谷子白发病菌卵孢子重寄生真菌的分离与鉴定
|
摘要
为了从谷子白发病菌卵孢子上分离筛选出对谷子白发病具有生防潜力的重寄生真菌,通过石英砂振荡去除卵孢子表面附着物,利用孢悬液稀释点样和尖端菌丝分离法对重寄生真菌进行分离纯化,采用形态学与真菌r DNA-ITS序列分析对分离出的重寄生真菌进行鉴定。结果表明,谷子白发病菌卵孢子上重寄生r有层出镰孢菌(Fusarium proliferatum)、拟轮枝镰孢菌(Fusarium verticillioides)和半裸镰孢菌(Fusarium incarnatum)3种镰孢属真菌。
The purpose of this research was to isolate the mycoparasite on the oospores from Scolerospora graminicola which with biocontrol potential for foxtail millet downy mildew. The attachment of oospore surface was removed by quartz sand oscillation. The mycoparasite were isolated and purified by spore suspension dilution dot sampling and apical mycelium isolation. The mycoparasite were identified by morphological identification and molecular identification of fungal r DNA-ITS sequence analysis. The results showed that the oospores of foxtail millet downy mildew have Fusarium proliferatum, Fusarium verticillioides and Fusarium incarnatum three strains mycoparasite.
The purpose of this research was to isolate the mycoparasite on the oospores from Scolerospora graminicola which with biocontrol potential for foxtail millet downy mildew. The attachment of oospore surface was removed by quartz sand oscillation. The mycoparasite were isolated and purified by spore suspension dilution dot sampling and apical mycelium isolation. The mycoparasite were identified by morphological identification and molecular identification of fungal r DNA-ITS sequence analysis. The results showed that the oospores of foxtail millet downy mildew have Fusarium proliferatum, Fusarium verticillioides and Fusarium incarnatum three strains mycoparasite.
引文
[1]俞大绂.粟病害[M].北京:科学出版社,1978.
[2]李顺国,刘斐,刘猛,等.近期中国谷子高粱产业发展形势与未来趋势[J].农业展望,2018,14(10):37-40.
[3]宗兆峰,康振生.植物病理学原理[M].北京:中国农业出版社,2010.
[4]卜慕华,夏宏世.关于拔除白尖防治白发病的几个问题[J].中国农业科学,1951(11):21.
[5]李萍花.榆林黄土丘陵区谷子白发病无公害防治技术[J].农业开发与装备, 2016(6):118.
[6]李济宸,杨大俐,甘跃进,等.瑞毒霉(Ridomil)防治谷子白发病(Sclerospora graminicola(Sacc)Schroet)的研究[J].河北农学报,1982,7(4):77-78.
[7]黄云.植物病原真菌的重寄生菌[M].北京:中国农业出版社,2015.
[8]周思彤.生防菌及相关生物技术在植物病害防治中的应用[J].科技经济导刊,2017(4):151.
[9]RAO N N R,PAVGI M S. A mycoparasite on Sclerospora graminicola[J]. Can J Bot,1976,54:220-223
[10]WHIPPS J M. Biology of Coniothyrium minitans and its potential for use in disease biocontrol[J]. Mycol Res,1992,96:897-907.
[11]董汉松.植病研究法[M].北京:中国农业出版社,2012.
[12]李新凤,王建明.镰孢菌的分类鉴定[M].北京:中国农业科学技术出版社,2012.
[13]魏景超.真菌鉴定手册[M].上海:上海科学技术出版社,1979.
[14]BOOTH C. The genus Fusarium[M]. London:Commonwealth Mycological Institute,1971.
[15]曹兴,刘南南,胡燕佩,等.百合鳞茎腐烂病病原菌的鉴定及生物学特性研究[J].河南农业科学,2018,47(12):96-101.
[16]魏蜜,路露,李春琪. 1株油茶病害拮抗真菌的鉴定、生物学特性及拮抗作用研究[J].河南农业科学,2016,45(8):74-80.
[17]周童,袁泽斌,万可,等.园林废弃物中高效降解菌的分离与鉴定[J].天津农业科学,2018,24(10):5-9.
[18]吴晓宗. ITS序列分析法鉴定烟草病株分离出的两种真菌[J].天津农业科学,2016,22(9):133-135.
[19]刘虹,李素玲,董淑英,等.吕梁山乳菇属常见物种的分子鉴定及资源评价[J].山西农业科学,2018,46(10):113-118.
[20]李依韦,银玲. r DNA-ITS序列分析在植物病原真菌分类鉴定中的应用[J].内蒙古民族大学学报(自然科学版),2012,27(1):66-67.
[21]苟凡铖,侯怡铃,宋波,等. r DNA-ITS序列分析法和传统分类法对真菌进行分类鉴定[J].西华师范大学学报(自然科学版),2017,38(4):382-386.
[2]李顺国,刘斐,刘猛,等.近期中国谷子高粱产业发展形势与未来趋势[J].农业展望,2018,14(10):37-40.
[3]宗兆峰,康振生.植物病理学原理[M].北京:中国农业出版社,2010.
[4]卜慕华,夏宏世.关于拔除白尖防治白发病的几个问题[J].中国农业科学,1951(11):21.
[5]李萍花.榆林黄土丘陵区谷子白发病无公害防治技术[J].农业开发与装备, 2016(6):118.
[6]李济宸,杨大俐,甘跃进,等.瑞毒霉(Ridomil)防治谷子白发病(Sclerospora graminicola(Sacc)Schroet)的研究[J].河北农学报,1982,7(4):77-78.
[7]黄云.植物病原真菌的重寄生菌[M].北京:中国农业出版社,2015.
[8]周思彤.生防菌及相关生物技术在植物病害防治中的应用[J].科技经济导刊,2017(4):151.
[9]RAO N N R,PAVGI M S. A mycoparasite on Sclerospora graminicola[J]. Can J Bot,1976,54:220-223
[10]WHIPPS J M. Biology of Coniothyrium minitans and its potential for use in disease biocontrol[J]. Mycol Res,1992,96:897-907.
[11]董汉松.植病研究法[M].北京:中国农业出版社,2012.
[12]李新凤,王建明.镰孢菌的分类鉴定[M].北京:中国农业科学技术出版社,2012.
[13]魏景超.真菌鉴定手册[M].上海:上海科学技术出版社,1979.
[14]BOOTH C. The genus Fusarium[M]. London:Commonwealth Mycological Institute,1971.
[15]曹兴,刘南南,胡燕佩,等.百合鳞茎腐烂病病原菌的鉴定及生物学特性研究[J].河南农业科学,2018,47(12):96-101.
[16]魏蜜,路露,李春琪. 1株油茶病害拮抗真菌的鉴定、生物学特性及拮抗作用研究[J].河南农业科学,2016,45(8):74-80.
[17]周童,袁泽斌,万可,等.园林废弃物中高效降解菌的分离与鉴定[J].天津农业科学,2018,24(10):5-9.
[18]吴晓宗. ITS序列分析法鉴定烟草病株分离出的两种真菌[J].天津农业科学,2016,22(9):133-135.
[19]刘虹,李素玲,董淑英,等.吕梁山乳菇属常见物种的分子鉴定及资源评价[J].山西农业科学,2018,46(10):113-118.
[20]李依韦,银玲. r DNA-ITS序列分析在植物病原真菌分类鉴定中的应用[J].内蒙古民族大学学报(自然科学版),2012,27(1):66-67.
[21]苟凡铖,侯怡铃,宋波,等. r DNA-ITS序列分析法和传统分类法对真菌进行分类鉴定[J].西华师范大学学报(自然科学版),2017,38(4):382-386.