组织芯片技术检测BAG3蛋白在结肠癌组织中的表达及其临床意义
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摘要
目的:探讨Bcl相关抗凋亡蛋白3(又称BAG3蛋白)在结肠癌组织中的表达及其临床意义,阐明BAG3蛋白与结肠癌发生发展的关系。方法:选取90例结肠癌患者的结肠癌组织和癌旁组织,采用组织芯片技术和免疫组织化学SP法检测组织中BAG3蛋白的表达,Kaplan-Meier、单因素和多因素生存分析法分析结肠癌患者临床病理参数与预后的关系。结果:BAG3蛋白在结肠癌组织中呈高表达,90例结肠癌组织中BAG3蛋白阳性表达率为37.8%,癌旁组织中BAG3蛋白阳性表达率为0.01%,两者比较差异有统计学意义(P<0.05)。BAG3蛋白表达与患者性别和肿瘤大小有关联(P<0.05),与患者年龄、病理分级、TNM分期和有无淋巴结转移无关联(P>0.05)。Kaplan-Meier分析,BAG3在结肠癌组织中的表达与患者预后无关联,年龄较小的患者生存率较高,TNM分期低的患者生存率较高。结论:BAG3蛋白在结肠癌组织中呈高表达,蛋白表达与肿瘤大小有关联;BAG3蛋白可能作为结肠癌的肿瘤标志物或治疗靶点。
Objective:To explore the expression of Bcl related anti-apoptotic protein 3(BAG3 protein)in colon cancer and its clinical significance,and to clarify the relationship between its expression and the occurrence and development of colon cancer.Methods:A total of 90 cases of colon cancer tissue and paracancerous tissue were selected,the expressions of BAG3 protein in different tissues were detected by tissue microarray and immunohistochemical SP method;Kaplan-Meier method,univariate analysis and multivariate analysis were used to investigate the relationship between the clinicopathological parameters and the prognosis of the patients with colon cancer.Results:The expression of BAG3 protein in the colon cancer tissue was high,and the positive expression rates of BAG3 protein in the colon cancer tissue and paracancerous tissue were 37.8% and 0.01%,respectively,and the difference was significant(P<0.05).The expression of BAG3 protein was associated with gender and tumor size(P<0.05),but there was no correlation between the expression of BAG3 protein in the colon cancer tissue and age,pathologic grade,TNM stage,and lymph node metastasis(P>0.05).The Kaplan-Meier results showed that the expression of BAG3 protein in the colon cancer tissue was not related with the prognosis of the patients and the survival rates of the younger patients and the higher TNM staging patients were high.Conclusion:The expression of BAG3 protein in the colon cancer tissue is high,and its expression is associated with the tumor size;BAG3 protein may act as a tumor marker and therapeutic target for colon cancer.
Objective:To explore the expression of Bcl related anti-apoptotic protein 3(BAG3 protein)in colon cancer and its clinical significance,and to clarify the relationship between its expression and the occurrence and development of colon cancer.Methods:A total of 90 cases of colon cancer tissue and paracancerous tissue were selected,the expressions of BAG3 protein in different tissues were detected by tissue microarray and immunohistochemical SP method;Kaplan-Meier method,univariate analysis and multivariate analysis were used to investigate the relationship between the clinicopathological parameters and the prognosis of the patients with colon cancer.Results:The expression of BAG3 protein in the colon cancer tissue was high,and the positive expression rates of BAG3 protein in the colon cancer tissue and paracancerous tissue were 37.8% and 0.01%,respectively,and the difference was significant(P<0.05).The expression of BAG3 protein was associated with gender and tumor size(P<0.05),but there was no correlation between the expression of BAG3 protein in the colon cancer tissue and age,pathologic grade,TNM stage,and lymph node metastasis(P>0.05).The Kaplan-Meier results showed that the expression of BAG3 protein in the colon cancer tissue was not related with the prognosis of the patients and the survival rates of the younger patients and the higher TNM staging patients were high.Conclusion:The expression of BAG3 protein in the colon cancer tissue is high,and its expression is associated with the tumor size;BAG3 protein may act as a tumor marker and therapeutic target for colon cancer.
引文
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[2]Shi H,Xu H,Li Z,et al.BAG3 regulates cell proliferation,migration,and invasion in human colorectal cancer[J].Tumour Biol,2016,37(4):5591-5597.
[3]Rosati A,Graziano V,De Laurenzi V,et al.BAG3:a multifaceted protein that regulates major cell pathways[J].Cell Death Dis,2011,2:e141.
[4]强欣,霍文艳,杨灿,等.p16INK4a蛋白在蒙古族患者宫颈病变组织中的表达及其意义[J].吉林大学学报:医学版,2017,43(3):353-355.
[5]Zhu L,Tian GG,Yang Q,et al.Thyroid hormone receptorβ1suppresses proliferation and migration by inhibiting PI3K/Akt signaling in human colorectal cancer cells[J].Oncol Rep,2016,36(3):1419-1426.
[6]Li Q,Wei P,Huang B,et al.MAEL expression links epithelial-mesenchymal transition and stem cell properties in colorectal cancer[J].Int J Cancer,2016,139(11):2502-2511.
[7]张宇,卫子然,杨德君,等.Ras相关区域家族10蛋白在胃癌中的表达及其临床意义[J].第二军医大学学报,2016,37(5).
[8]Oliver Metzig M,Fuchs D,Tagscherer KE,et al.Inhibition of caspases primes colon cancer cells for 5-fluorouracil-induced TNF-α-dependent necroptosis driven by RIP1 kinase and NF-κB[J].Oncogene,2016 35(26):3399-3409.
[9]Lee MY,Kim SY,Choi JS,et al.Induction of Bis,a Bcl-2-binding protein,in reactive astrocytes of the rat hippocampus following kainic acid-induced seizure[J].Exp Mol Med,2002,34(2):167-171.
[10]Arndt V,Dick N,Tawo R,et al.Chaperone-assisted selective autophagy is essential for muscle maintenance[J].Curr Biol,2010,20(2):143-148.
[11]Gamerdinger M,Hajieva P,Kaya AM,et al.Protein quality control during aging involves recruitment of the macroautophagy pathway by BAG3[J].EMBO J,2009,28(7):889-901.
[12]Gamerdinger M,Carra S,Behl C.Emerging roles of molecular chaperones and co-chaperones in selective autophagy:focus on BAG proteins[J].J Mol Med,2011,89(12):1175-1182.
[13]Yang D,Zhou J,Wang H,et al.High expression of BAG3predicts a poor prognosis in human medulloblastoma[J].Tumour Biol,2016,37(10):13215-13224.