电针疗法对缺血性脑卒中大鼠肢体肌张力增高抑制作用及对中枢系统GABA能中间神经元表达的影响
摘要
目的分析电针疗法对缺血性脑卒中大鼠肢体肌张力增高抑制作用及对中枢系统γ-氨基丁酸(GABA)能中间神经元表达的影响。方法选取由本院实验动物中心提供的健康雄性SD大鼠30只,制备大鼠右侧大脑中动脉阻塞(MCAO)模型,使用G6805多通路电针仪对观察组大鼠在造模后第3日行电针治疗,每次30 min,每日1次,共进行6 d;观察大鼠治疗前后神经受损程度,观察治疗前后大鼠电生理描记肌张力状况,免疫组化检测大鼠微白蛋白(PV)、钙结合蛋白D-28K(CB),使用Motic Images Advanced分析软件检测其积分光密度数值。结果治疗后观察组大鼠NSS评分较治疗前、对照组显著降低,电生理描记肌张力较治疗前、对照组显著升高,差异有统计学意义(P<0.05);治疗后观察组大鼠脑干、颈膨大及腰膨大部位CB表达积分密度值均高于对照组,差异有统计学意义(P<0.05);治疗后观察组大鼠脑干、颈膨大及腰膨大部位PV表达积分密度值均高于对照组,差异有统计学意义(P<0.05)。结论电针治疗可提升缺血性脑卒中大鼠钙结合蛋白PV、CD表达,缓解其肢体痉挛状况。
引文
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[2]王二争,李佩芳.电针拮抗肌运动点配合康复训练治疗脑卒中后痉挛状态的临床观察[J].安徽医药,2016,20(7):1317-1320.
[3]Prager EM,Bergstrom HC,Wynn GH,et al.The basolateral amygdalaγ-aminobutyric acidergic system in health and disease[J].Journal of Neuroscience Research,2016,94(6):548-552.
[4]盛国滨,刘洋,刘刚,等.电针治疗脑卒中痉挛期膝过伸的临床疗效观察[J].针灸临床杂志,2017,33(3):28-31.
[5]赵浩,李永宁,王任直,等.大鼠局灶性脑缺血模型的有效制备[J].中国实验动物学报,2009,17(6):432-436.
[6]李忠仁.实验针灸学[M].2版.北京:中国中医药出版社,2007:154-157.
[7]Chen JL,Sanberg PR,Li Y,et al.Intarvenous administration of human umbilical cord blood redcues behavioral deficits after stroke in rats[J].Stroke,2001,32:2682-2688.
[8]Clark MQ,Mccumsey SJ,Lopezdarwin S,et al.Functional genetic screen to identify interneurons governing behaviorally distinct aspects of drosophila larval motor programs[J].G3Genesgenetics,2016,6(7):2023-2031.
[9]王雪梅,李怡,牟杨,等.缪刺治疗脑卒中肢体痉挛90例临床观察[J].江苏中医药,2017,49(1):53-55.
[10]谢莉红,陈军,蒋珍秀,等.脑卒中患者双上肢F波的变化及其评价肢体痉挛的可靠性[J].中国神经免疫学和神经病学杂志,2016,23(5):322-326.
[11]田梦,娄天伟,冷军.手针联合电针治疗脑卒中手痉挛患者的临床疗效观察[J].中国针灸,2017,37(9):932-935.
[12]Doha D,Buskila Y,Ooi L.Impairments in motor neurons,interneurons and astrocytes contribute to hyperexcitability in ALS:underlying mechanisms and paths to therapy[J].Molecular Neurobiology,2017,6(3):1-9.
[13]古春青,武继涛.化瘀通脉汤联合针刺对脑卒中后肢体痉挛患者血清NSE、Hcy水平的影响[J].中医学报,2017,9(12):2549-2552.
[14]Cabaj AM,Majczyński H,Couto E,et al.Serotonin controls initiation of locomotion and afferent modulation of coordination via 5-HT7 receptors in adult rats[J].Journal of Physiology,2016,595(1):17-22.
[15]单衍丽,刘敬花.刺络放血合电针治疗脑卒中上肢痉挛30例[J].中国针灸,2017,37(3):301-302.
[16]艾克拜尔·哈里克,逯霞,许健,等.选择性脊神经后根部分切断术治疗下肢痉挛性瘫痪的疗效及血清NSE、转化生长因子β1浓度的变换[J].中国现代手术学杂志,2017,21(2):149-152.