油茶果壳多酚对酪氨酸酶活性的影响
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摘要
研究了油茶果壳的提取物油茶多酚对酪氨酸酶活性的影响,考察了不同底物浓度下,油茶多酚对酪氨酸酶活性的影响。结果表明,反应时间、底物浓度对酪氨酸酶的活性有不同程度的影响,油茶多酚对酪氨酸酶具有激活作用,在25 min时,油茶多酚对酪氨酸激活作用的最佳条件为底物浓度为7.5×10~(-5) mmol/L、油茶多酚浓度为2 000μg/mL。
The effect of camellia oleifera extract on tyrosinase activity was studied. The effects of different reaction time and substrate concentration on tyrosinase activity of camellia oleifera polyphenols were investigated. The results showed that the reaction time, substrate concentration had obvious influence on the activity of tyrosinase. Camellia oleifera polyphenols had activation effect on tyrosinase. The optimum conditions for the activation of tyrosine were as follows: At 25 min, the concentration of the substrate was 7.5×10~(-5) mmol/L and the concentration of camellia oleifera polyphenols was 2 000 μg/mL.
The effect of camellia oleifera extract on tyrosinase activity was studied. The effects of different reaction time and substrate concentration on tyrosinase activity of camellia oleifera polyphenols were investigated. The results showed that the reaction time, substrate concentration had obvious influence on the activity of tyrosinase. Camellia oleifera polyphenols had activation effect on tyrosinase. The optimum conditions for the activation of tyrosine were as follows: At 25 min, the concentration of the substrate was 7.5×10~(-5) mmol/L and the concentration of camellia oleifera polyphenols was 2 000 μg/mL.
引文
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[4]卢玉栋,柯金炼,林炎伟,等.载银油茶果壳抗菌剂的制备和性能[J].广州化学,2017,42(3):55-58.
[5]周建斌,张齐生.油茶壳制活性炭的研究[J].林业工程学报,2003,17(5):54-55.
[6]Guo H,Bi C,Zeng C,et al.Camellia oleifera seed shell carbon as an efficient renewable bio-adsorbent for the adsorption removal of hexavalent chromium and methylene blue from aqueous solution[J].Journal of Molecular Liquids,2017(249):629-636.
[7]Gao J J,Qin Y B,Zhou T,et al.Adsorption of methylene blue onto activated carbon produced from tea(Camellia sinensis,L.)seed shells:Kinetics,equilibrium and thermodynamics studies[J].Journal of Zhejiang University.Science.B,2013,14(7):650-658.
[8]吴开金,陈涵,林冠烽.油茶壳活性炭的制备工艺研究[J].福建林业科技,2011,38(2):92-94.
[9]Lu Y D,Wu W L,Lin S,et al.Removal of Cr(Ⅵ)from aqueous solution using camellia oleifera abel shells[J].Materials Science Forum,2013,744:463-468.
[10]杨小敏,刘建平,李倩倩,等.响应面法优化超声辅助提取油茶壳中单宁的工艺[J].食品工业科技,2012,33(9):215-217.
[11]沈建福,姜天甲,王徐卿.油茶壳中总黄酮的最佳提取工艺研究[J].中国粮油学报,2008,23(3):104-106.
[12]寇巧花,吴雪辉,李丽,等.油茶籽壳粗提物的抗氧化活性研究[J].食品研究与开发,2010,31(3):73-76.
[13]郭会琴,熊晶晶,颜流水,等.高效液相色谱-质谱法测定油茶果壳中5种多酚类化合物的含量[J].理化检验-化学分册,2015,51(11):1493-1496.
[14]刘杰超,焦中高,张春岭,等.苹果多酚提取物对酪氨酸酶的抑制作用[J].日用化学工业,2013,43(06):414-417,456.
[15]段玉清,刘睿,谢笔钧.莲花原花青素对酪氨酸酶活力和黑色素生物合成影响的初步研究[J].食品科学,2004,25(3):169-174.
[16]雷铁池,朱文元,夏明玉,等.89味中药乙醇提取物对酪氨酸酶活性的上调作用[J].临床皮肤科杂志,1999,28(3):147-149.