HS-SPME-GC-MS分析百部及其蜜炙品挥发性成分
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摘要
目的:分析百部及其蜜炙品挥发性成分的差异,以期为百部化学成分的进一步开发利用提供参考依据。方法:采用顶空固相微萃取法(HS-SPME)萃取百部及其蜜炙品的挥发性成分,结合气质联用技术(GC-MS)进行分析测定,并应用色谱峰面积归一化法计算各成分的相对百分含量。结果:从生品及其蜜炙品共分离并鉴定出34个峰,主要为烯类,酚类和醛酮类;生品及其蜜炙品检测出14种共有成分,其中相对百分含量最高的成分均为茴香脑,分别占挥发性成分总量的40. 77%和37. 86%。结论:百部及其蜜炙品挥发性成分总离子流图的峰型相似,化学成分差异较大,本试验采用HS-SPME-GC-MS联用技术,比较分析百部及其蜜炙品挥发性成分,为百部化学成分的进一步开发利用提供科学参考依据。
Objective: To comparatively study the differences in the volatile components between stemona sessilifolia and its honey processed product to provide scientific basis for the further development and utilization of the chemical components of stemona sessilifolia. Methods: The volatile components of stemona sessilifolia and its honey processed product were extracted by using HS-SPME,the relative percentage of each constituent was analyzed by GC-MS,and the results were calculated by a chromatographic peak area normalization method. Results: A total of 70 constituents were detected out from the raw and honey processed product,and among them,35 constituents were identified which were mainly alkenes,phenols,aldehydes and ketones.Totally 14 common peaks were detected out from the raw and honey processed product,and anethole was with the highest concentration,which respectively accounted for 40. 77% and 37. 86% of the total volatile components. Conclusion: The volatile components of stemona sessilifolia and its honey processed product are similar in peak features of total ion flow diagrams,while quite different in their chemical compositions. HS-SPME-GC-MS is used to analyze the volatile components of stemona sessilifolia and its honey processed product,which provides scientific basis for the further development and utilization of the chemical components of stemona sessilifolia.
Objective: To comparatively study the differences in the volatile components between stemona sessilifolia and its honey processed product to provide scientific basis for the further development and utilization of the chemical components of stemona sessilifolia. Methods: The volatile components of stemona sessilifolia and its honey processed product were extracted by using HS-SPME,the relative percentage of each constituent was analyzed by GC-MS,and the results were calculated by a chromatographic peak area normalization method. Results: A total of 70 constituents were detected out from the raw and honey processed product,and among them,35 constituents were identified which were mainly alkenes,phenols,aldehydes and ketones.Totally 14 common peaks were detected out from the raw and honey processed product,and anethole was with the highest concentration,which respectively accounted for 40. 77% and 37. 86% of the total volatile components. Conclusion: The volatile components of stemona sessilifolia and its honey processed product are similar in peak features of total ion flow diagrams,while quite different in their chemical compositions. HS-SPME-GC-MS is used to analyze the volatile components of stemona sessilifolia and its honey processed product,which provides scientific basis for the further development and utilization of the chemical components of stemona sessilifolia.
