双酚A与6月~2岁婴幼儿女童乳房发育的关系研究
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摘要
目的研究环境内分泌干扰物双酚A(bisphenol A,BPA)与2岁以下婴幼儿女童乳房发育(permature thelarche,PT)的关系。方法选择6月~2岁PT 40例,正常同龄女童32例,利用高效液相色谱串联质谱法(HPLC-MSMS)进行血清BPA浓度测定,同时运用化学发光法进行血清性激素浓度:卵泡刺激素(follicule-stimulating hormone,FSH)、黄体生成素(luteinizing hormone,LH)、雌二醇(estradiol,E2)、泌乳素(prolactin,PRL)测定,比较两组差异及分析BPA与性激素之间相关性。结果6月~2岁婴幼儿女童PT组血清BPA检出率及浓度均显著高于对照组(P均<0.01)。两组血清FSH,PRL浓度差异无统计学意义(P>0.05),PT组血清E2浓度高于对照组(P<0.05),PT组血清LH浓度高于对照组(P<0.01)。两组血清BPA浓度与FSH浓度、PRL浓度无相关性(P>0.05)。血清BPA浓度与E2和LH浓度有相关性(P<0.01)。结论 6月~2岁婴幼儿女童存在BPA的暴露;BPA暴露可能是6月~2岁婴幼儿女童出现PT的原因之一。6月~2岁婴幼儿女童BPA的暴露可能影响LH及E2的分泌。
Objective To investigate the association between bisphenol A(BPA)and permature thelarche in infant girls aged 6months to 2years old. Methods The blood samples were collected from 40 infants aged 6months to 2years old with PT and 32 normal femal infants.The concentration of BPA in serum was determine by using HPLC-MS-MS.The concentration of sex hormones follicule-stimulating hormone(FSH),luteinizing hormone(LH),estradiol(E2),prolactin(PRL)in serum were detected by chemiluminescence method at the same time.The difference between the two groups and the correlation between BPA and sex hormones were analysed. Results The relevance ratio and concentration of BPA in PT group were higher than those of the control group(P<0.01).The concentrations of FSH,PRL in the two groups had no statistically significant differences(P>0.05).The concentrations of E2 in PT group was higher than that of the control group(P<0.05).The concentrations of LH of PT group was higher than that of the control group(P<0.01).There were no correlation between the concentrations of BPA and FSH,PRL(P>0.05).There were correlations between the concentrations of BPA and LH,E2(P<0.01). Conclusions 6months~2years old infant girls are under the existence of BPA exposure.BPA exposure is likely one of the causes of PT for 6months~2years old infant girls.BPA exposure maybe affect the secretions of LH and E2.
Objective To investigate the association between bisphenol A(BPA)and permature thelarche in infant girls aged 6months to 2years old. Methods The blood samples were collected from 40 infants aged 6months to 2years old with PT and 32 normal femal infants.The concentration of BPA in serum was determine by using HPLC-MS-MS.The concentration of sex hormones follicule-stimulating hormone(FSH),luteinizing hormone(LH),estradiol(E2),prolactin(PRL)in serum were detected by chemiluminescence method at the same time.The difference between the two groups and the correlation between BPA and sex hormones were analysed. Results The relevance ratio and concentration of BPA in PT group were higher than those of the control group(P<0.01).The concentrations of FSH,PRL in the two groups had no statistically significant differences(P>0.05).The concentrations of E2 in PT group was higher than that of the control group(P<0.05).The concentrations of LH of PT group was higher than that of the control group(P<0.01).There were no correlation between the concentrations of BPA and FSH,PRL(P>0.05).There were correlations between the concentrations of BPA and LH,E2(P<0.01). Conclusions 6months~2years old infant girls are under the existence of BPA exposure.BPA exposure is likely one of the causes of PT for 6months~2years old infant girls.BPA exposure maybe affect the secretions of LH and E2.
引文
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[12]薛颖,于宝生.Kiss-1基因在双酚A诱导的雌性性早熟大鼠下丘脑中的表达[J].南京医科大学学报,2007,27(12):1377-1379.
[13]Losa-Ward SM,Todd KL,McCaffrey KA,et al.Disrupted organization of rfamide pathways in the hypothalamus is associated with advanced puberty in female rats neonatally exposed to bisphenol A[J].Biology of Reproduction,2012,87(2):28-28.
[14]Adewale HB,Jefferson WN,Newbold RR,et al.Neonatal bisphenol-A expo-sure alters rat reproductive development and ovarian morphology without impairing activation of gonadotropin releasing hormone neurons[J].Biol Reprod,2009,81(4):690-699.
[2]季钗,潘斌华,王旭,等.健康婴儿LH、FSH、雌二醇与睾酮的血清浓度[J].中华内分泌代谢杂志,2005,12(21):514-517.
[3]Gssler N,Peuschel T,Pankau R.Pediatric reference estradiol,testosterone values of lutropin,follitropin and prolactin[J].Clin Lab,2000,46(11-12):553-560.
[4]Rubin BS.Bisphenol A:an endocrine disruptor with widespread exposure and multiple effects[J].Steroid Biochem Mol Biol,2011,127(1-2):27-34.
[5]Patisaul HB,Adewale HB.Long-term effects of environmental endocrine disruptors on reproductive physiology and behavior[J].Frontiers in Behavioral Neuroscience,2009,3(6):10-15.
[6]Spanier AJ,Kahn RS,Kunselman AR,et al.Prenatal exposure to bisphenol A and child wheeze from birth to 3years of age[J].Environmental Health Perspectives,2012,120(6):916-920.
[7]Roy JR,Chakraborty S,Chakraborty TR.Estrogen-like endocrine disrupting chemicals affecting puberty in humans-a review[J].Med Sci Monit,2009,15(6):137-1345.
[8]杨玉,王荻兰,卢庆红,等.婴幼儿女童乳房早发育与内分泌干扰物相关性分析[J].国际内分泌代谢杂志,2014,34(1):1-4.
[9]Curfman AL,Reljanovic SM,McNelis KM,et al.Premature thelarche in infants and toddlers:prevalence,natural history and environmental determinants[J].Pediatr Adolesc Gynecol,2011,24(6):338-341.
[10]Richter CA,Taylor JA,Ruhlen RL,et al.Estradiol and Bisphenol A stimulate androgen receptor and estrogen receptor gene expression in fetal mouse prostate mesenchyme cells[J].Environ Health Perspect,2007,115(6):902-908.
[11]Okuda K,Takiguchi M,Yoshihara S.In vivo estrogenic potential of 4-methyl-2,4-bis(4-hydroxyphenyl)pent-1-ene,an active metabolite of bisphenol A,in uterus of ovariectomized rat[J].Toxicol Lett,2010,197(1):7-11.
[12]薛颖,于宝生.Kiss-1基因在双酚A诱导的雌性性早熟大鼠下丘脑中的表达[J].南京医科大学学报,2007,27(12):1377-1379.
[13]Losa-Ward SM,Todd KL,McCaffrey KA,et al.Disrupted organization of rfamide pathways in the hypothalamus is associated with advanced puberty in female rats neonatally exposed to bisphenol A[J].Biology of Reproduction,2012,87(2):28-28.
[14]Adewale HB,Jefferson WN,Newbold RR,et al.Neonatal bisphenol-A expo-sure alters rat reproductive development and ovarian morphology without impairing activation of gonadotropin releasing hormone neurons[J].Biol Reprod,2009,81(4):690-699.