维药溃结安对异常黏液质溃疡性结肠炎大鼠干预效果的血清代谢组学研究
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摘要
目的:利用核磁共振测定技术探讨维药溃结安对异常黏液质溃疡性结肠炎(UC)模型大鼠的干预效果,为UC的个体化治疗提供实验依据。方法:将Wistar大鼠随机分为UC溃结安干预(A)组和UC病症溃结安干预(B)组。其中,A组运用DNCB复合乙酸法建立UC模型大鼠,在此基础上用溃结安干预21d。B组以湿寒饲料(芫荽实和菠菜实),在温度(6±1)℃,相对湿度85%-95%的环境中进行喂养,建立异常黏液质证载体模型大鼠后运用DNCB复合乙酸法建立异常黏液质UC模型大鼠,在此基础上用溃结安干预21d。对两组模型大鼠进行鉴定,并收集血清,进行核磁共振氢谱(1H-NMR)测定,运用正交偏最小二乘判别(OPLS-DA)分析所采集的图谱,确认差异性代谢组分。结果:与A组比较,B组大鼠血清中乳酸、丙氨酸含量降低,β-羟丁酸、丙酮酸、乙酰乙酸含量升高(P<0.05)。结论:维药溃结安对异常黏液质UC大鼠的干预效果优于对UC大鼠的干预效果。
Objective:To investigate the effects of Kuijie'an on rat model of ulcerative colitis(UC) with abnormal balgam carrier by nuclear magnetic resonance(NMR) technology,in order to provide experimental evidence for the individualized treatment of UC.Methods:Male Wistar rats were randomly divided into UC Kui Jie'an treatment group(group A) and abnormal balgam carrier UC Kui Jie'an treatment group(Group B).The rats in the Group A suffered the DNCB andacetic acid stress to stablish the rat model of UC.Then,the Kui Jie'an was used to treat the model for 21 days.The rats in the Group B were fed with wet and cold diet(seeds of spinach and Parsley) in a cold(6±1)℃ and humid(85%-95%) atmosphere to establish the abnormal balgam carrier model,which then suffered the DNCB andacetic acid stress.Then,the Kui Jie'an was used to treat the model for21 days.The serum samples were collected to conduct the ~1H-NMR analysis.Then,the data was analyzed by orthogonal partial squares-discriminant analysis(OPLS-DA) software to identify discrepant metabolic components.Results:Compared with Group A,the levels of lactic acid,alanine in the Group B were reduced,while the levels of β-hydroxybutyric acid,pyroracemic acid and acetacetic acid were increased(P<0.05).Conclusion:The effects of Uyghur medicine Kui Jie'an on rat model of UC with abnormal balgam are superior to the effects on the rat model of UC.
Objective:To investigate the effects of Kuijie'an on rat model of ulcerative colitis(UC) with abnormal balgam carrier by nuclear magnetic resonance(NMR) technology,in order to provide experimental evidence for the individualized treatment of UC.Methods:Male Wistar rats were randomly divided into UC Kui Jie'an treatment group(group A) and abnormal balgam carrier UC Kui Jie'an treatment group(Group B).The rats in the Group A suffered the DNCB andacetic acid stress to stablish the rat model of UC.Then,the Kui Jie'an was used to treat the model for 21 days.The rats in the Group B were fed with wet and cold diet(seeds of spinach and Parsley) in a cold(6±1)℃ and humid(85%-95%) atmosphere to establish the abnormal balgam carrier model,which then suffered the DNCB andacetic acid stress.Then,the Kui Jie'an was used to treat the model for21 days.The serum samples were collected to conduct the ~1H-NMR analysis.Then,the data was analyzed by orthogonal partial squares-discriminant analysis(OPLS-DA) software to identify discrepant metabolic components.Results:Compared with Group A,the levels of lactic acid,alanine in the Group B were reduced,while the levels of β-hydroxybutyric acid,pyroracemic acid and acetacetic acid were increased(P<0.05).Conclusion:The effects of Uyghur medicine Kui Jie'an on rat model of UC with abnormal balgam are superior to the effects on the rat model of UC.
