培养基配比对‘金踯躅’茎段组培和试管苗生根的影响
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摘要
<正>‘金踯躅’(Rhododendron molle ‘Jinzhizhu’)是园林绿化植物中不可多得的黄色杜鹃花品种,由羊踯躅(Rhododendron molle G. Don)实生苗开花植株经芽变选育获得,‘金踯躅’的花冠金黄色,与羊踯躅的纯黄色花冠有明显区别。该品种无种子繁殖体,并且扦插繁殖困难。目前,有关羊踯躅的繁育研究主要集中于繁殖生物学以及扦插和压条方法等方面~([1],[2]1-4,[3]3-16,[4]),但对羊踯躅组培快繁的研
Taking stem segments of one-year-old branch of Rhododendron molle ‘Jinzhizhu' as explants, and using WPM medium(containing 6.5 g·L~(-1) agar, pH 5.6) as basic medium, effects of medium proportion on differentiation of stem segment, proliferation of adventitious bud and rooting of plantlet were studied. The results show that differentiation rate of stem segment is the highest(83.3%) in induction medium(containing 6.5 g·L~(-1) agar and 30 g·L~(-1) sucrose, pH 5.6) with 1.0 mg·L~(-1 )2-ip and 0.1 mg·L~(-1 )NAA, and is significantly higher than that in other media; proliferation rate of adventitious bud is the highest(93.3%) in proliferation medium(containing 6.5 g·L~(-1) agar, pH 5.6) with 1.0 mg·L~(-1 )2-ip, 0.05 mg·L~(-1 )NAA and 30 g·L~(-1) sucrose or 1.5 mg·L~(-1 )2-ip, 0.10 mg·L~(-1 )NAA and 20 g·L~(-1) sucrose, and proliferation multiple is also bigger. Rooting rate of plantlet is the highest in rooting medium(containing 6.5 g·L~(-1) agar and 30 g·L~(-1) sucrose, pH 5.6) with 0.50 mg·L~(-1) NAA, which reaches 70.0%, but combined addition of NAA and IBA has no evident promotion effect on rooting of R. molle ‘Jinzhizhu' plantlet.
Taking stem segments of one-year-old branch of Rhododendron molle ‘Jinzhizhu' as explants, and using WPM medium(containing 6.5 g·L~(-1) agar, pH 5.6) as basic medium, effects of medium proportion on differentiation of stem segment, proliferation of adventitious bud and rooting of plantlet were studied. The results show that differentiation rate of stem segment is the highest(83.3%) in induction medium(containing 6.5 g·L~(-1) agar and 30 g·L~(-1) sucrose, pH 5.6) with 1.0 mg·L~(-1 )2-ip and 0.1 mg·L~(-1 )NAA, and is significantly higher than that in other media; proliferation rate of adventitious bud is the highest(93.3%) in proliferation medium(containing 6.5 g·L~(-1) agar, pH 5.6) with 1.0 mg·L~(-1 )2-ip, 0.05 mg·L~(-1 )NAA and 30 g·L~(-1) sucrose or 1.5 mg·L~(-1 )2-ip, 0.10 mg·L~(-1 )NAA and 20 g·L~(-1) sucrose, and proliferation multiple is also bigger. Rooting rate of plantlet is the highest in rooting medium(containing 6.5 g·L~(-1) agar and 30 g·L~(-1) sucrose, pH 5.6) with 0.50 mg·L~(-1) NAA, which reaches 70.0%, but combined addition of NAA and IBA has no evident promotion effect on rooting of R. molle ‘Jinzhizhu' plantlet.
引文
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[14] 李国树,李文春,徐成东,等.几种植物生长调节剂对山茶花扦插生根的影响[J].北方园艺,2011(14):65-69.
[2] 翁明武.羊踯躅压条繁殖和组织培养技术研究[D].贵阳:贵州师范大学生命科学学院,2008.
[3] 石登红.羊踯躅种子萌发与扦插繁殖技术研究[D].贵阳:贵州师范大学生命科学学院,2006.
[4] 杨灿娇,郑硕理,杨荣萍,等.羊踯躅和日本杜鹃的传粉生物学与杂交育种初探[J].中国野生植物资源,2016,35(6):47-52.
[5] 林魁,魏云华,魏宾斌.高山杜鹃组培快繁研究进展[J].现代农业科技,2017(13):144-145.
[6] 毛元荣,周根余.杜鹃组织培养研究进展[J].株洲师范高等专科学校学报,2003,8(5):20-24.
[7] 蓝伟根,白宇清,谢利娟.杜鹃组织培养研究进展[J].南方林业科学,2016,44(4):25-28.
[8] 苗永美.几种杜鹃组织培养技术研究[D].成都:四川农业大学林学院,2004:2-5,31-36.
[9] 郭颖.三种高山杜鹃组织培养快繁技术研究[D].北京:北京林业大学园林学院,2015.
[10] 周艳,陈训.马缨杜鹃继代培养培养基配方研究[J].安徽农业科学,2007,35(29):9213-9214.
[11] 陈平芬,王连润,高飞,等.常绿杂交杜鹃组培快繁研究[J].安徽农业科学,2012,40(36):17482-17484.
[12] 顾宏辉,袁群英,朱春燕,等.羊踯躅的组织培养与快速繁殖[J].植物生理学通讯,2006,42(4):683.
[13] 苗永美,简兴.杜鹃组培的研究[J].北方园艺,2004(3):76-77.
[14] 李国树,李文春,徐成东,等.几种植物生长调节剂对山茶花扦插生根的影响[J].北方园艺,2011(14):65-69.