miR-31在COPD患者外周血中的表达及其临床意义
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摘要
目的检测慢性阻塞性肺疾病(COPD)患者外周血单个核细胞(PBMCs)中微小RNA-31(miR-31)表达量并探讨其临床意义。方法采用实时荧光定量聚合酶链反应(qPCR)的方法检测38例COPD患者(COPD组)、15例急性支气管炎(AB)患者(AB组)和40例体检健康者(对照组)PBMCs中miR-31的表达,并对15例治疗后患者进行跟踪检测。酶联免疫吸附测定法(ELISA)检测COPD患者血浆中白细胞介素4(IL-4)和γ-干扰素(IFN-γ)水平。结果 COPD患者PBMC中miR-31的表达明显高于对照组和AB组,AB组PBMC中miR-31的表达明显高于对照组;COPD患者治疗后好转后miR-31的表达明显下降;COPD患者临床症状严重者其PBMC中miR-31的表达更高,COPD患者PBMC中miR-31的表达与血浆CRP水平呈负相关(r=-0.663 8,P<0.05),COPD患者PBMC中miR-31的表达与血浆中IL-4水平呈负相关(r=-0.476 4,P<0.05)。结论 COPD患者PBMC中miR-31的表达参与COPD的发生与发展,其可能在Th2类细胞中起重要作用。
Objective To investigate the expression of miR-31 in peripheral blood mononuclear cells(PBMCs)of patients with chronic obstructive pulmonary disease(COPD)and explore its clinical significance.Methods The expression of miR-31 in PBMCs from 38 patients with COPD(COPD group),15 patients with acute bronchitis(AB group),40 Healthy persons(control group)and 15 patients treated after tracking were measured by qPCR.The plasma level of IL-4 and IFN-γin patients with COPD were measured by enzyme linked immunosorbent assay.Results The expression of miR-31 in PBMC of patients with COPD was higher than AB group and control group,the expression of miR-31 in PBMC of patients with AB group were higher than control group,and it was decreased after treatment in patients with COPD;The expression of miR-31 in serious patients with COPD was higher than the other patients,the expression of miR-31 in patients with COPD was negative correlated with CRP level(r=-0.663 8,P<0.05).The expression of miR-31 in patients with COPD was negative correlated with the level of IL-4 in plasma(r=-0.476 4,P<0.05).Conclusion The expression of miR-31 in patients with COPD is significantly increased,which is related with the occurrence and development of COPD,and it is probably to impact the function of Th2 type cells.
Objective To investigate the expression of miR-31 in peripheral blood mononuclear cells(PBMCs)of patients with chronic obstructive pulmonary disease(COPD)and explore its clinical significance.Methods The expression of miR-31 in PBMCs from 38 patients with COPD(COPD group),15 patients with acute bronchitis(AB group),40 Healthy persons(control group)and 15 patients treated after tracking were measured by qPCR.The plasma level of IL-4 and IFN-γin patients with COPD were measured by enzyme linked immunosorbent assay.Results The expression of miR-31 in PBMC of patients with COPD was higher than AB group and control group,the expression of miR-31 in PBMC of patients with AB group were higher than control group,and it was decreased after treatment in patients with COPD;The expression of miR-31 in serious patients with COPD was higher than the other patients,the expression of miR-31 in patients with COPD was negative correlated with CRP level(r=-0.663 8,P<0.05).The expression of miR-31 in patients with COPD was negative correlated with the level of IL-4 in plasma(r=-0.476 4,P<0.05).Conclusion The expression of miR-31 in patients with COPD is significantly increased,which is related with the occurrence and development of COPD,and it is probably to impact the function of Th2 type cells.
引文
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[25]LUAN L,SHI J,YU Z,et al.The major miR-31target genes STK40and LATS2and their implications in the regulation of keratinocyte growth and hair differentiation[J].Exper Dermatol,2017,26(6):497-504.
[26]WU D,WANG B,SHANG J,et al.miR-31reduces cell growth of papillary thyroid carcinoma by RNA-binding protein HUR[J].Clin Labor,2015,61(11):1625-1634.
[2]SOLER J J,MARTINEZ M A,ROMAN P,et al.Severe acute exacerbations and mortality in patients with chronic obstructive pulmonary disease[J].Thorax,2005,60(11):925-931.
[3]SOLER N,EWIG S,TORRES A,et al.Airway inflammation and bronchial microbial patterns in patients with stable chronic obstructive pulmonary disease[J].Europ Respir J,1999,14(5):1015-1022.
[4]YANG S,XIE N,CUI H,et al.miR-31is a negative regulator of fibrogenesis and pulmonary fibrosis[J].Faseb J,2012,26(9):3790-3799.