引文
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2杨牧祥,方朝义,谷振勇,等.咳喘宁胶囊对慢性支气管炎大鼠血清、肺组织及支气管肺泡灌洗液SOD、CAT活性及MDA含量的影响[J].中国中医基础医学杂志,2002,8(1):14-18
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5 Umsumarng S,Pitchakarn P,Yodkeeree S,et al.Modulation of P-glycoprotein by Stemona alkaloids in human multidrug resistance leukemic cells and structural relationships[J].Phytomedicine,2017,34:182-190
6张囡.药典收载三种百部指纹图谱及止咳作用的比较研究[D].沈阳:沈阳药科大学硕士学位论文,2007
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9钟瑞敏,肖仔君,张振明,等.小茴香籽精油成分及其抗菌活性研究[J].林产化学与工业,2007。(6):36-40
10南京中医药大学.中药大辞典[M].2版.上海:上海科学技术出版社,2006:347-349
11刘宝双,张纪源.中小型鸡场鸡羽虱的防治[J].中国家禽,1990,(1):16
12张勤,王宗仪.皮下蝇蛆病及其防治[J].河北畜牧兽医,1998,14(1):36-37
13梁忠云,王国聪.草蒿脑资源及生物活性应用研究进展[J].广西林业科学,2010,39(1):49-51
14李红,刘芳.通过SO2和反-2-壬烯醛间的作用来提高啤酒风味稳定性[J].啤酒科技,2002,(9):10-11
15石向慧,纪禄风,伊琳,等.当归挥发油对自发性高血压大鼠肝脏脂代谢的影响[J].解放军药学学报,2017,33(2):113-119
16张金花,熊永爱.丹皮酚对大鼠缺血性心律失常及其心肌细胞miRNA-1表达的影响[J].中国实验方剂学杂志,2015,21(5):129-132
17周建中,王长峰,马勇,等.骨关节炎模型兔膝关节腔注射丹皮酚联合针刺足三里血清白细胞介素1β、肿瘤坏死因子α的变化[J].中国组织工程研究,2012,16(22):4096-4099
18董晓敏,刘布鸣,林霄,等.广西产香茅草挥发油的化学成分分析[J].广西科学,2009,16(3):302-304
2杨牧祥,方朝义,谷振勇,等.咳喘宁胶囊对慢性支气管炎大鼠血清、肺组织及支气管肺泡灌洗液SOD、CAT活性及MDA含量的影响[J].中国中医基础医学杂志,2002,8(1):14-18
3任建军,师光禄,谷继成,等.华北地区31种植物粗提物对朱砂叶螨Tetranychus cinnabarinus的触杀活性[J].北京农学院学报,2009,24(2):17-21
4陈晓霞,张晓丹,李红艳,等.基于哮喘小鼠模型研究生、制百部醇提物作用差异[J].中国中药杂志,2013,38(23):4084-4087
5 Umsumarng S,Pitchakarn P,Yodkeeree S,et al.Modulation of P-glycoprotein by Stemona alkaloids in human multidrug resistance leukemic cells and structural relationships[J].Phytomedicine,2017,34:182-190
6张囡.药典收载三种百部指纹图谱及止咳作用的比较研究[D].沈阳:沈阳药科大学硕士学位论文,2007
7曹利,卢金清,叶欣,王子禹.HS-SPME-GC-MS联用分析急性子生品和炒制品挥发性成分[J].中国实验方剂学杂志,2017,23(02):69-74
8卢化,张义生,易晨,等.气相色谱-质谱联用技术分析续断及其酒炙品中挥发性成分[J].中国药师,2018,(21)10:1738-1742
9钟瑞敏,肖仔君,张振明,等.小茴香籽精油成分及其抗菌活性研究[J].林产化学与工业,2007。(6):36-40
10南京中医药大学.中药大辞典[M].2版.上海:上海科学技术出版社,2006:347-349
11刘宝双,张纪源.中小型鸡场鸡羽虱的防治[J].中国家禽,1990,(1):16
12张勤,王宗仪.皮下蝇蛆病及其防治[J].河北畜牧兽医,1998,14(1):36-37
13梁忠云,王国聪.草蒿脑资源及生物活性应用研究进展[J].广西林业科学,2010,39(1):49-51
14李红,刘芳.通过SO2和反-2-壬烯醛间的作用来提高啤酒风味稳定性[J].啤酒科技,2002,(9):10-11
15石向慧,纪禄风,伊琳,等.当归挥发油对自发性高血压大鼠肝脏脂代谢的影响[J].解放军药学学报,2017,33(2):113-119
16张金花,熊永爱.丹皮酚对大鼠缺血性心律失常及其心肌细胞miRNA-1表达的影响[J].中国实验方剂学杂志,2015,21(5):129-132
17周建中,王长峰,马勇,等.骨关节炎模型兔膝关节腔注射丹皮酚联合针刺足三里血清白细胞介素1β、肿瘤坏死因子α的变化[J].中国组织工程研究,2012,16(22):4096-4099
18董晓敏,刘布鸣,林霄,等.广西产香茅草挥发油的化学成分分析[J].广西科学,2009,16(3):302-304