阿胶类保健食品中阿胶成分和牛皮源成分检测及质量评价
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摘要
采用超高效液相色谱-串联质谱法对6种类型、共计67批阿胶类保健食品中阿胶成分和牛皮源成分同时进行测定,结合阿胶含量、水分、蛋白质含量的测定结果对每种剂型中阿胶成分和牛皮源成分进行分析与评价,对日后专项标准制定和监管方向提出建议。结果表明:阿胶片中阿胶含量较高,为39%～65%,蛋白质含量也较高,为68%～84%,牛皮源成分的检出率为22. 8%,检出且超过标准规定的有2批;阿胶粉、阿胶胶囊、阿胶含片、阿胶核桃糕和阿胶口服液中,以4种氨基酸代表的阿胶含量差异较大,与阿胶片相比数值较低,检出牛皮源成分的有16批,但均未超过标准规定。超高效液相色谱-串联质谱法可以同时快速、准确地对阿胶成分特征峰和牛皮源成分特征峰进行判别;加强阿胶类保健食品的原材料监管、并按类型区别制定阿胶类保健食品中牛皮源掺入限值标准十分必要。
Donkey-hide gelatin and bovine-hide gelatin were determined in 6 kinds of formulations,such as powder,capsule,lozenge,jell,and oral solution,and 67 samples of donkey-hide gelatin using UPLC-MS/MS. The contents of donkey-hide gelatin and bovine-hide gelatin were analysed and evaluated by measuring L-hydroxyproline,glycine,alanine,L-proline,moisture,and protein. The content of donkey-hide gelatin in tablets was found to be 39%-65%,and protein was 68%-84%. The detection rate of bovine-hide gelatin was 22. 8%,and 2 batches exceeded the standard. The content of donkey-hide gelatin indicated as the sum of L-hydroxyproline,glycine,alanine,and Lproline had significant differences in other 5 formulations,as they were all lower than that in tablets. Moreover,16 batches were detected to contain bovine-hide gelatin,but none of them exceeded the standard. The present method is repeatable and sensitive to distinguish donkey-hide gelatin and bovine-hide gelatin. It is necessary to pay attention to raw materials of donkey-hide gelatin and formulate standards for different formulations.
Donkey-hide gelatin and bovine-hide gelatin were determined in 6 kinds of formulations,such as powder,capsule,lozenge,jell,and oral solution,and 67 samples of donkey-hide gelatin using UPLC-MS/MS. The contents of donkey-hide gelatin and bovine-hide gelatin were analysed and evaluated by measuring L-hydroxyproline,glycine,alanine,L-proline,moisture,and protein. The content of donkey-hide gelatin in tablets was found to be 39%-65%,and protein was 68%-84%. The detection rate of bovine-hide gelatin was 22. 8%,and 2 batches exceeded the standard. The content of donkey-hide gelatin indicated as the sum of L-hydroxyproline,glycine,alanine,and Lproline had significant differences in other 5 formulations,as they were all lower than that in tablets. Moreover,16 batches were detected to contain bovine-hide gelatin,but none of them exceeded the standard. The present method is repeatable and sensitive to distinguish donkey-hide gelatin and bovine-hide gelatin. It is necessary to pay attention to raw materials of donkey-hide gelatin and formulate standards for different formulations.
引文
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[3]赵曦,翟乙娟,都恒青,等. 30种商品阿胶的质量比较[J].中国药学杂志,2000,35(10):690-692.
[4]高景会,王蕊,范锋.阿胶现代研究进展[J].中国药事,2011,25(4):396-401.
[5]尚汝瑶,杨昭鹏,冯磊.国家药品补充检验方法管理工作的现状与思考[J].中国药事,2014,28(6):576-579.
[6]陈慧慧,冯明建,朱海芳,等.阿胶药理研究进展[J].药物研究,2014,31(1):23-26.
[7]王若光,尤昭玲,刘小丽,等.基于激光解析/离子化-飞行时间质谱技术的中药阿胶蛋白质组分析[J].中国组织工程研究与临床康复,2007,13:2 518-2 521.
[8]吴亚君,王斌,刘鸣畅,等.阿胶中马和驴成分的实时荧光PCR检测[J].食品科学,2014,35(8):85-88.
[9]张贵锋,刘涛,王前,等.中药阿胶的质量控制方法研究[J].药物生物技术,2009,3:250-254.
[10]国家药典委员会.中华人民共和国药典[M].一部.北京:化学工业出版社,2015:189-190.
[11]中国食品药品检定研究院.阿胶中牛皮源质量的补充检验方法[EB/OL].[2014-12-21]. http://www.doc88. com/p-3137587400245. html.
[12]国家药典委员会.中华人民共和国药典[M].四部.北京:化学工业出版社,2015:103-104.
[13] GB 5009. 5—2010,食品安全国家标准食品中蛋白质的测定[S].北京:中华人民共和国卫生部,2010.
[14]毛跟年,郭倩,瞿建波,等.阿胶化学成分及药理作用研究进展[J].动物医学进展,2010,31(11):83-85.
[15]汪学德,崔英德,刘兵戈,等.芝麻各成分相关性分析[J].中国油脂,2015,40(11):99-103.
[16]陆俊,赵安琪,成策,等.核桃营养成分与生理活性及开发利用[J].食品与机械,2014,30(6):238-242.
[17]王蓉珍,赵子青,林勤保,等.大枣功效成分检测的研究进展[J].食品工业科技,2012,33(4):423-426.