引文
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[15]古丽尼沙·莫沙,阿吉古丽·努尔买买提,张景萍,等.维吾尔医学异常黏液质证载体模型大鼠血清代谢组学研究.中南大学学报(医学版),2014,40(1):18-24
[16]麦日排提·阿卜杜拉,阿吉古丽·努尔买买提,古丽尼沙·莫沙,等.异常黑胆质型UC病证大鼠血清的核磁共振代谢组学.世界华人消化杂志,2014,22(35):5414-5421
[17]Qi S W,Tu Z G,Peng W J,et al.1HNMR-based serum metabolic profiling in compensated and decompensated cirrhosis.World J Gastroenterol,2012,18(3):285-290
[18]Zhang Y,Lin L,Xu Y,et al.1HNMR-based spectroscopy detects metabolic alterations in serum of patients with early-stage ulcerative colitis.Biochem Biophys Res Commun,2013,433(4):547-551
[19]阿衣古丽·玉努斯,库热西·玉努斯.基于异常黑胆质证载体大鼠模型尿液代谢组学研究.新疆医科大学学报,2010,35(6):733-739
[20]阿吉古丽·努尔买买提,古丽尼沙·莫沙,黄静静,等.异常黏液质证大鼠模型与内分泌-免疫功能的关系.中华中医药杂志,2014,30(4):1031-1034
[2]Kazuo Azuma,Tomohiro Osaki,Takashi Wakuda,et al.Beneficial and preventive effect of chitin nanofibrils in a dextran sulfate sodium-induced acute ulcerative colitis model.Carbohydrate Polymers:Scientific and Technological Aspects of Industrially Important Polysaccharides,2012,87(2):1399-1403
[3]欧阳钦,潘国宗,温忠慧,等.炎症性肠病诊断治疗规范的建议.胃肠病学,2001,6(1):56-59
[4]Hanauer S B.Inflammatory bowel disease:epidemiology,pathogenesis,and therapeutic opportunities.Inflam Bowel Disease,2006,12(Suppl 1):3-9
[5]Thia K T,Lotus E V,Sandbom W J,et al.An update on the epidemiology of inflammatory bowel disease in Asia.Am J Gastroenterol,2008,10(3):3167-3182
[6]哈木拉提·吾甫尔.维吾尔医气质、体液论及其现代研究.乌鲁木齐:新疆科学技术出版社,2003:44-52
[7]吐尔洪·吾买尔.维吾尔基础理论学.乌鲁木齐:新疆人民卫生出版社,2004:1-6
[8]雅森·米吉提,库热西·玉努斯.维吾尔医学体液辨证分型及其相关疾病.新疆医科大学学报,2010,33(6):612-613
[9]Tang H R,Wang Y L.Metabonomics:a revolution in progress.Prog Biochem Biophys,2006,33(5):401-417
[10]巴吐尔·买买提明,阿仙姑·哈斯木.标准化方法对大鼠尿液1H磁共振谱代谢物分析结果的影响.中南大学学报(医学版),2010,35(12):1214-1218
[11]Geir Aamodt,May-Bente Bengtson,Morten H Vatn,et al.Can temperature explain the latitudinal gradient of ulcerative colitis Cohort of Norway Aamodt.BMC Public Health,2013,13(530):2-8
[12]Yin Shi,Shuang Li,Huangan Wu,et al.Interventional effect of electro acupuncture combined with medicine on monoamine neurotransmitters in hypothalamus of rats with ulcerative colitis.Neural Regen Res,2007,2(9):536-539
[13]Kellett J.Extinction deficit and fear reinstatement after elect rical stimulation of the amydale:implications for kindling associated fear and anxiety.Neuroscience,2004,127(2):277-287
[14]阿地力江·伊明,潘建春,哈木拉提·吾甫尔,等.几种不同方法建立维医异常黏液质阳痿病证模型的研究.新疆医科大学学报,2010,33(11):1276-1277
[15]古丽尼沙·莫沙,阿吉古丽·努尔买买提,张景萍,等.维吾尔医学异常黏液质证载体模型大鼠血清代谢组学研究.中南大学学报(医学版),2014,40(1):18-24
[16]麦日排提·阿卜杜拉,阿吉古丽·努尔买买提,古丽尼沙·莫沙,等.异常黑胆质型UC病证大鼠血清的核磁共振代谢组学.世界华人消化杂志,2014,22(35):5414-5421
[17]Qi S W,Tu Z G,Peng W J,et al.1HNMR-based serum metabolic profiling in compensated and decompensated cirrhosis.World J Gastroenterol,2012,18(3):285-290
[18]Zhang Y,Lin L,Xu Y,et al.1HNMR-based spectroscopy detects metabolic alterations in serum of patients with early-stage ulcerative colitis.Biochem Biophys Res Commun,2013,433(4):547-551
[19]阿衣古丽·玉努斯,库热西·玉努斯.基于异常黑胆质证载体大鼠模型尿液代谢组学研究.新疆医科大学学报,2010,35(6):733-739
[20]阿吉古丽·努尔买买提,古丽尼沙·莫沙,黄静静,等.异常黏液质证大鼠模型与内分泌-免疫功能的关系.中华中医药杂志,2014,30(4):1031-1034