藏鸡禽流感病毒抗体消长规律研究
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摘要
为探讨鸡禽流感疫苗的免疫效果,总结禽流感病毒抗体在藏鸡体内的消长规律,制定最佳的鸡场禽流感免疫程序。对林芝市西藏农牧学院临床兽医楼养殖的86只藏鸡进行禽流感疫苗的免疫接种,并按程序完成3次免疫后每隔2周随机对接种过禽流感疫苗的20只藏鸡进行抗体检测,检测直至完成最后一次免疫后第28周,用酶联免疫吸附试验(ELISA)检测抗体数值,用血凝(HA)和血凝抑制(HI)试验检测抗体效价。血凝抑制实验表明,31 d龄藏鸡完成3次禽流感疫苗免疫,经2周后,抗体水平迅速上升,第10周抗体水平达到高水平并维持至第20周,直到第24周,抗体水平降至临界值4 log2附近;ELISA试验表明,31 d龄藏鸡完成3次禽流感疫苗免疫,经2周后,抗体水平迅速上升,第10～20周,抗体水平达到高峰,持续保持在一定高水平。18周开始,抗体水平开始逐渐下降,直至第28周,抗体水平降至临界值50%附近。31 d龄藏鸡完成3次禽流感疫苗免疫第4～24周,血凝抑制实验结果表明检测所有藏鸡血清中抗体效价平均值都在标准值4 log2以上,抽检合格率均大于标准值70%;ELISA试验结果表明抗体数值(PI)均在标准值50%以上。实验结果表明按本防疫程序防疫禽流感后,有效抗体在藏鸡体内维持时间为5个半自然月,建议第24周进行下一次免疫。这为制定西藏林芝地区藏鸡禽流感最佳免疫疫程序提供有效的参考依据。
In order to explore the immune effect of chicken avian influenza vaccine, summarize the growth and decline of avian influenza virus antibodies in Tibetan chickens, and develop the best chicken farm avian influenza immunization program. Avian influenza vaccine was vaccinated against 86 Tibetan chickens cultured in the clinical veterinary building of Tibet Agriculture and Animal Husbandry College of Nyingchi City, and 20 Tibetan chickens vaccinated with the bird flu vaccine were randomly selected every 2 weeks after completing three immunizations according to the procedure. Antibody detection was performed until the 28 th week after the completion of the last immunization, and the antibody value was detected by an enzyme-linked immunosorbent assay(ELISA), and the antibody titer was detected by hemagglutination(HA) and hemagglutination inhibition(HI) assays. Hemagglutination inhibition experiments showed that 31-day-old Tibetan chickens completed three avian influenza vaccine immunizations. After 2 weeks, the antibody levels increased rapidly. At the 10 th week, the antibody levels reached a high level and remained until the 14 th week, starting at 14 weeks. Antibody levels began to decline slowly until the 24 th week, the antibody level fell to the critical value of 4 log2; ELISA test showed that 31 days old Tibetan chickens completed three avian influenza vaccine immunizations, after 2 weeks, the antibody levels rose rapidly, the 10 th week～ At week 18, antibody levels peaked and remained at a high level. At the beginning of the 18 th week, antibody levels began to decline gradually, and by week 28, the antibody level fell to around 50% of the critical value. The 31-dayold Tibetan chicken completed three avian influenza vaccine immunizations from 4 to 24 weeks. The results of hemagglutination inhibition test showed that the average antibody titer in all Tibetan chicken serum was above the standard value of 4 log2, and the sampling pass rate was greater than the standard. The value was 70%; the ELISA test results showed that the antibody values(PI) were all above 50% of the standard value. The experimental results show that after the prevention of avian influenza according to the epidemic prevention program, the effective antibody is maintained in Tibetan chicken for 5 and a half natural months, and the next immunization is recommended at the 24 th week. This provides an effective reference for the development of the best immunization program for Tibetan avian influenza in Linzhi Prefecture, Tibet.
In order to explore the immune effect of chicken avian influenza vaccine, summarize the growth and decline of avian influenza virus antibodies in Tibetan chickens, and develop the best chicken farm avian influenza immunization program. Avian influenza vaccine was vaccinated against 86 Tibetan chickens cultured in the clinical veterinary building of Tibet Agriculture and Animal Husbandry College of Nyingchi City, and 20 Tibetan chickens vaccinated with the bird flu vaccine were randomly selected every 2 weeks after completing three immunizations according to the procedure. Antibody detection was performed until the 28 th week after the completion of the last immunization, and the antibody value was detected by an enzyme-linked immunosorbent assay(ELISA), and the antibody titer was detected by hemagglutination(HA) and hemagglutination inhibition(HI) assays. Hemagglutination inhibition experiments showed that 31-day-old Tibetan chickens completed three avian influenza vaccine immunizations. After 2 weeks, the antibody levels increased rapidly. At the 10 th week, the antibody levels reached a high level and remained until the 14 th week, starting at 14 weeks. Antibody levels began to decline slowly until the 24 th week, the antibody level fell to the critical value of 4 log2; ELISA test showed that 31 days old Tibetan chickens completed three avian influenza vaccine immunizations, after 2 weeks, the antibody levels rose rapidly, the 10 th week～ At week 18, antibody levels peaked and remained at a high level. At the beginning of the 18 th week, antibody levels began to decline gradually, and by week 28, the antibody level fell to around 50% of the critical value. The 31-dayold Tibetan chicken completed three avian influenza vaccine immunizations from 4 to 24 weeks. The results of hemagglutination inhibition test showed that the average antibody titer in all Tibetan chicken serum was above the standard value of 4 log2, and the sampling pass rate was greater than the standard. The value was 70%; the ELISA test results showed that the antibody values(PI) were all above 50% of the standard value. The experimental results show that after the prevention of avian influenza according to the epidemic prevention program, the effective antibody is maintained in Tibetan chicken for 5 and a half natural months, and the next immunization is recommended at the 24 th week. This provides an effective reference for the development of the best immunization program for Tibetan avian influenza in Linzhi Prefecture, Tibet.
引文
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[9]豆志明.禽流感的防控措施[J].养殖与饲料,2017(06):71-72.
[10]康华玲,王志斌.血凝与血凝抑制试验操作方法及注意事项[J].畜禽业,2016(06):40-41.
[2]胡祖禹,江家椿,韩行赟等.西藏高原藏鸡及白色来航鸡的一些高原生理特性研究[J].畜牧兽医学报,1984(01):27-31.
[3]黄西梅,李佳荣,骆荣彪等.商品肉鸡禽流感疫苗不同免疫程序免疫效果观察[J].中国畜牧兽医文摘,2017, 33(04):144-145.
[4]刘新,李金容,尚卫敏等.鸡禽流感的流行特点与防治措施[J].畜牧兽医科技信息,2019(01):139.
[5]王志坚,张宇.禽流感血凝和血凝抑制试验的影响因素分析[J].农民致富之友,2018(10):222.
[6]GB/T18936-2003.高致病性禽流感诊断技术[S].北京:中国标准出版社, 2003.
[7]陈莹娟.H5亚型高致病性禽流感免疫抗体检测结果研究[J].畜禽业,2017,28(12):15+18.
[8]肖连明,何月莲.浅析低致病性禽流感的特征及防治[J].农民致富之友,2017(22):229.
[9]豆志明.禽流感的防控措施[J].养殖与饲料,2017(06):71-72.
[10]康华玲,王志斌.血凝与血凝抑制试验操作方法及注意事项[J].畜禽业,2016(06):40-41.