cDNA Met基因单核苷酸多态性与结直肠癌易感性关系的研究
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摘要
目的探讨CRC的早期诊断方法。方法选取CRC患者、慢性肠炎患者和正常对照者各50例。采用酚-氯仿法抽提其外周血cDNA,通过PCR定量检测。结果慢性肠炎患者和正常对照组血清β-actin 400 bp及100 bp片段表达量均低于CRC患者(q=42.03～46.23;P<0.05);CRC患者其表达量的高低与患者的年龄、性别、TNM分期、肿瘤数目、CEA等无相关性。结论 cDNA的定量检测可作为早期CRC的诊断方法。
Objective To investigate the early diagnosis of CRC. Methods Fifty patients with CRC, chronic enteritis and normal controls were enrolled. Peripheral blood cDNA was extracted by phenol-chloroform method and quantitatively detected by PCR. Results The expression levels of serum β-actin 400 bp and 100 bp in chronic enteritis patients and normal controls were lower than those in CRC patients(q=42.03～46.23;P<0.05). The expression level of CRC patients was related to the age and gender of the patients. There was no correlation between TNM staging, tumor number, and CEA. Conclusion Quantitative detection of cDNA can be used as a diagnostic method for early CRC.
Objective To investigate the early diagnosis of CRC. Methods Fifty patients with CRC, chronic enteritis and normal controls were enrolled. Peripheral blood cDNA was extracted by phenol-chloroform method and quantitatively detected by PCR. Results The expression levels of serum β-actin 400 bp and 100 bp in chronic enteritis patients and normal controls were lower than those in CRC patients(q=42.03～46.23;P<0.05). The expression level of CRC patients was related to the age and gender of the patients. There was no correlation between TNM staging, tumor number, and CEA. Conclusion Quantitative detection of cDNA can be used as a diagnostic method for early CRC.
引文
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[2] Bauden M,Pamart D,Ansari D,et al.Circulating nucleosomes as epigenetic biomarkers in pancreatic cancer[J].Clinical Epigenetics,2015,7:106.
[3] Han L,Song Q,Jia Y,et al.The clinical significance of systemic inflammation score in esophageal squamous cell carcinoma[J].Tumor Biology,2016,37(3):3081-3090.
[4] Hou X,Du Y,Deng Y,et al.Sleeping Beauty transposon system for genetic etiological research and gene therapy of cancers[J].Cancer biology therapy,2015,16(1):8-16.