摘要
建立了薄层色谱(TLC)与表面增强拉曼光谱(SERS)联用快速检测辣椒油中苏丹红Ⅰ~Ⅳ的方法。用薄层色谱法将苏丹红标准溶液、辣椒油经固相萃取(SPE)后的待测溶液分别进行薄层色谱分离,滴加金纳米棒(GNRs)增强基底后采用拉曼色谱仪检测,利用WiRE 3.4进行光谱收集和基本处理,Origin 8.5.1软件进行数据分析。在最佳的薄层色谱板、拉曼基底及基底的滴加次数条件下,苏丹红的检出限在1 mg/kg以下,实现了高脂肪基质中苏丹红Ⅰ~Ⅳ的快速、灵敏检测,为现场快速检测苏丹红提供了可靠方法。
A rapid detection method using thin layer chromatography(TLC) combined with surface enhanced Raman spectroscopy(SERS) was established for the detection of Sudan dyes illegally added in chili oil.Using TLC to separate Sudan dyes standard solution and extraction of chili oil with solid phase extraction(SPE),the separated spots were detected by Raman spectrometer with gold nanorods substrate.WiRE 3.4 was used for spectral collection,spectra basic processing,and the data analysis was completed with Origin 8.5.1 software.After optimizing several experimental factors of the TLC plate material,different Raman substrates and adding times of substrates,limits of detection of Sudan dyes was obtained to be under 1 mg/kg.The results showed that this method was rapid and sensitive for the detection of four Sudan dyes in high-fat matrix,and provided a reliable method to on-site detection.
引文
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