薄层色谱与表面增强拉曼光谱联用快速检测辣椒油高脂肪基质中的苏丹红
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摘要
建立了薄层色谱(TLC)与表面增强拉曼光谱(SERS)联用快速检测辣椒油中苏丹红Ⅰ～Ⅳ的方法。用薄层色谱法将苏丹红标准溶液、辣椒油经固相萃取(SPE)后的待测溶液分别进行薄层色谱分离,滴加金纳米棒(GNRs)增强基底后采用拉曼色谱仪检测,利用WiRE 3.4进行光谱收集和基本处理,Origin 8.5.1软件进行数据分析。在最佳的薄层色谱板、拉曼基底及基底的滴加次数条件下,苏丹红的检出限在1 mg/kg以下,实现了高脂肪基质中苏丹红Ⅰ～Ⅳ的快速、灵敏检测,为现场快速检测苏丹红提供了可靠方法。
A rapid detection method using thin layer chromatography(TLC) combined with surface enhanced Raman spectroscopy(SERS) was established for the detection of Sudan dyes illegally added in chili oil.Using TLC to separate Sudan dyes standard solution and extraction of chili oil with solid phase extraction(SPE),the separated spots were detected by Raman spectrometer with gold nanorods substrate.WiRE 3.4 was used for spectral collection,spectra basic processing,and the data analysis was completed with Origin 8.5.1 software.After optimizing several experimental factors of the TLC plate material,different Raman substrates and adding times of substrates,limits of detection of Sudan dyes was obtained to be under 1 mg/kg.The results showed that this method was rapid and sensitive for the detection of four Sudan dyes in high-fat matrix,and provided a reliable method to on-site detection.
A rapid detection method using thin layer chromatography(TLC) combined with surface enhanced Raman spectroscopy(SERS) was established for the detection of Sudan dyes illegally added in chili oil.Using TLC to separate Sudan dyes standard solution and extraction of chili oil with solid phase extraction(SPE),the separated spots were detected by Raman spectrometer with gold nanorods substrate.WiRE 3.4 was used for spectral collection,spectra basic processing,and the data analysis was completed with Origin 8.5.1 software.After optimizing several experimental factors of the TLC plate material,different Raman substrates and adding times of substrates,limits of detection of Sudan dyes was obtained to be under 1 mg/kg.The results showed that this method was rapid and sensitive for the detection of four Sudan dyes in high-fat matrix,and provided a reliable method to on-site detection.
引文
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[2]BOEKHOVEN J,ZHA R H,TANTAKITTI F,et al.Alginate-peptide amphiphile core-shell microparticles as a targeted drug delivery system[J].RSCAdvances,2015,5(12):8753-8756.
[3]肖义夫,顾万江.高效液相色谱法同时测定食品中的金橙Ⅱ和苏丹红Ⅰ~Ⅳ[J].现代预防医学,2007,34(8):1547-1549.
[4]朱成晶,朱鸭梅,李稳,等.LC-MS/MS法检测黑色粮谷类食品中的苏丹黑B和苏丹红B[J].化学分析计量,2015,24(4):58-61.
[5]黄晓兰,吴惠勤,黄芳,等.GC-MS/SIM法同时测定食品中的苏丹红Ⅰ~Ⅳ[J].分析测试学报,2005,24(4):1-5.
[6]CAAMARES M V,REAGAN D A,LOMBARDI JR,et al.TLC-SERS of mauve,the first synthetic dye[J].Journal of Raman Spectroscopy,2014,45(11/12):1147-1152.
[7]朱青霞,曹永兵,曹颖瑛,等.TLC-SERS法快速检测降压类中药中非法添加的四种化学成分[J].光谱学与光谱分析,2014,34(4):990-993.
[8]马枫茹,刘琨,张毅,等.以新型银胶为衬底的超低浓度R6G的拉曼光谱检测[J].光散射学报,2007,19(1):11-15.
[9]JI X,SONG X,LI J,et al.Size control of gold nanocrystals in citrate reduction:the third role of citrate[J].Journal of the American Chemical Society,2007,129(45):13939-13948.
[10]CHEN H,SHAO L,LI Q,et al.Gold nanorods and their plasmonic properties[J].Chemical Society Reviews,2013,42(7):2679.
[11]徐少俊,陈强,陈志龙.金纳米棒合成及其影响因素的研究[J].现代化工,2011,31(S1):160-163.
[12]KEUL H A,M魻LLER M,BOCKSTALLER M RStructural evolution of gold nanorods during controlled secondary growth[J].Langmuir,2007,23(20):10307-10315.
[13]王群,潘文慧,严波,等.薄层色谱-可见分光光度法测定腮红及眼影中苏丹红[J].理化检验(化学分册),2014,50(5):565-568.
[14]LOMBARDI J R,BIRKE R L,LU T,et al Charge-transfer theory of surface enhanced Raman spectroscopy:Herzberg-Teller contributions[J].The Journal of Chemical Physics,1986,84(8):4174-4180.
[15]ADRIAN F J.Charge transfer effects in surface-enhanced Raman scatteringa[J].The Journal of Chemical Physics,1982,77(11):5302-5314.
[16]MOSKOVITS M.Surface-enhanced spectroscopy[J].Reviews of modern physics,1985,57(3):783.
[17]SCHATZ G C.Theoretical studies of surface enhanced Raman scattering[J].Accounts of Chemical Research,1984,17(10):370-376.
[18]MCCALL S L,PLATZMAN P M.Raman scattering from chemisorbed molecules at surfaces[J].Physical Review B,1980,22(4):1660.
[19]LANGGUTH L,SCHOKKER A H,GUO K,et al Plasmonic phase-gradient metasurface for spontaneous emission control[J].Physical Review B,2015,92(20):205401.
[20]BANHOLZER M J,MILLSTONE J E,QIN L,et al.Rationally designed nanos tructures for surfaceenhanced Raman spectroscopy[J].Chemical Society Reviews,2008,37(5):885-897.
[21]GIANNINI V,FERN魣NDEZ-DOM魱NGUEZ A I,HECK S C,et al.Plasmonic nanoantennas:fundamentals and their use in controlling the radiative properties of nanoemitters[J].Chemical Reviews,2011,111(6):3888-3912.
[22]PREZ-JUSTE J,PASTORIZA-SANTOS I,LIZ-MARZ魣N L M,et al.Gold nanorods:synthesis,characterization and applications[J].Coordination Chemistry Reviews,2005,249(17):1870-1901.
[23]LI D,QU L,ZHAI W,et al.Facile on-site detection of substituted aromatic pollutants in water using thin layer chromatography combined with surface-enhanced Raman spectroscopy[J].Environmental Science&Technology,2011,45(9):4046-4052.
[24]GAO F,HU Y,CHEN D,et al.Determination of Sudan I in paprika powder by molecularly imprinted polymers-thin layer chromatography-surface enhanced Raman spectroscopic biosensor[J].Talanta,2015,143:344-352.