].The Journal of Pharmaceutical Practice,2012,30(3):171-174,177
[20]邹佳,宋海峰.抗氧化药物在辐射损伤防治研究中的新进展[J].辐射研究与辐射工艺学报,2012,30(2):142-147Zou Jia,Song Hai-feng.The development of antioxidants in radioprotective research[J].Journal of Radiation Research and Radiation Processing,2012,30(2):142-147