[5]MOFFETT H F,CARTWRIGHT N R,KIM H J,et al.The microRNA miR-31inhibits CD8+T cell function in chronic viral infection[J].Nat Immunol,2017,18(7):791-799.
[6]MACNEE W.Pulmonary and systemic oxidant/antioxidant imbalance in chronic obstructive pulmonary disease[J].Proc Amer Thorac Soc,2005,2(1):50-60.
[7]KANG M J,LEE C G,LEE J Y,et al.Cigarette smoke selectively enhances viral PAMP-and virus-induced pulmonary innate immune and remodeling responses in mice[J].J Clin Invest,2008,118(8):2771-2784.
[8]MILARA J,JUAN G,PEIRO T,et al.Neutrophil activation in severe,early-onset COPD patients versus healthy non-smoker subjects in vitro:Effects of antioxidant therapy[J].Respiration,2012,83(2):147-158.
[9]FORSSLUND H,YANG M,MIKKO M,et al.Gender differences in the T-cell profiles of the airways in copd patients associated with clinical phenotypes[J].InterJChro Dis,2017,12(12):35-48.
[10]DEMEO D L,CAREY V J,CHAPMAN H A,et al.Familial aggregation of FEF(25-75)and FEF(25-75)/FVCin families with severe,early onset COPD[J].Thorax,2004,59(5):396-400.
[11]WANG M,HUANG Y,LIANG Z,et al.Plasma miRNAs might be promising biomarkers of chronic obstructive pulmonary disease[J].Clin Respir J,2016,10(1):104-111.
[12]LACEDONIA D,PALLADINO G P,FOSCHINO-BAR-BARO M P,et al.Expression profiling of miRNA-145and miRNA-338in serum and sputum of patients with copd,asthma,and asthma-copd overlap syndrome phenotype[J].Inter J Chro Dis,2017,12(7):1811-1817.
[13]MA Y,CHEN Y,LIN J,et al.Circulating miR-31as an effective biomarker for detection and prognosis of human cancer:A meta-analysis[J].Oncotarget,2017,8(17):28660-28671.
[14]YU M,LIANG H,FU Z,et al.BAP1suppresses lung cancer progression and is inhibited by mir-31[J].Oncotarget,2016,7(12):13742-13753.
[15]OKUDELA K,TATEISHI Y,UMEDA S,et al.Allelic imbalance in the miR-31host gene locus in lung cancer-its potential role in carcinogenesis[J].PLoS One,2014,9(6):e100581.
[16]VALASTYAN S,REINHARDT F,BENAICH N,et al.A pleiotropically acting microRNA,miR-31,inhibits breast cancer metastasis[J].Cell,2009,137(6):1032-1046.
[17]LV C,LI F,LI X,et al.miR-31promotes mammary stem cell expansion and breast tumorigenesis by suppressing wnt signaling antagonists[J].Nature Com,2017,8(1):1036.
[18]MALARDO T,GARDINASSI L G,MO-REIRA B P,et al.MicroRNA expression signatures in lungs of mice infected with mycobacterium tuberculosis[J].Tuberculosis,2016,101(12):151-159.
[19]ZHOU M,YU G,YANG X,et al.Circulating microRNAs as biomarkers for the early diagnosis of childhood tuberculosis infection[J].Mole Med Rep,2016,13(6):4620-4626.
[20]BHELA S,ROUSE B T.miR-31:A key player in CD8T-cell exhaustion[J].Cell Mole Immunol,2017,14(12):954-956.
[21]BORSKA L,ANDRYS C,CHMELAROVA M,et al.Roles of miR-31and endothelin-1in psoriasis vulgaris:Pathophysiological functions and potential biomarkers[J].Physiol Res,2017,66(6):987-992.
[22]范薇,唐元家,曲波,等.系统性红斑狼疮患者外周血细胞miR-31的表达[J].上海交通大学学报(医学版),2011,31(1):39-42.
[23]赵静,陈光辉,刘德军,等.原发性肺癌患者外周血中microRNA-31的表达及意义[J].郑州大学学报(医学版),2014,49(5):684-686.
[24]VAN DER HEIDE V,MOHNLE P,RINK J,et al.Down-regulation of microRNA-31in CD4+T cells contributes to immunosuppression in human sepsis by promoting TH2skewing[J].Anesthesiol,2016,124(4):908-922.
[25]LUAN L,SHI J,YU Z,et al.The major miR-31target genes STK40and LATS2and their implications in the regulation of keratinocyte growth and hair differentiation[J].Exper Dermatol,2017,26(6):497-504.
[26]WU D,WANG B,SHANG J,et al.miR-31reduces cell growth of papillary thyroid carcinoma by RNA-binding protein HUR[J].Clin Labor,2015,61(11):1625-1634.